Expression of Beta-Catenin in Colorectal Carcinoma and its Association with Various Clinicopathological Parameters
Introduction: Colorectal Carcinoma (CRC) is the third most commonly diagnosed cancer and the second leading cause of cancer related mortality globally. It is a source of concern for researchers worldwide and hence, a lot of emphasis is being given towards early detection and targeted drug therap...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2021-07-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/15151/48129_CE[Ra1]_F[IK]_PF1(PS_SHU)_PFA(PS_KM)_PN(KM).pdf |
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| author | Monika Panda Ranjita Panigrahi Goutami Das Nayak Urmila Senapati Saroj Ranjan Sahoo Ipsa Mohapatra |
| author_facet | Monika Panda Ranjita Panigrahi Goutami Das Nayak Urmila Senapati Saroj Ranjan Sahoo Ipsa Mohapatra |
| author_sort | Monika Panda |
| collection | DOAJ |
| description | Introduction: Colorectal Carcinoma (CRC) is the third most
commonly diagnosed cancer and the second leading cause of
cancer related mortality globally. It is a source of concern for
researchers worldwide and hence, a lot of emphasis is being
given towards early detection and targeted drug therapy to
improve the survival rate.
Aim: To study the expression of beta-catenin in colonic polyps,
adenomas and CRC and to associate beta-catenin expression
with various clinicopathological features.
Materials and Methods: This was a prospective cross-sectional
study conducted in the Department of Pathology, Kalinga Institute
of Medical Sciences, Bhubaneswar, Odisha, India from September
2018 to August 2020. Colonoscopic biopsies, mucinous carcinoma
and poorly preserved tissue were excluded. Histopathological
study and Immunohistochemistry evaluation of beta-catenin was
done. Statistical analysis was done by using appropriate tests. A
p-value of less than 0.05 was taken as statistically significant.
Results: Out of 80 cases, 40 cases were benign lesions
Non neoplastic polyp and adenoma) and 40 cases were
adenocarcinoma. It was observed that benign lesions had
maximum cases with preserved membranous expression (36/40)
and very few cases (4/40) showed cytoplasmic expression of betacatenin. But in carcinoma, high cytoplasmic expression was seen
in 20/40 (50%) whereas 8/40 (20%) cases had nuclear positivity.
Membranous beta-catenin expression was significantly higher in
benign lesions than in the malignant lesions (IS:8.75±3.09 versus
4.30±2.70) respectively; (p<0.0001). But cytoplasmic beta-catenin
expression was low in benign lesion as compared to malignant
lesion (IS: 2.07±3.46 versus 5.35±3.14), respectively; (p<0.0001).
However, nuclear beta-catenin expression was extremely low
in benign lesions than in malignant lesions (0.08±0.47 versus
1.90±3.49), respectively; (p=0.0016), this difference was statistically
significant.
Conclusion: The present study demonstrates the change in betacatenin expression with gradual transition from predominantly
membranous pattern to cytoplasmic or nuclear as we progress
from normal colorectal tissues to polyps, benign premalignant
lesions and malignant neoplasms. This property of beta-catenin
helps in determining malignant potential of various premalignant
neoplasms of large intestine which in turn helps in initiating early
prophylactic treatment. |
| first_indexed | 2024-12-20T18:13:36Z |
| format | Article |
| id | doaj.art-48a0872d85a64bf89f403c3acacf7740 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-20T18:13:36Z |
| publishDate | 2021-07-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-48a0872d85a64bf89f403c3acacf77402022-12-21T19:30:26ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2021-07-011507303410.7860/JCDR/2021/48129.15151Expression of Beta-Catenin in Colorectal Carcinoma and its Association with Various Clinicopathological ParametersMonika Panda0Ranjita Panigrahi1Goutami Das Nayak2Urmila Senapati3Saroj Ranjan Sahoo4 Ipsa Mohapatra5Postgraduate Student, Department of Pathology, Kalinga Institute of Medical Sciences, KIIT University, Bhubaneswar, Odisha, IndiaProfessor, Department of Pathology, Kalinga Institute of Medical Sciences, KIIT University, Bhubaneswar, Odisha, IndiaAssistant Professor, Department of Pathology, Kalinga Institute of Medical Sciences, KIIT University, Bhubaneswar, Odisha, IndiaProfessor