Genome Studies in Four Species of <i>Calendula</i> L. (Asteraceae) Using Satellite DNAs as Chromosome Markers
The taxonomically challenging genus <i>Calendula</i> L. (Asteraceae) includes lots of medicinal species characterized by their high morphological and karyological variability. For the first time, a repeatome analysis of a valuable medicinal plant <i>Calendula officinalis</i>...
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MDPI AG
2023-12-01
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| Online Access: | https://www.mdpi.com/2223-7747/12/23/4056 |
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| author | Tatiana E. Samatadze Olga Yu. Yurkevich Firdaus M. Khazieva Irina V. Basalaeva Olga M. Savchenko Svyatoslav A. Zoshchuk Alexander I. Morozov Alexandra V. Amosova Olga V. Muravenko |
| author_facet | Tatiana E. Samatadze Olga Yu. Yurkevich Firdaus M. Khazieva Irina V. Basalaeva Olga M. Savchenko Svyatoslav A. Zoshchuk Alexander I. Morozov Alexandra V. Amosova Olga V. Muravenko |
| author_sort | Tatiana E. Samatadze |
| collection | DOAJ |
| description | The taxonomically challenging genus <i>Calendula</i> L. (Asteraceae) includes lots of medicinal species characterized by their high morphological and karyological variability. For the first time, a repeatome analysis of a valuable medicinal plant <i>Calendula officinalis</i> L. was carried out using high-throughput genome DNA sequencing and RepeatExplorer/TAREAN pipelines. The FISH-based visualization of the 45S rDNA, 5S rDNA, and satellite DNAs of <i>C. officinalis</i> was performed on the chromosomes of <i>C. officinalis</i>, <i>C. stellata</i> Cav., <i>C. tripterocarpa</i> Rupr., and <i>C. arvensis</i> L. Three satellite DNAs were demonstrated to be new molecular chromosome markers to study the karyotype structure. Karyograms of the studied species were constructed, their ploidy status was specified, and their relationships were clarified. Our results showed that the <i>C. officinalis</i> karyotype differed from the karyotypes of the other three species, indicating its separate position in the <i>Calendula</i> phylogeny. However, the presence of common repeats revealed in the genomes of all the studied species could be related to their common origin. Our findings demonstrated that <i>C. stellata</i> contributed its genome to allotetraploid <i>C. tripterocarpa</i>, and <i>C. arvensis</i> is an allohexaploid hybrid between <i>C. stellata</i> and <i>C. tripterocarpa</i>. At the same time, further karyotype studies of various <i>Calendula</i> species are required to clarify the pathways of chromosomal reorganization that occurred during speciation. |
| first_indexed | 2024-03-09T01:43:51Z |
| format | Article |
| id | doaj.art-488acbd6bb954cfe864e21f7f7cb5107 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T01:43:51Z |
| publishDate | 2023-12-01 |
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| series | Plants |
| spelling | doaj.art-488acbd6bb954cfe864e21f7f7cb51072023-12-08T15:24:03ZengMDPI AGPlants2223-77472023-12-011223405610.3390/plants12234056Genome Studies in Four Species of <i>Calendula</i> L. (Asteraceae) Using Satellite DNAs as Chromosome MarkersTatiana E. Samatadze0Olga Yu. Yurkevich1Firdaus M. Khazieva2Irina V. Basalaeva3Olga M. Savchenko4Svyatoslav A. Zoshchuk5Alexander I. Morozov6Alexandra V. Amosova7Olga V. Muravenko8Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov St., 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov St., 119991 Moscow, RussiaAll-Russian Institute of Medicinal and Aromatic Plants, 7 Green St., 117216 Moscow, RussiaAll-Russian Institute of Medicinal and Aromatic Plants, 7 Green St., 117216 Moscow, RussiaAll-Russian Institute of Medicinal and Aromatic Plants, 7 Green St., 117216 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov St., 119991 Moscow, RussiaAll-Russian Institute of Medicinal and Aromatic Plants, 7 Green St., 117216 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov St., 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov St., 119991 Moscow, RussiaThe taxonomically challenging genus <i>Calendula</i> L. (Asteraceae) includes lots of medicinal species characterized by their high morphological and karyological variability. For the first time, a repeatome analysis of a valuable medicinal plant <i>Calendula officinalis</i> L. was carried out using high-throughput genome DNA sequencing and RepeatExplorer/TAREAN pipelines. The FISH-based visualization of the 45S rDNA, 5S rDNA, and satellite DNAs of <i>C. officinalis</i> was performed on the chromosomes of <i>C. officinalis</i>, <i>C. stellata</i> Cav., <i>C. tripterocarpa</i> Rupr., and <i>C. arvensis</i> L. Three satellite DNAs were demonstrated to be new molecular chromosome markers to study the karyotype structure. Karyograms of the studied species were constructed, their ploidy status was specified, and their relationships were clarified. Our results showed that the <i>C. officinalis</i> karyotype differed from the karyotypes of the other three species, indicating its separate position in the <i>Calendula</i> phylogeny. However, the presence of common repeats revealed in the genomes of all the studied species could be related to their common origin. Our findings demonstrated that <i>C. stellata</i> contributed its genome to allotetraploid <i>C. tripterocarpa</i>, and <i>C. arvensis</i> is an allohexaploid hybrid between <i>C. stellata</i> and <i>C. tripterocarpa</i>. At the same time, further karyotype studies of various <i>Calendula</i> species are required to clarify the pathways of chromosomal reorganization that occurred during speciation.https://www.mdpi.com/2223-7747/12/23/4056<i>Calendula</i>next-generation sequencingrepeatomesatDNAschromosomefluorescence in situ hybridization |
| spellingShingle | Tatiana E. Samatadze Olga Yu. Yurkevich Firdaus M. Khazieva Irina V. Basalaeva Olga M. Savchenko Svyatoslav A. Zoshchuk Alexander I. Morozov Alexandra V. Amosova Olga V. Muravenko Genome Studies in Four Species of <i>Calendula</i> L. (Asteraceae) Using Satellite DNAs as Chromosome Markers Plants <i>Calendula</i> next-generation sequencing repeatome satDNAs chromosome fluorescence in situ hybridization |
| title | Genome Studies in Four Species of <i>Calendula</i> L. (Asteraceae) Using Satellite DNAs as Chromosome Markers |
| title_full | Genome Studies in Four Species of <i>Calendula</i> L. (Asteraceae) Using Satellite DNAs as Chromosome Markers |
| title_fullStr | Genome Studies in Four Species of <i>Calendula</i> L. (Asteraceae) Using Satellite DNAs as Chromosome Markers |
| title_full_unstemmed | Genome Studies in Four Species of <i>Calendula</i> L. (Asteraceae) Using Satellite DNAs as Chromosome Markers |
| title_short | Genome Studies in Four Species of <i>Calendula</i> L. (Asteraceae) Using Satellite DNAs as Chromosome Markers |
| title_sort | genome studies in four species of i calendula i l asteraceae using satellite dnas as chromosome markers |
| topic | <i>Calendula</i> next-generation sequencing repeatome satDNAs chromosome fluorescence in situ hybridization |
| url | https://www.mdpi.com/2223-7747/12/23/4056 |
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