Novel Copper Oxide Bio-Nanocrystals to Target Outer Membrane Lectin of Vancomycin-Resistant <i>Enterococcus faecium</i> (VREfm): In Silico, Bioavailability, Antimicrobial, and Anticancer Potential
In present study, we used <i>Olea europaea</i> leaf extract to biosynthesize in situ Copper Oxide nanocrystals (<i>CuO @OVLe NCs</i>) with powerful antibacterial and anti-cancer capabilities. Physio-chemical analyses, such as UV/Vis, FTIR, XRD, EDX, SEM, and TEM, were applied...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/22/7957 |
| _version_ | 1797464413929734144 |
|---|---|
| author | Mahmoud Kandeel Mohamed Sharaf Arshad Mahdi Hamad Ahmad O. Babalghith Mohnad Abdalla Muhammad Arif Reem Binsuwaidan Nashwah G. M. Attallah Hossam Aladl Aladl Aladl Samy Selim Mariusz Jaremko |
| author_facet | Mahmoud Kandeel Mohamed Sharaf Arshad Mahdi Hamad Ahmad O. Babalghith Mohnad Abdalla Muhammad Arif Reem Binsuwaidan Nashwah G. M. Attallah Hossam Aladl Aladl Aladl Samy Selim Mariusz Jaremko |
| author_sort | Mahmoud Kandeel |
| collection | DOAJ |
| description | In present study, we used <i>Olea europaea</i> leaf extract to biosynthesize in situ Copper Oxide nanocrystals (<i>CuO @OVLe NCs</i>) with powerful antibacterial and anti-cancer capabilities. Physio-chemical analyses, such as UV/Vis, FTIR, XRD, EDX, SEM, and TEM, were applied to characterize <i>CuO @OVLe NCs</i>. The UV/Vis spectrum demonstrated a strong peak at 345 nm. Furthermore, FTIR, XRD, and EDX validated the coating operation’s contact with colloidal <i>CuO @OVLe NCs</i>. According to TEM and SEM analyses, <i>CuO @OVLe NCs</i> exhibited a spherical shape and uniform distribution of size with aggregation, for an average size of ~75 nm. The nanoparticles demonstrated a considerable antibacterial effect against <i>E. faecium</i> bacterial growth, as well as an increased inhibition rate in a dose-dependent manner on the MCF-7, PC3, and HpeG2 cancer cell lines and a decreased inhibition rate on WRL-68. Molecular docking and MD simulation were used to demonstrate the high binding affinity of a ligand (Oleuropein) toward the lectin receptor complex of the outer membrane to vancomycin-resistant <i>E. faecium</i> (VREfm) via amino acids (Leu 195, Thr 288, His 165, and Ser 196). Hence, our results expand the accessibility of <i>OVLe’s</i> bioactive components as a promising natural source for the manufacture of physiologically active components and the creation of green biosynthesis of metal nanocrystals. |
| first_indexed | 2024-03-09T18:06:48Z |
| format | Article |
| id | doaj.art-488ff68f204c40dc95ce25a1c25aef12 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:06:48Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-488ff68f204c40dc95ce25a1c25aef122023-11-24T09:24:01ZengMDPI AGMolecules1420-30492022-11-012722795710.3390/molecules27227957Novel Copper Oxide Bio-Nanocrystals to Target Outer Membrane Lectin of Vancomycin-Resistant <i>Enterococcus faecium</i> (VREfm): In Silico, Bioavailability, Antimicrobial, and Anticancer PotentialMahmoud Kandeel0Mohamed Sharaf1Arshad Mahdi Hamad2Ahmad O. Babalghith3Mohnad Abdalla4Muhammad Arif5Reem Binsuwaidan6Nashwah G. M. Attallah7Hossam Aladl Aladl Aladl8Samy Selim9Mariusz Jaremko10Department of Biomedical Sciences, College of Veterinary Medicine, King Faisal University, Al-Hofuf 31982, Saudi ArabiaDepartment of Biochemistry, Faculty of Agriculture, AL-Azhar University, Nasr City, Cairo 11651, EgyptDepartment of Biology, College of Science, Tikrit University, Tikrit 34001, IraqMedical Genetics Department, College of Medicine, Umm Al-Qura University, Mecca 24382, Saudi ArabiaPediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan 250022, ChinaDepartment of Biochemistry and Molecular Biology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaDepartment of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi ArabiaEgyptian Drug Authority (EDA) Previously NODCAR, Giza 12513, EgyptDepartment of Internal Medicine, Faculty of Medicine, AL-Azhar University, Nasr City, Cairo 11651, EgyptDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72388, Saudi ArabiaSmart-Health Initiative and Red Sea Research Center, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi ArabiaIn present study, we used <i>Olea europaea</i> leaf extract to biosynthesize in situ Copper Oxide nanocrystals (<i>CuO @OVLe NCs</i>) with powerful antibacterial and anti-cancer capabilities. Physio-chemical analyses, such as UV/Vis, FTIR, XRD, EDX, SEM, and TEM, were applied to characterize <i>CuO @OVLe NCs</i>. The UV/Vis spectrum demonstrated a strong peak at 345 nm. Furthermore, FTIR, XRD, and EDX validated the coating operation’s contact with colloidal <i>CuO @OVLe NCs</i>. According to TEM and SEM analyses, <i>CuO @OVLe NCs</i> exhibited a spherical shape and uniform distribution of size with aggregation, for an average size of ~75 nm. The nanoparticles