Duplication of a Conditionally Dispensable Chromosome Carrying Pea Pathogenicity (PEP) Gene Clusters in Nectria haematococca
A supernumerary chromosome called a conditionally dispensable chromosome (CDC) is essential for pathogenicity of Nectria haematococca on pea. Among several CDCs discovered in N. haematococca, the PDA1 CDC that harbors the pisatin demethylation gene PDA1 is one of the best-studied CDCs and serves as...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2007-12-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-12-1495 |
| _version_ | 1818915221203320832 |
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| author | Hamid S. Garmaroodi Masatoki Taga |
| author_facet | Hamid S. Garmaroodi Masatoki Taga |
| author_sort | Hamid S. Garmaroodi |
| collection | DOAJ |
| description | A supernumerary chromosome called a conditionally dispensable chromosome (CDC) is essential for pathogenicity of Nectria haematococca on pea. Among several CDCs discovered in N. haematococca, the PDA1 CDC that harbors the pisatin demethylation gene PDA1 is one of the best-studied CDCs and serves as a model for plant-pathogenic fungi. Although the presence of multiple copies is usual for supernumerary chromosomes in other eukaryotes, this possibility has not been examined well for any CDCs in N. haematococca. In this study, we produced strains with multiple copies of the PDA1 CDC by protoplast fusion and analyzed dosage effects of this chromosome. Using multiple methods, including cytological chromosome counting and fluorescence in situ hybridization, the fusion products between two transformants derived from the same strain that bears a single PDA1 CDC were shown to contain two PDA1 CDCs from both transformants and estimated to be haploid resulting from the deletion of an extra set or sets of A chromosomes in the fused nuclei. In phenotype assays, dosage effects of PDA1 CDC in the fusion products were evident as increased virulence and homoserine-utilizing ability compared with the parents. In a separate fusion experiment, PDA1 CDC accumulated up to four copies in a haploid genome. |
| first_indexed | 2024-12-19T23:58:50Z |
| format | Article |
| id | doaj.art-4899abf38c8f4f4a9054daaac4e7d5dc |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:58:50Z |
| publishDate | 2007-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-4899abf38c8f4f4a9054daaac4e7d5dc2022-12-21T20:00:55ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062007-12-0120121495150410.1094/MPMI-20-12-1495Duplication of a Conditionally Dispensable Chromosome Carrying Pea Pathogenicity (PEP) Gene Clusters in Nectria haematococcaHamid S. GarmaroodiMasatoki TagaA supernumerary chromosome called a conditionally dispensable chromosome (CDC) is essential for pathogenicity of Nectria haematococca on pea. Among several CDCs discovered in N. haematococca, the PDA1 CDC that harbors the pisatin demethylation gene PDA1 is one of the best-studied CDCs and serves as a model for plant-pathogenic fungi. Although the presence of multiple copies is usual for supernumerary chromosomes in other eukaryotes, this possibility has not been examined well for any CDCs in N. haematococca. In this study, we produced strains with multiple copies of the PDA1 CDC by protoplast fusion and analyzed dosage effects of this chromosome. Using multiple methods, including cytological chromosome counting and fluorescence in situ hybridization, the fusion products between two transformants derived from the same strain that bears a single PDA1 CDC were shown to contain two PDA1 CDCs from both transformants and estimated to be haploid resulting from the deletion of an extra set or sets of A chromosomes in the fused nuclei. In phenotype assays, dosage effects of PDA1 CDC in the fusion products were evident as increased virulence and homoserine-utilizing ability compared with the parents. In a separate fusion experiment, PDA1 CDC accumulated up to four copies in a haploid genome.https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-12-1495FISHFusarium solanigerm tube burst method |
| spellingShingle | Hamid S. Garmaroodi Masatoki Taga Duplication of a Conditionally Dispensable Chromosome Carrying Pea Pathogenicity (PEP) Gene Clusters in Nectria haematococca Molecular Plant-Microbe Interactions FISH Fusarium solani germ tube burst method |
| title | Duplication of a Conditionally Dispensable Chromosome Carrying Pea Pathogenicity (PEP) Gene Clusters in Nectria haematococca |
| title_full | Duplication of a Conditionally Dispensable Chromosome Carrying Pea Pathogenicity (PEP) Gene Clusters in Nectria haematococca |
| title_fullStr | Duplication of a Conditionally Dispensable Chromosome Carrying Pea Pathogenicity (PEP) Gene Clusters in Nectria haematococca |
| title_full_unstemmed | Duplication of a Conditionally Dispensable Chromosome Carrying Pea Pathogenicity (PEP) Gene Clusters in Nectria haematococca |
| title_short | Duplication of a Conditionally Dispensable Chromosome Carrying Pea Pathogenicity (PEP) Gene Clusters in Nectria haematococca |
| title_sort | duplication of a conditionally dispensable chromosome carrying pea pathogenicity pep gene clusters in nectria haematococca |
| topic | FISH Fusarium solani germ tube burst method |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-12-1495 |
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