Phytochemical screening and characterization of the antioxidant, anti-proliferative and antibacterial effects of different extracts of Opuntia ficus-indica peel
Objectives: Opuntia ficus-indica peels need further investigations as they could contain multiple active molecules. Bioactive secondary metabolites are important for health maintenance and play a significant role in treating some common diseases. Hence, the present study analyzed the chemical compos...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722003974 |
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| author | Shimaa K. Ali Sara M. Mahmoud Samar S. El-Masry Dalal Hussien M. Alkhalifah Wael N. Hozzein Moustafa A. Aboel-Ainin |
| author_facet | Shimaa K. Ali Sara M. Mahmoud Samar S. El-Masry Dalal Hussien M. Alkhalifah Wael N. Hozzein Moustafa A. Aboel-Ainin |
| author_sort | Shimaa K. Ali |
| collection | DOAJ |
| description | Objectives: Opuntia ficus-indica peels need further investigations as they could contain multiple active molecules. Bioactive secondary metabolites are important for health maintenance and play a significant role in treating some common diseases. Hence, the present study analyzed the chemical composition and biological activities of the plant extract using in vitro models. Methods: The antioxidant assays were estimated in terms of DPPH radical scavenging; the chemical characterization is done in terms of total polyphenol and flavonoids, as well as HPLC characterization. The SRB assay was used for anticancer analysis whereas disc diffusion and minimum inhibitory concentration was determined to assess antimicrobial activity. Results: The content of total polyphenol (TPC) and flavonoids (TFC) differed significantly in Opuntia ficus-indica peel cyclohexanone extract (OFICE), Opuntia ficus-indica peel 70 % ethanol in water extract (OFI70EE), and Opuntia ficus-indica peel 70 % ethanol in water extract (OFI100EE)), with varying values of TPC and TFC. In HPLC analysis, there observed the presence of 8 phenolic and flavonoids compounds. Antioxidant analysis showed that OFICE has higher antioxidant activity. Bioassay based on MCF-7 showed that O. ficus-indica extracts significantly (p < 0.05) reduced cell viability of cancer cells at 400 µg/mL after 72 h of incubation time. Concerning the antibacterial activity, the cyclohexanone extract gave the highest antibacterial inhibition as compared to the other extracts, and the highest activity was recorded against Listeria monocytogenes. Also, the Opuntia ficus-indica peel extracts showed potential antiviral activity with the mean inhibition rates (39, 19, and 67.66 %) and (35, 16.66, and 54.66 %) against H5N1 and rotavirus respectively. Conclusion: Overall, the study confirms the antioxidant, antimicrobial and anticancer properties of the extract and further studies in animal models are necessary to validate their pharmacological applications. |
| first_indexed | 2024-04-11T21:12:50Z |
| format | Article |
| id | doaj.art-488e31dbbb99437bb81bf2347ba74ff3 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-11T21:12:50Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-488e31dbbb99437bb81bf2347ba74ff32022-12-22T04:02:58ZengElsevierJournal of King Saud University: Science1018-36472022-10-01347102216Phytochemical screening and characterization of the antioxidant, anti-proliferative and antibacterial effects of different extracts of Opuntia ficus-indica peelShimaa K. Ali0Sara M. Mahmoud1Samar S. El-Masry2Dalal Hussien M. Alkhalifah3Wael N. Hozzein4Moustafa A. Aboel-Ainin5Department of Agricultural Microbiology, Faculty of Agriculture, Beni-Suef University, Beni-Suef 62521, EgyptDepartment of Biotechnology, Faculty of Graduate Studies and Environmental Researches, Ain Shams University, Cairo 11311, EgyptDep. of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, Cairo 11311, EgyptDepartment of Biology, College of Science, Princess Nourah Bint Abdulrahman University, B.O. Box 84428, Riyadh 11671, Saudi ArabiaBotany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, EgyptDepartment of Biochemistry, Faculty of Agriculture, Beni-Suef University, Beni-Suef 62521, Egypt; Corresponding author.Objectives: Opuntia ficus-indica peels need further investigations as they could contain multiple active molecules. Bioactive secondary metabolites are important for health maintenance and play a significant role in treating some common diseases. Hence, the present study analyzed the chemical composition and biological activities of the plant extract using in vitro models. Methods: The antioxidant assays were estimated in terms of DPPH radical scavenging; the chemical characterization is done in terms of total polyphenol and flavonoids, as well as HPLC characterization. The SRB assay was used for anticancer analysis whereas disc diffusion and minimum inhibitory concentration was determined to assess antimicrobial activity. Results: The content of total polyphenol (TPC) and flavonoids (TFC) differed significantly in Opuntia ficus-indica peel cyclohexanone extract (OFICE), Opuntia ficus-indica peel 70 % ethanol in water extract (OFI70EE), and Opuntia ficus-indica peel 70 % ethanol in water extract (OFI100EE)), with varying values of TPC and TFC. In HPLC analysis, there observed the presence of 8 phenolic and flavonoids compounds. Antioxidant analysis showed that OFICE has higher antioxidant activity. Bioassay based on MCF-7 showed that O. ficus-indica extracts significantly (p < 0.05) reduced cell viability of cancer cells at 400 µg/mL after 72 h of incubation time. Concerning the antibacterial activity, the cyclohexanone extract gave the highest antibacterial inhibition as compared to the other extracts, and the highest activity was recorded against Listeria monocytogenes. Also, the Opuntia ficus-indica peel extracts showed potential antiviral activity with the mean inhibition rates (39, 19, and 67.66 %) and (35, 16.66, and 54.66 %) against H5N1 and rotavirus respectively. Conclusion: Overall, the study confirms the antioxidant, antimicrobial and anticancer properties of the extract and further studies in animal models are necessary to validate their pharmacological applications.http://www.sciencedirect.com/science/article/pii/S1018364722003974Opuntia ficus-indicaTotal phenolsTotal flavonoidsMTTAntioxidantAntitumor |
| spellingShingle | Shimaa K. Ali Sara M. Mahmoud Samar S. El-Masry Dalal Hussien M. Alkhalifah Wael N. Hozzein Moustafa A. Aboel-Ainin Phytochemical screening and characterization of the antioxidant, anti-proliferative and antibacterial effects of different extracts of Opuntia ficus-indica peel Journal of King Saud University: Science Opuntia ficus-indica Total phenols Total flavonoids MTT Antioxidant Antitumor |
| title | Phytochemical screening and characterization of the antioxidant, anti-proliferative and antibacterial effects of different extracts of Opuntia ficus-indica peel |
| title_full | Phytochemical screening and characterization of the antioxidant, anti-proliferative and antibacterial effects of different extracts of Opuntia ficus-indica peel |
| title_fullStr | Phytochemical screening and characterization of the antioxidant, anti-proliferative and antibacterial effects of different extracts of Opuntia ficus-indica peel |
| title_full_unstemmed | Phytochemical screening and characterization of the antioxidant, anti-proliferative and antibacterial effects of different extracts of Opuntia ficus-indica peel |
| title_short | Phytochemical screening and characterization of the antioxidant, anti-proliferative and antibacterial effects of different extracts of Opuntia ficus-indica peel |
| title_sort | phytochemical screening and characterization of the antioxidant anti proliferative and antibacterial effects of different extracts of opuntia ficus indica peel |
| topic | Opuntia ficus-indica Total phenols Total flavonoids MTT Antioxidant Antitumor |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722003974 |
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