VALIDATION OF TOTAL COMBUSTION METHOD FOR CRUDE PROTEINS DETERMINATION IN ANIMAL FEED

Crude proteins content is one of the most important parameters of animal feed quality. For total proteins determination Kjeldahl method is the most known and most used. However, total combustion method, well-known as Dumas, is also recognized as standard method. In this paper the model of Dumas vali...

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Main Authors: Sandra Jakšić, Željko Mihaljev, Milica Živkov-Baloš, Žoran Mašić
Format: Article
Language:English
Published: Scientific Veterinary Institute “Novi Sad” 2009-12-01
Series:Archives of Veterinary Medicine
Subjects:
Online Access:https://niv.ns.ac.rs/e-avm/index.php/e-avm/article/view/250
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author Sandra Jakšić
Željko Mihaljev
Milica Živkov-Baloš
Žoran Mašić
author_facet Sandra Jakšić
Željko Mihaljev
Milica Živkov-Baloš
Žoran Mašić
author_sort Sandra Jakšić
collection DOAJ
description Crude proteins content is one of the most important parameters of animal feed quality. For total proteins determination Kjeldahl method is the most known and most used. However, total combustion method, well-known as Dumas, is also recognized as standard method. In this paper the model of Dumas validation for total combustion method on the instrument Elementar- Rapid N is shown. The validation involves determination of linearity, detection limit, accuracy and precision, as the standard required. In validation procedure standard substances were used and the results were processed with suitable statistics methods in Microsoft Excel. Examined validation parameters show that the tested method can be used for accurate determination of total nitrogen (protein) determination in animal feed samples.
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spelling doaj.art-488e0475246a4242948fe5604fb579002022-12-22T03:10:24ZengScientific Veterinary Institute “Novi Sad”Archives of Veterinary Medicine1820-99552683-41382009-12-012210.46784/e-avm.v2i2.250VALIDATION OF TOTAL COMBUSTION METHOD FOR CRUDE PROTEINS DETERMINATION IN ANIMAL FEEDSandra Jakšić0Željko Mihaljev1Milica Živkov-Baloš2Žoran Mašić3Scientific Veterinary Institute "Novi Sad", Novi Sad, Rumenački put 20Scientific Veterinary Institute "Novi Sad", Novi Sad, Rumenački put 20Scientific Veterinary Institute "Novi Sad", Novi Sad, Rumenački put 20Faculty of Agriculture, Novi SadCrude proteins content is one of the most important parameters of animal feed quality. For total proteins determination Kjeldahl method is the most known and most used. However, total combustion method, well-known as Dumas, is also recognized as standard method. In this paper the model of Dumas validation for total combustion method on the instrument Elementar- Rapid N is shown. The validation involves determination of linearity, detection limit, accuracy and precision, as the standard required. In validation procedure standard substances were used and the results were processed with suitable statistics methods in Microsoft Excel. Examined validation parameters show that the tested method can be used for accurate determination of total nitrogen (protein) determination in animal feed samples.https://niv.ns.ac.rs/e-avm/index.php/e-avm/article/view/250crude proteinstotal combustion methodvalidationanimal feed
spellingShingle Sandra Jakšić
Željko Mihaljev
Milica Živkov-Baloš
Žoran Mašić
VALIDATION OF TOTAL COMBUSTION METHOD FOR CRUDE PROTEINS DETERMINATION IN ANIMAL FEED
Archives of Veterinary Medicine
crude proteins
total combustion method
validation
animal feed
title VALIDATION OF TOTAL COMBUSTION METHOD FOR CRUDE PROTEINS DETERMINATION IN ANIMAL FEED
title_full VALIDATION OF TOTAL COMBUSTION METHOD FOR CRUDE PROTEINS DETERMINATION IN ANIMAL FEED
title_fullStr VALIDATION OF TOTAL COMBUSTION METHOD FOR CRUDE PROTEINS DETERMINATION IN ANIMAL FEED
title_full_unstemmed VALIDATION OF TOTAL COMBUSTION METHOD FOR CRUDE PROTEINS DETERMINATION IN ANIMAL FEED
title_short VALIDATION OF TOTAL COMBUSTION METHOD FOR CRUDE PROTEINS DETERMINATION IN ANIMAL FEED
title_sort validation of total combustion method for crude proteins determination in animal feed
topic crude proteins
total combustion method
validation
animal feed
url https://niv.ns.ac.rs/e-avm/index.php/e-avm/article/view/250
work_keys_str_mv AT sandrajaksic validationoftotalcombustionmethodforcrudeproteinsdeterminationinanimalfeed
AT zeljkomihaljev validationoftotalcombustionmethodforcrudeproteinsdeterminationinanimalfeed
AT milicazivkovbalos validationoftotalcombustionmethodforcrudeproteinsdeterminationinanimalfeed
AT zoranmasic validationoftotalcombustionmethodforcrudeproteinsdeterminationinanimalfeed