Ginsenoside Rg1 promoted the wound healing in diabetic foot ulcers via miR-489–3p/Sirt1 axis
Purpose: Diabetic foot ulcers (DFUs) are common complications of high severity for diabetes. Ginsenoside Rg1 (Rg1) has the potential for diabetes and cardiovascular diseases therapy. This research aimed at exploring the regulation of Rg1 on DFUs treatment and the underlying mechanism. Methods: Human...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Journal of Pharmacological Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S134786132100075X |
| _version_ | 1818716189552017408 |
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| author | Liang Huang Hua-An Cai Ming-Sheng Zhang Ruo-Yi Liao Xing Huang Feng-Dan Hu |
| author_facet | Liang Huang Hua-An Cai Ming-Sheng Zhang Ruo-Yi Liao Xing Huang Feng-Dan Hu |
| author_sort | Liang Huang |
| collection | DOAJ |
| description | Purpose: Diabetic foot ulcers (DFUs) are common complications of high severity for diabetes. Ginsenoside Rg1 (Rg1) has the potential for diabetes and cardiovascular diseases therapy. This research aimed at exploring the regulation of Rg1 on DFUs treatment and the underlying mechanism. Methods: Human umbilical vein endothelial cells (HUVECs) incubated with high-glucose culture medium were established for induction of diabetes model. The MTT assay, Annexin V/PI assay and oxidative stress detection were carried out on high-glucose-induced HUVECs. Dual-luciferase reporter assay was performed to prove the interaction of miR-489–3p and Sirt1. DFUs model was established to determine the efficiency of Rg1 and miR-489–3p in wound closure of DFUs in vivo. Results: Rg1 promoted cell proliferation, migration and angiogenesis, and reduced cell apoptosis in high-glucose-induced HUVECs. Knockdown of miR-489–3p alleviated the high-glucose-induced damage to HUVECs, while overexpression of miR-489–3p attenuated the protection effects of Rg1. Overexpression Sirt1 promoted wound healing in DFUs and Sirt1 was a direct target of miR-489–3p. In addition, animal experiments demonstrated that Rg1 promoted wound closure by regulating miR-489–3p/Sirt1 axis. Conclusions: Rg1 alleviated the DFUs by increasing Sirt1 expression via miR-489–3p downregulation and promoting activation of PI3K/AKT/eNOS signaling. |
| first_indexed | 2024-12-17T19:15:18Z |
| format | Article |
| id | doaj.art-489a347240fc49d8ada9813ee4abd55a |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-17T19:15:18Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-489a347240fc49d8ada9813ee4abd55a2022-12-21T21:35:46ZengElsevierJournal of Pharmacological Sciences1347-86132021-11-011473271283Ginsenoside Rg1 promoted the wound healing in diabetic foot ulcers via miR-489–3p/Sirt1 axisLiang Huang0Hua-An Cai1Ming-Sheng Zhang2Ruo-Yi Liao3Xing Huang4Feng-Dan Hu5Second Clinical Medical College, Southern Medical University, Guangzhou, 510515, Guangdong Province, PR China; Department of Rehabilitation, Guangdong Provincial People's Hospital, Guangzhou, 510515, Guangdong Province, PR ChinaDepartment of Rehabilitation Medicine, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410016, Hunan Province, PR China; Department of Sports Medicine, Institute of Translational Medicine, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410016, Hunan Province, PR China; Corresponding author. Department of Rehabilitation Medicine, Department of Sports Medicine, Institute of Translational Medicine, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, No. 89 Guhan Road, Changsha, 410016, Hunan Province, PR China.Second Clinical Medical College, Southern Medical University, Guangzhou, 510515, Guangdong Province, PR China; Department of Rehabilitation, Guangdong Provincial People's Hospital, Guangzhou, 510515, Guangdong Province, PR China; Corresponding author. Second Clinical Medical College, Southern Medical University; Department of Rehabilitation, Guangdong Provincial People's Hospital, No.1838 Guangzhou Avenue North, Guangzhou, 510515, Guangdong Province, PR China.First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha, 410004, Hunan Province, PR ChinaDepartment of General Surgery, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410016, Hunan Province, PR ChinaDepartment of Rehabilitation Medicine, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410016, Hunan Province, PR ChinaPurpose: Diabetic foot ulcers (DFUs) are common complications of high severity for diabetes. Ginsenoside Rg1 (Rg1) has the potential for diabetes and cardiovascular diseases therapy. This research aimed at exploring the regulation of Rg1 on DFUs treatment and the underlying mechanism. Methods: Human umbilical vein endothelial cells (HUVECs) incubated with high-glucose culture medium were established for induction of diabetes model. The MTT assay, Annexin V/PI assay and oxidative stress detection were carried out on high-glucose-induced HUVECs. Dual-luciferase reporter assay was performed to prove the interaction of miR-489–3p and Sirt1. DFUs model was established to determine the efficiency of Rg1 and miR-489–3p in wound closure of DFUs in vivo. Results: Rg1 promoted cell proliferation, migration and angiogenesis, and reduced cell apoptosis in high-glucose-induced HUVECs. Knockdown of miR-489–3p alleviated the high-glucose-induced damage to HUVECs, while overexpression of miR-489–3p attenuated the protection effects of Rg1. Overexpression Sirt1 promoted wound healing in DFUs and Sirt1 was a direct target of miR-489–3p. In addition, animal experiments demonstrated that Rg1 promoted wound closure by regulating miR-489–3p/Sirt1 axis. Conclusions: Rg1 alleviated the DFUs by increasing Sirt1 expression via miR-489–3p downregulation and promoting activation of PI3K/AKT/eNOS signaling.http://www.sciencedirect.com/science/article/pii/S134786132100075XDiabetic foot ulcersGinsenoside Rg1miR-489–3pSirt1Angiogenesis |
| spellingShingle | Liang Huang Hua-An Cai Ming-Sheng Zhang Ruo-Yi Liao Xing Huang Feng-Dan Hu Ginsenoside Rg1 promoted the wound healing in diabetic foot ulcers via miR-489–3p/Sirt1 axis Journal of Pharmacological Sciences Diabetic foot ulcers Ginsenoside Rg1 miR-489–3p Sirt1 Angiogenesis |
| title | Ginsenoside Rg1 promoted the wound healing in diabetic foot ulcers via miR-489–3p/Sirt1 axis |
| title_full | Ginsenoside Rg1 promoted the wound healing in diabetic foot ulcers via miR-489–3p/Sirt1 axis |
| title_fullStr | Ginsenoside Rg1 promoted the wound healing in diabetic foot ulcers via miR-489–3p/Sirt1 axis |
| title_full_unstemmed | Ginsenoside Rg1 promoted the wound healing in diabetic foot ulcers via miR-489–3p/Sirt1 axis |
| title_short | Ginsenoside Rg1 promoted the wound healing in diabetic foot ulcers via miR-489–3p/Sirt1 axis |
| title_sort | ginsenoside rg1 promoted the wound healing in diabetic foot ulcers via mir 489 3p sirt1 axis |
| topic | Diabetic foot ulcers Ginsenoside Rg1 miR-489–3p Sirt1 Angiogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S134786132100075X |
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