VALIDATION OF TOTAL COMBUSTION METHOD FOR CRUDE PROTEINS DETERMINATION IN ANIMAL FEED
Crude proteins content is one of the most important parameters of animal feed quality. For total proteins determination Kjeldahl method is the most known and most used. However, total combustion method, well-known as Dumas, is also recognized as standard method. In this paper the model of Dumas vali...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Scientific Veterinary Institute “Novi Sad”
2009-12-01
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| Series: | Archives of Veterinary Medicine |
| Subjects: | |
| Online Access: | https://niv.ns.ac.rs/e-avm/index.php/e-avm/article/view/250 |
| Summary: | Crude proteins content is one of the most important parameters of animal feed quality. For total proteins determination Kjeldahl method is the most known and most used. However, total combustion method, well-known as Dumas, is also recognized as standard method. In this paper the model of Dumas validation for total combustion method on the instrument Elementar- Rapid N is shown. The validation involves determination of linearity, detection limit, accuracy and precision, as the standard required. In validation procedure standard substances were used and the results were processed with suitable statistics methods in Microsoft Excel. Examined validation parameters show that the tested method can be used for accurate determination of total nitrogen (protein) determination in animal feed samples. |
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| ISSN: | 1820-9955 2683-4138 |