Surface functionalization-dependent localization and affinity of SiO2 nanoparticles within the biofilm EPS matrix
The contribution of the biofilm extracellular polymeric substance (EPS) matrix to reduced antimicrobial susceptibility in biofilms is widely recognised. As such, the direct targeting of the EPS matrix is a promising biofilm control strategy that allows for the disruption of the matrix, thereby allow...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Biofilm |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207520300113 |
| _version_ | 1818917348916068352 |
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| author | Dishon Wayne Hiebner Caio Barros Laura Quinn Stefania Vitale Eoin Casey |
| author_facet | Dishon Wayne Hiebner Caio Barros Laura Quinn Stefania Vitale Eoin Casey |
| author_sort | Dishon Wayne Hiebner |
| collection | DOAJ |
| description | The contribution of the biofilm extracellular polymeric substance (EPS) matrix to reduced antimicrobial susceptibility in biofilms is widely recognised. As such, the direct targeting of the EPS matrix is a promising biofilm control strategy that allows for the disruption of the matrix, thereby allowing a subsequent increase in susceptibility to antimicrobial agents. To this end, surface-functionalized nanoparticles (NPs) have received considerable attention. However, the fundamental understanding of the interactions occurring between engineered NPs and the biofilm EPS matrix has not yet been fully elucidated. An insight into the underlying mechanisms involved when a NP interacts with the EPS matrix will aid in the design of more efficient NPs for biofilm control. Here we demonstrate the use of highly specific fluorescent probes in confocal laser scanning microscopy (CLSM) to illustrate the distribution of EPS macromolecules within the biofilm. Thereafter, a three-dimensional (3D) colocalization analysis was used to assess the affinity of differently functionalized silica NPs (SiNPs) and EPS macromolecules from Pseudomonas fluorescens biofilms. Results show that both the charge and surface functional groups of SiNPs dramatically affected the extent to which SiNPs interacted and localized with EPS macromolecules, including proteins, polysaccharides and DNA. Hypotheses are also presented about the possible physicochemical interactions which may be dominant in EPS matrix-NP interactions. This research not only develops an innovative CLSM-based methodology for elucidating biofilm-nanoparticle interactions but also provides a platform on which to build more efficient NP systems for biofilm control. |
| first_indexed | 2024-12-20T00:32:39Z |
| format | Article |
| id | doaj.art-4898b489e80f44199245bae40868f429 |
| institution | Directory Open Access Journal |
| issn | 2590-2075 |
| language | English |
| last_indexed | 2024-12-20T00:32:39Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj.art-4898b489e80f44199245bae40868f4292022-12-21T19:59:53ZengElsevierBiofilm2590-20752020-12-012100029Surface functionalization-dependent localization and affinity of SiO2 nanoparticles within the biofilm EPS matrixDishon Wayne Hiebner0Caio Barros1Laura Quinn2Stefania Vitale3Eoin Casey4UCD School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin 4, Dublin, IrelandUCD School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin 4, Dublin, IrelandUCD School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin 4, Dublin, IrelandCorresponding author.; UCD School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin 4, Dublin, IrelandUCD School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin 4, Dublin, IrelandThe contribution of the biofilm extracellular polymeric substance (EPS) matrix to reduced antimicrobial susceptibility in biofilms is widely recognised. As such, the direct targeting of the EPS matrix is a promising biofilm control strategy that allows for the disruption of the matrix, thereby allowing a subsequent increase in susceptibility to antimicrobial agents. To this end, surface-functionalized nanoparticles (NPs) have received considerable attention. However, the fundamental understanding of the interactions occurring between engineered NPs and the biofilm EPS matrix has not yet been fully elucidated. An insight into the underlying mechanisms involved when a NP interacts with the EPS matrix will aid in the design of more efficient NPs for biofilm control. Here we demonstrate the use of highly specific fluorescent probes in confocal laser scanning microscopy (CLSM) to illustrate the distribution of EPS macromolecules within the biofilm. Thereafter, a three-dimensional (3D) colocalization analysis was used to assess the affinity of differently functionalized silica NPs (SiNPs) and EPS macromolecules from Pseudomonas fluorescens biofilms. Results show that both the charge and surface functional groups of SiNPs dramatically affected the extent to which SiNPs interacted and localized with EPS macromolecules, including proteins, polysaccharides and DNA. Hypotheses are also presented about the possible physicochemical interactions which may be dominant in EPS matrix-NP interactions. This research not only develops an innovative CLSM-based methodology for elucidating biofilm-nanoparticle interactions but also provides a platform on which to build more efficient NP systems for biofilm control.http://www.sciencedirect.com/science/article/pii/S2590207520300113Bacterial biofilmNanoparticlesPseudomonas fluorescensEPS matrixPhysicochemical interactionsColocalization |
| spellingShingle | Dishon Wayne Hiebner Caio Barros Laura Quinn Stefania Vitale Eoin Casey Surface functionalization-dependent localization and affinity of SiO2 nanoparticles within the biofilm EPS matrix Biofilm Bacterial biofilm Nanoparticles Pseudomonas fluorescens EPS matrix Physicochemical interactions Colocalization |
| title | Surface functionalization-dependent localization and affinity of SiO2 nanoparticles within the biofilm EPS matrix |
| title_full | Surface functionalization-dependent localization and affinity of SiO2 nanoparticles within the biofilm EPS matrix |
| title_fullStr | Surface functionalization-dependent localization and affinity of SiO2 nanoparticles within the biofilm EPS matrix |
| title_full_unstemmed | Surface functionalization-dependent localization and affinity of SiO2 nanoparticles within the biofilm EPS matrix |
| title_short | Surface functionalization-dependent localization and affinity of SiO2 nanoparticles within the biofilm EPS matrix |
| title_sort | surface functionalization dependent localization and affinity of sio2 nanoparticles within the biofilm eps matrix |
| topic | Bacterial biofilm Nanoparticles Pseudomonas fluorescens EPS matrix Physicochemical interactions Colocalization |
| url | http://www.sciencedirect.com/science/article/pii/S2590207520300113 |
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