demonstrated a considerable antibacterial effect against <i>E. faecium</i> bacterial growth, as well as an increased inhibition rate in a dose-dependent manner on the MCF-7, PC3, and HpeG2 cancer cell lines and a decreased inhibition rate on WRL-68. Molecular docking and MD simulation were used to demonstrate the high binding affinity of a ligand (Oleuropein) toward the lectin receptor complex of the outer membrane to vancomycin-resistant <i>E. faecium</i> (VREfm) via amino acids (Leu 195, Thr 288, His 165, and Ser 196). Hence, our results expand the accessibility of <i>OVLe’s</i> bioactive components as a promising natural source for the manufacture of physiologically active components and the creation of green biosynthesis of metal nanocrystals.https://www.mdpi.com/1420-3049/27/22/7957nanocrystal<i>Olea europaea</i><i>Enterococcus faecium</i>green biosynthesisantimicrobialanti-cancer |
| spellingShingle | Mahmoud Kandeel Mohamed Sharaf Arshad Mahdi Hamad Ahmad O. Babalghith Mohnad Abdalla Muhammad Arif Reem Binsuwaidan Nashwah G. M. Attallah Hossam Aladl Aladl Aladl Samy Selim Mariusz Jaremko Novel Copper Oxide Bio-Nanocrystals to Target Outer Membrane Lectin of Vancomycin-Resistant <i>Enterococcus faecium</i> (VREfm): In Silico, Bioavailability, Antimicrobial, and Anticancer Potential Molecules nanocrystal <i>Olea europaea</i> <i>Enterococcus faecium</i> green biosynthesis antimicrobial anti-cancer |
| title | Novel Copper Oxide Bio-Nanocrystals to Target Outer Membrane Lectin of Vancomycin-Resistant <i>Enterococcus faecium</i> (VREfm): In Silico, Bioavailability, Antimicrobial, and Anticancer Potential |
| title_full | Novel Copper Oxide Bio-Nanocrystals to Target Outer Membrane Lectin of Vancomycin-Resistant <i>Enterococcus faecium</i> (VREfm): In Silico, Bioavailability, Antimicrobial, and Anticancer Potential |
| title_fullStr | Novel Copper Oxide Bio-Nanocrystals to Target Outer Membrane Lectin of Vancomycin-Resistant <i>Enterococcus faecium</i> (VREfm): In Silico, Bioavailability, Antimicrobial, and Anticancer Potential |
| title_full_unstemmed | Novel Copper Oxide Bio-Nanocrystals to Target Outer Membrane Lectin of Vancomycin-Resistant <i>Enterococcus faecium</i> (VREfm): In Silico, Bioavailability, Antimicrobial, and Anticancer Potential |
| title_short | Novel Copper Oxide Bio-Nanocrystals to Target Outer Membrane Lectin of Vancomycin-Resistant <i>Enterococcus faecium</i> (VREfm): In Silico, Bioavailability, Antimicrobial, and Anticancer Potential |
| title_sort | novel copper oxide bio nanocrystals to target outer membrane lectin of vancomycin resistant i enterococcus faecium i vrefm in silico bioavailability antimicrobial and anticancer potential |
| topic | nanocrystal <i>Olea europaea</i> <i>Enterococcus faecium</i> green biosynthesis antimicrobial anti-cancer |
| url | https://www.mdpi.com/1420-3049/27/22/7957 |
| work_keys_str_mv | AT mahmoudkandeel novelcopperoxidebionanocrystalstotargetoutermembranelectinofvancomycinresistantienterococcusfaeciumivrefminsilicobioavailabilityantimicrobialandanticancerpotential AT mohamedsharaf novelcopperoxidebionanocrystalstotargetoutermembranelectinofvancomycinresistantienterococcusfaeciumivrefminsilicobioavailabilityantimicrobialandanticancerpotential AT arshadmahdihamad novelcopperoxidebionanocrystalstotargetoutermembranelectinofvancomycinresistantienterococcusfaeciumivrefminsilicobioavailabilityantimicrobialandanticancerpotential AT ahmadobabalghith novelcopperoxidebionanocrystalstotargetoutermembranelectinofvancomycinresistantienterococcusfaeciumivrefminsilicobioavailabilityantimicrobialandanticancerpotential AT mohnadabdalla novelcopperoxidebionanocrystalstotargetoutermembranelectinofvancomycinresistantienterococcusfaeciumivrefminsilicobioavailabilityantimicrobialandanticancerpotential AT muhammadarif novelcopperoxidebionanocrystalstotargetoutermembranelectinofvancomycinresistantienterococcusfaeciumivrefminsilicobioavailabilityantimicrobialandanticancerpotential AT reembinsuwaidan novelcopperoxidebionanocrystalstotargetoutermembranelectinofvancomycinresistantienterococcusfaeciumivrefminsilicobioavailabilityantimicrobialandanticancerpotential AT nashwahgmattallah novelcopperoxidebionanocrystalstotargetoutermembranelectinofvancomycinresistantienterococcusfaeciumivrefminsilicobioavailabilityantimicrobialandanticancerpotential AT hossamaladlaladlaladl novelcopperoxidebionanocrystalstotargetoutermembranelectinofvancomycinresistantienterococcusfaeciumivrefminsilicobioavailabilityantimicrobialandanticancerpotential AT samyselim novelcopperoxidebionanocrystalstotargetoutermembranelectinofvancomycinresistantienterococcusfaeciumivrefminsilicobioavailabilityantimicrobialandanticancerpotential AT mariuszjaremko novelcopperoxidebionanocrystalstotargetoutermembranelectinofvancomycinresistantienterococcusfaeciumivrefminsilicobioavailabilityantimicrobialandanticancerpotential |