Molecular detection and characterization of the Mycobacterium tuberculosis complex subspecies responsible for bovine tuberculosis in Punjab, Pakistan
ABSTRACTBovine tuberculosis (bTB), traditionally associated with Mycobacterium bovis, presents significant public health and economic challenges worldwide. This study investigated the causative agents of bTB in slaughtered cattle and buffalo in Lahore, Pakistan. Of the 3,581 animals screened, 34 wer...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2024-02-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02692-23 |
| _version_ | 1797322755800039424 |
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| author | Rubab Maqsood Shannon C. Duffy Hamad Bin Rashid Shakera Sadiq Gill Chanda Jabeen Nimra Arshad Gulshan Umbreen Marcel A. Behr Vivek Kapur Mamoona Chaudhry |
| author_facet | Rubab Maqsood Shannon C. Duffy Hamad Bin Rashid Shakera Sadiq Gill Chanda Jabeen Nimra Arshad Gulshan Umbreen Marcel A. Behr Vivek Kapur Mamoona Chaudhry |
| author_sort | Rubab Maqsood |
| collection | DOAJ |
| description | ABSTRACTBovine tuberculosis (bTB), traditionally associated with Mycobacterium bovis, presents significant public health and economic challenges worldwide. This study investigated the causative agents of bTB in slaughtered cattle and buffalo in Lahore, Pakistan. Of the 3,581 animals screened, 34 were identified with gross TB-like lesions. The lesions were processed for culture, PCR, and Sanger sequencing to identify the causative agents of the disease. The results identified 10 Mycobacterium orygis and 8 Mycobacterium tuberculosis sensu stricto isolates. Whole-genome sequencing was performed on two M. orygis isolates, and the sequences were phylogenetically compared to 93 publicly available M. orygis sequences. The results also demonstrated that the JB21 and JB22 primers, which have been previously commonly applied to detect M. bovis in Pakistan, are unable to distinguish between M. tuberculosis complex subspecies. The identification of M. orygis and M. tuberculosis as causative agents of bTB in this slaughterhouse in Punjab may have important implications in identifying cases of zoonotic TB in humans and applying appropriate molecular tools to identify the prevalence of the disease. The data from this study align with recent findings suggesting M. orygis is the predominant cause of bTB in South Asia.IMPORTANCEThe study findings hold significant relevance to the Journal of Clinical Microbiology, as they directly impact the field. The first-time identification of Mycobacterium orygis and Mycobacterium tuberculosis as the predominant causative agents of bovine tuberculosis in Lahore, Pakistan underscores the urgent need for enhanced diagnostic methods. The study emphasizes the importance of improved assays for the accurate detection and differentiation of Mycobacterium subspecies. Additionally, the research addresses zoonotic risk assessment and public health implications, advocating for a multidisciplinary approach that integrates clinical microbiology with veterinary and human health sectors. These insights contribute to clinical microbiology knowledge, shaping effective strategies for disease prevention, surveillance, and control. The study’s potential to advance the field makes it well suited for publication in the Microbiology Spectrum journal. |
| first_indexed | 2024-03-08T05:18:54Z |
| format | Article |
| id | doaj.art-489bf0caa5184aa3af13b595e90b395c |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-08T05:18:54Z |
| publishDate | 2024-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-489bf0caa5184aa3af13b595e90b395c2024-02-06T14:04:55ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972024-02-0112210.1128/spectrum.02692-23Molecular detection and characterization of the Mycobacterium tuberculosis complex subspecies responsible for bovine tuberculosis in Punjab, PakistanRubab Maqsood0Shannon C. Duffy1Hamad Bin Rashid2Shakera Sadiq Gill3Chanda Jabeen4Nimra Arshad5Gulshan Umbreen6Marcel A. Behr7Vivek Kapur8Mamoona Chaudhry9Department of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, PakistanDepartment of Microbiology and Immunology, McGill University, Montreal, Quebec, CanadaDepartment of Veterinary Surgery and Pet Sciences, University of Veterinary and Animal Sciences, Lahore, PakistanDepartment of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, PakistanDepartment of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, PakistanDepartment