Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques
BACKGROUND: Tear desiccation on a glass surface followed by transmitted-light microscopy has served as diagnostic test for dry eye. Four distinctive morphological domains (zones I, II, III and transition band) have been recently recognized in tear microdesiccates. Physicochemical dissimilarities amo...
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| Format: | Article |
| Language: | English |
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BMC
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100028&lng=en&tlng=en |
| _version_ | 1811293696308543488 |
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| author | Felipe Traipe-Salas Leonidas Traipe-Castro Daniela Salinas-Toro Daniela López Felipe Valenzuela Christian Cartes Héctor Toledo-Araya Claudio Pérez Remigio López Solís |
| author_facet | Felipe Traipe-Salas Leonidas Traipe-Castro Daniela Salinas-Toro Daniela López Felipe Valenzuela Christian Cartes Héctor Toledo-Araya Claudio Pérez Remigio López Solís |
| author_sort | Felipe Traipe-Salas |
| collection | DOAJ |
| description | BACKGROUND: Tear desiccation on a glass surface followed by transmitted-light microscopy has served as diagnostic test for dry eye. Four distinctive morphological domains (zones I, II, III and transition band) have been recently recognized in tear microdesiccates. Physicochemical dissimilarities among those domains hamper comprehensive microscopic examination of tear microdesiccates. Optimal observation conditions of entire tear microdesiccates are now investigated. One-μ! aliquots of tear collected from individual healthy eyes were dried at ambient conditions on microscope slides. Tear microdesiccates were examined by combining low-magnification objective lenses with transmitted-light microscopy (brightfield, phase contrasts Ph1,2,3 and darkfield. RESULTS: Fern-like structures (zones II and III) were visible with all illumination methods excepting brightfield. Zone I was the microdesiccate domain displaying the most noticeable illumination-dependent variations, namely transparent band delimited by an outer rim (Ph1, Ph2), homogeneous compactly built structure (brightfield) or invisible domain (darkfield, Ph3). Intermediate positions of the condenser (BF/Ph1, Ph1/Ph2) showed a structured roughly cylindrical zone I. The transition band also varied from invisibility (brightfield) to a well-defined domain comprising interwoven filamentous elements (phase contrasts, darkfield. CONCLUSIONS: Imaging of entire tear microdesiccates by transmitted-light microscopy depends upon illumination. A more comprehensive description of tear microdesiccates can be achieved by combining illumination methods. |
| first_indexed | 2024-04-13T05:05:16Z |
| format | Article |
| id | doaj.art-4889a7b17e9f44b9bebd25b9b579a81c |
| institution | Directory Open Access Journal |
| issn | 0716-9760 |
| language | English |
| last_indexed | 2024-04-13T05:05:16Z |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-4889a7b17e9f44b9bebd25b9b579a81c2022-12-22T03:01:11ZengBMCBiological Research0716-976049011010.1186/s40659-016-0089-0S0716-97602016000100028Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniquesFelipe Traipe-Salas0Leonidas Traipe-Castro1Daniela Salinas-Toro2Daniela López3Felipe Valenzuela4Christian Cartes5Héctor Toledo-Araya6Claudio Pérez7Remigio López Solís8University of ChileFundación Oftalmológica Los AndesFundación Oftalmológica Los AndesFundación Oftalmológica Los AndesFundación Oftalmológica Los AndesFundación Oftalmológica Los AndesUniversity of ChileFundación Oftalmológica Los AndesUniversity of ChileBACKGROUND: Tear desiccation on a glass surface followed by transmitted-light microscopy has served as diagnostic test for dry eye. Four distinctive morphological domains (zones I, II, III and transition band) have been recently recognized in tear microdesiccates. Physicochemical dissimilarities among those domains hamper comprehensive microscopic examination of tear microdesiccates. Optimal observation conditions of entire tear microdesiccates are now investigated. One-μ! aliquots of tear collected from individual healthy eyes were dried at ambient conditions on microscope slides. Tear microdesiccates were examined by combining low-magnification objective lenses with transmitted-light microscopy (brightfield, phase contrasts Ph1,2,3 and darkfield. RESULTS: Fern-like structures (zones II and III) were visible with all illumination methods excepting brightfield. Zone I was the microdesiccate domain displaying the most noticeable illumination-dependent variations, namely transparent band delimited by an outer rim (Ph1, Ph2), homogeneous compactly built structure (brightfield) or invisible domain (darkfield, Ph3). Intermediate positions of the condenser (BF/Ph1, Ph1/Ph2) showed a structured roughly cylindrical zone I. The transition band also varied from invisibility (brightfield) to a well-defined domain comprising interwoven filamentous elements (phase contrasts, darkfield. CONCLUSIONS: Imaging of entire tear microdesiccates by transmitted-light microscopy depends upon illumination. A more comprehensive description of tear microdesiccates can be achieved by combining illumination methods.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100028&lng=en&tlng=enTearTear ferning testTransmitted-light microscopyTear microdesiccateDry eye |
| spellingShingle | Felipe Traipe-Salas Leonidas Traipe-Castro Daniela Salinas-Toro Daniela López Felipe Valenzuela Christian Cartes Héctor Toledo-Araya Claudio Pérez Remigio López Solís Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques Biological Research Tear Tear ferning test Transmitted-light microscopy Tear microdesiccate Dry eye |
| title | Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques |
| title_full | Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques |
| title_fullStr | Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques |
| title_full_unstemmed | Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques |
| title_short | Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques |
| title_sort | progress in tear microdesiccate analysis by combining various transmitted light microscope techniques |
| topic | Tear Tear ferning test Transmitted-light microscopy Tear microdesiccate Dry eye |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100028&lng=en&tlng=en |
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