Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets.
The intestinal upper villus epithelial cells represent the differentiated epithelial cells and play key role in digesting and absorbing lumenal nutrients. Weaning stress commonly results in a decrease in villus height and intestinal dysfunction in piglets. However, no study have been conducted to te...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4811545?pdf=render |
| _version_ | 1818175845594824704 |
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| author | Huansheng Yang Xia Xiong Xiaocheng Wang Bie Tan Tiejun Li Yulong Yin |
| author_facet | Huansheng Yang Xia Xiong Xiaocheng Wang Bie Tan Tiejun Li Yulong Yin |
| author_sort | Huansheng Yang |
| collection | DOAJ |
| description | The intestinal upper villus epithelial cells represent the differentiated epithelial cells and play key role in digesting and absorbing lumenal nutrients. Weaning stress commonly results in a decrease in villus height and intestinal dysfunction in piglets. However, no study have been conducted to test the effects of weaning on the physiology and functions of upper villus epithelial cells. A total of 40 piglets from 8 litters were weaned at 14 days of age and one piglet from each litter was killed at 0 d (w0d), 1 d (w1d), 3 d (w3d), 5 d (w5d), and 7 d (w7d) after weaning, respectively. The upper villus epithelial cells in mid-jejunum were isolated using the distended intestinal sac method. The expression of proteins in upper villus epithelial cells was analyzed using the isobaric tags for relative and absolute quantification or Western blotting. The expression of proteins involved in energy metabolism, Golgi vesicle transport, protein amino acid glycosylation, secretion by cell, transmembrane transport, ion transport, nucleotide catabolic process, translational initiation, and epithelial cell differentiation and apoptosis, was mainly reduced during the post-weaning period, and these processes may be regulated by mTOR signaling pathway. These results indicated that weaning inhibited various cellular processes in jejunal upper villus epithelial cells, and provided potential new directions for exploring the effects of weaning on the functions of intestine and improving intestinal functions in weaning piglets. |
| first_indexed | 2024-12-11T20:06:46Z |
| format | Article |
| id | doaj.art-48a12e46221b4079bea761b2ff3da516 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T20:06:46Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-48a12e46221b4079bea761b2ff3da5162022-12-22T00:52:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015021610.1371/journal.pone.0150216Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets.Huansheng YangXia XiongXiaocheng WangBie TanTiejun LiYulong YinThe intestinal upper villus epithelial cells represent the differentiated epithelial cells and play key role in digesting and absorbing lumenal nutrients. Weaning stress commonly results in a decrease in villus height and intestinal dysfunction in piglets. However, no study have been conducted to test the effects of weaning on the physiology and functions of upper villus epithelial cells. A total of 40 piglets from 8 litters were weaned at 14 days of age and one piglet from each litter was killed at 0 d (w0d), 1 d (w1d), 3 d (w3d), 5 d (w5d), and 7 d (w7d) after weaning, respectively. The upper villus epithelial cells in mid-jejunum were isolated using the distended intestinal sac method. The expression of proteins in upper villus epithelial cells was analyzed using the isobaric tags for relative and absolute quantification or Western blotting. The expression of proteins involved in energy metabolism, Golgi vesicle transport, protein amino acid glycosylation, secretion by cell, transmembrane transport, ion transport, nucleotide catabolic process, translational initiation, and epithelial cell differentiation and apoptosis, was mainly reduced during the post-weaning period, and these processes may be regulated by mTOR signaling pathway. These results indicated that weaning inhibited various cellular processes in jejunal upper villus epithelial cells, and provided potential new directions for exploring the effects of weaning on the functions of intestine and improving intestinal functions in weaning piglets.http://europepmc.org/articles/PMC4811545?pdf=render |
| spellingShingle | Huansheng Yang Xia Xiong Xiaocheng Wang Bie Tan Tiejun Li Yulong Yin Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets. PLoS ONE |
| title | Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets. |
| title_full | Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets. |
| title_fullStr | Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets. |
| title_full_unstemmed | Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets. |
| title_short | Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets. |
| title_sort | effects of weaning on intestinal upper villus epithelial cells of piglets |
| url | http://europepmc.org/articles/PMC4811545?pdf=render |
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