of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, PakistanDepartment of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, PakistanDepartment of Microbiology and Immunology, McGill University, Montreal, Quebec, CanadaDepartment of Animal Science, The Pennsylvania State University, University Park, Pennsylvania, USADepartment of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, PakistanABSTRACTBovine tuberculosis (bTB), traditionally associated with Mycobacterium bovis, presents significant public health and economic challenges worldwide. This study investigated the causative agents of bTB in slaughtered cattle and buffalo in Lahore, Pakistan. Of the 3,581 animals screened, 34 were identified with gross TB-like lesions. The lesions were processed for culture, PCR, and Sanger sequencing to identify the causative agents of the disease. The results identified 10 Mycobacterium orygis and 8 Mycobacterium tuberculosis sensu stricto isolates. Whole-genome sequencing was performed on two M. orygis isolates, and the sequences were phylogenetically compared to 93 publicly available M. orygis sequences. The results also demonstrated that the JB21 and JB22 primers, which have been previously commonly applied to detect M. bovis in Pakistan, are unable to distinguish between M. tuberculosis complex subspecies. The identification of M. orygis and M. tuberculosis as causative agents of bTB in this slaughterhouse in Punjab may have important implications in identifying cases of zoonotic TB in humans and applying appropriate molecular tools to identify the prevalence of the disease. The data from this study align with recent findings suggesting M. orygis is the predominant cause of bTB in South Asia.IMPORTANCEThe study findings hold significant relevance to the Journal of Clinical Microbiology, as they directly impact the field. The first-time identification of Mycobacterium orygis and Mycobacterium tuberculosis as the predominant causative agents of bovine tuberculosis in Lahore, Pakistan underscores the urgent need for enhanced diagnostic methods. The study emphasizes the importance of improved assays for the accurate detection and differentiation of Mycobacterium subspecies. Additionally, the research addresses zoonotic risk assessment and public health implications, advocating for a multidisciplinary approach that integrates clinical microbiology with veterinary and human health sectors. These insights contribute to clinical microbiology knowledge, shaping effective strategies for disease prevention, surveillance, and control. The study’s potential to advance the field makes it well suited for publication in the Microbiology Spectrum journal.https://journals.asm.org/doi/10.1128/spectrum.02692-23bovine TBM. orygisM. tuberculosisslaughterhousemolecular identificationwhole-genome sequencing |
| spellingShingle | Rubab Maqsood Shannon C. Duffy Hamad Bin Rashid Shakera Sadiq Gill Chanda Jabeen Nimra Arshad Gulshan Umbreen Marcel A. Behr Vivek Kapur Mamoona Chaudhry Molecular detection and characterization of the Mycobacterium tuberculosis complex subspecies responsible for bovine tuberculosis in Punjab, Pakistan Microbiology Spectrum bovine TB M. orygis M. tuberculosis slaughterhouse molecular identification whole-genome sequencing |
| title | Molecular detection and characterization of the Mycobacterium tuberculosis complex subspecies responsible for bovine tuberculosis in Punjab, Pakistan |
| title_full | Molecular detection and characterization of the Mycobacterium tuberculosis complex subspecies responsible for bovine tuberculosis in Punjab, Pakistan |
| title_fullStr | Molecular detection and characterization of the Mycobacterium tuberculosis complex subspecies responsible for bovine tuberculosis in Punjab, Pakistan |
| title_full_unstemmed | Molecular detection and characterization of the Mycobacterium tuberculosis complex subspecies responsible for bovine tuberculosis in Punjab, Pakistan |
| title_short | Molecular detection and characterization of the Mycobacterium tuberculosis complex subspecies responsible for bovine tuberculosis in Punjab, Pakistan |
| title_sort | molecular detection and characterization of the mycobacterium tuberculosis complex subspecies responsible for bovine tuberculosis in punjab pakistan |
| topic | bovine TB M. orygis M. tuberculosis slaughterhouse molecular identification whole-genome sequencing |
| url | https://journals.asm.org/doi/10.1128/spectrum.02692-23 |
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