Epimicrobiome Shifts With Bleaching Disease Progression in the Brown Seaweed Saccharina japonica
Recent bacterial induced bleaching disease events of the commercially farmed brown seaweed Saccharina japonica has resulted in significant reduction in healthy sporeling supply. However, to date the host associated epimicrobial community shifts with the disease progression have not been characterize...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.865224/full |
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| author | Fan Ling Fan Ling Suhelen Egan Yingrui Zhuang Yingrui Zhuang Lirong Chang Luyang Xiao Qin Yang Qin Yang Gaoge Wang Gaoge Wang |
| author_facet | Fan Ling Fan Ling Suhelen Egan Yingrui Zhuang Yingrui Zhuang Lirong Chang Luyang Xiao Qin Yang Qin Yang Gaoge Wang Gaoge Wang |
| author_sort | Fan Ling |
| collection | DOAJ |
| description | Recent bacterial induced bleaching disease events of the commercially farmed brown seaweed Saccharina japonica has resulted in significant reduction in healthy sporeling supply. However, to date the host associated epimicrobial community shifts with the disease progression have not been characterized. We investigated the dynamic shifts in the composition of the epibacterial communities of S. japonica with disease progression using 16S rRNA gene amplicon sequencing. We found that the alpha diversity was significantly higher in the control group than in the infected group over disease progression (P < 0.01). There was a significant shift in the composition and predicted functions of the epibacterial communities in both control and infected groups. Indicator species, belonging to Stenotrophomonas, Pseudomonas and Sphingomona were significantly higher in the control group than infected group, suggesting that these taxa are associated with healthy S. japonica. In contrast, Vibrio, Pseudoalteromonas and Colwellia were keystone taxa in the infected group at 24 h, and thus maybe considered to be the secondary opportunistic pathogens. Our study describes the changes of epibacterial communities associated with the progression of bleaching disease in S. japonica. This new information not only extends our baseline knowledge of the S. japonica epimicrobiome, but also paves the way for developing measures to mitigate disease outbreaks for the sustainable aquaculture of S. japonica. |
| first_indexed | 2024-04-14T01:15:44Z |
| format | Article |
| id | doaj.art-489bff5c2c3d4618b0e865ac95a4c5fd |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-14T01:15:44Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-489bff5c2c3d4618b0e865ac95a4c5fd2022-12-22T02:20:52ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-04-01910.3389/fmars.2022.865224865224Epimicrobiome Shifts With Bleaching Disease Progression in the Brown Seaweed Saccharina japonicaFan Ling0Fan Ling1Suhelen Egan2Yingrui Zhuang3Yingrui Zhuang4Lirong Chang5Luyang Xiao6Qin Yang7Qin Yang8Gaoge Wang9Gaoge Wang10College of Marine Life Sciences, Ocean University of China, Qingdao, ChinaInstitute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaCentre for Marine Science and Innovation and School of Biological, Earth and Environmental Sciences, The University of New South Wales, Sydney, NSW, AustraliaCollege of Marine Life Sciences, Ocean University of China, Qingdao, ChinaInstitute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaWeihai Changqing Ocean Science and Technology Co., Ltd, Rongcheng, ChinaWeihai Changqing Ocean Science and Technology Co., Ltd, Rongcheng, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao, ChinaInstitute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao, ChinaInstitute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, ChinaRecent bacterial induced bleaching disease events of the commercially farmed brown seaweed Saccharina japonica has resulted in significant reduction in healthy sporeling supply. However, to date the host associated epimicrobial community shifts with the disease progression have not been characterized. We investigated the dynamic shifts in the composition of the epibacterial communities of S. japonica with disease progression using 16S rRNA gene amplicon sequencing. We found that the alpha diversity was significantly higher in the control group than in the infected group over disease progression (P < 0.01). There was a significant shift in the composition and predicted functions of the epibacterial communities in both control and infected groups. Indicator species, belonging to Stenotrophomonas, Pseudomonas and Sphingomona were significantly higher in the control group than infected group, suggesting that these taxa are associated with healthy S. japonica. In contrast, Vibrio, Pseudoalteromonas and Colwellia were keystone taxa in the infected group at 24 h, and thus maybe considered to be the secondary opportunistic pathogens. Our study describes the changes of epibacterial communities associated with the progression of bleaching disease in S. japonica. This new information not only extends our baseline knowledge of the S. japonica epimicrobiome, but also paves the way for developing measures to mitigate disease outbreaks for the sustainable aquaculture of S. japonica.https://www.frontiersin.org/articles/10.3389/fmars.2022.865224/fullbleaching diseaseepimicrobiome shiftspathogenic bacteria16S rRNA gene amplicon sequencingSaccharina japonica |
| spellingShingle | Fan Ling Fan Ling Suhelen Egan Yingrui Zhuang Yingrui Zhuang Lirong Chang Luyang Xiao Qin Yang Qin Yang Gaoge Wang Gaoge Wang Epimicrobiome Shifts With Bleaching Disease Progression in the Brown Seaweed Saccharina japonica Frontiers in Marine Science bleaching disease epimicrobiome shifts pathogenic bacteria 16S rRNA gene amplicon sequencing Saccharina japonica |
| title | Epimicrobiome Shifts With Bleaching Disease Progression in the Brown Seaweed Saccharina japonica |
| title_full | Epimicrobiome Shifts With Bleaching Disease Progression in the Brown Seaweed Saccharina japonica |
| title_fullStr | Epimicrobiome Shifts With Bleaching Disease Progression in the Brown Seaweed Saccharina japonica |
| title_full_unstemmed | Epimicrobiome Shifts With Bleaching Disease Progression in the Brown Seaweed Saccharina japonica |
| title_short | Epimicrobiome Shifts With Bleaching Disease Progression in the Brown Seaweed Saccharina japonica |
| title_sort | epimicrobiome shifts with bleaching disease progression in the brown seaweed saccharina japonica |
| topic | bleaching disease epimicrobiome shifts pathogenic bacteria 16S rRNA gene amplicon sequencing Saccharina japonica |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.865224/full |
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