and Head of the Department, Department of Pathology, Kalinga Institute of Medical Sciences, KIIT University, Bhubaneswar, Odisha, IndiaAssociate Professor, Department of Surgical Oncology, Kalinga Institute of Medical Sciences, KIIT University, Bhubaneswar, Odisha, IndiaAssociate Professor, Department of Social and Preventive Medicine, Kalinga Institute of Medical Sciences, KIIT University, Bhubaneswar, Odisha, IndiaIntroduction: Colorectal Carcinoma (CRC) is the third most commonly diagnosed cancer and the second leading cause of cancer related mortality globally. It is a source of concern for researchers worldwide and hence, a lot of emphasis is being given towards early detection and targeted drug therapy to improve the survival rate. Aim: To study the expression of beta-catenin in colonic polyps, adenomas and CRC and to associate beta-catenin expression with various clinicopathological features. Materials and Methods: This was a prospective cross-sectional study conducted in the Department of Pathology, Kalinga Institute of Medical Sciences, Bhubaneswar, Odisha, India from September 2018 to August 2020. Colonoscopic biopsies, mucinous carcinoma and poorly preserved tissue were excluded. Histopathological study and Immunohistochemistry evaluation of beta-catenin was done. Statistical analysis was done by using appropriate tests. A p-value of less than 0.05 was taken as statistically significant. Results: Out of 80 cases, 40 cases were benign lesions Non neoplastic polyp and adenoma) and 40 cases were adenocarcinoma. It was observed that benign lesions had maximum cases with preserved membranous expression (36/40) and very few cases (4/40) showed cytoplasmic expression of betacatenin. But in carcinoma, high cytoplasmic expression was seen in 20/40 (50%) whereas 8/40 (20%) cases had nuclear positivity. Membranous beta-catenin expression was significantly higher in benign lesions than in the malignant lesions (IS:8.75±3.09 versus 4.30±2.70) respectively; (p<0.0001). But cytoplasmic beta-catenin expression was low in benign lesion as compared to malignant lesion (IS: 2.07±3.46 versus 5.35±3.14), respectively; (p<0.0001). However, nuclear beta-catenin expression was extremely low in benign lesions than in malignant lesions (0.08±0.47 versus 1.90±3.49), respectively; (p=0.0016), this difference was statistically significant. Conclusion: The present study demonstrates the change in betacatenin expression with gradual transition from predominantly membranous pattern to cytoplasmic or nuclear as we progress from normal colorectal tissues to polyps, benign premalignant lesions and malignant neoplasms. This property of beta-catenin helps in determining malignant potential of various premalignant neoplasms of large intestine which in turn helps in initiating early prophylactic treatment.https://jcdr.net/articles/PDF/15151/48129_CE[Ra1]_F[IK]_PF1(PS_SHU)_PFA(PS_KM)_PN(KM).pdfadenomatous polypcolorectal neoplasmsmembranous expression |
| spellingShingle | Monika Panda Ranjita Panigrahi Goutami Das Nayak Urmila Senapati Saroj Ranjan Sahoo Ipsa Mohapatra Expression of Beta-Catenin in Colorectal Carcinoma and its Association with Various Clinicopathological Parameters Journal of Clinical and Diagnostic Research adenomatous polyp colorectal neoplasms membranous expression |
| title | Expression of Beta-Catenin in Colorectal Carcinoma and its Association with Various Clinicopathological Parameters |
| title_full | Expression of Beta-Catenin in Colorectal Carcinoma and its Association with Various Clinicopathological Parameters |
| title_fullStr | Expression of Beta-Catenin in Colorectal Carcinoma and its Association with Various Clinicopathological Parameters |
| title_full_unstemmed | Expression of Beta-Catenin in Colorectal Carcinoma and its Association with Various Clinicopathological Parameters |
| title_short | Expression of Beta-Catenin in Colorectal Carcinoma and its Association with Various Clinicopathological Parameters |
| title_sort | expression of beta catenin in colorectal carcinoma and its association with various clinicopathological parameters |
| topic | adenomatous polyp colorectal neoplasms membranous expression |
| url | https://jcdr.net/articles/PDF/15151/48129_CE[Ra1]_F[IK]_PF1(PS_SHU)_PFA(PS_KM)_PN(KM).pdf |
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