Monoclonal Antibodies against Nucleocapsid Protein of SARS-CoV-2 Variants for Detection of COVID-19
Mitigation strategies of the coronavirus disease 2019 (COVID-19) pandemic have been greatly hindered by the continuous emergence of SARS-CoV-2 variants. New sensitive, rapid diagnostic tests for the wide-spectrum detection of viral variants are needed. We generated a panel of 41 monoclonal antibodie...
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/22/12412 |
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| author | Ruei-Min Lu Shih-Han Ko Wan-Yu Chen Yu-Ling Chang Hsiu-Ting Lin Han-Chung Wu |
| author_facet | Ruei-Min Lu Shih-Han Ko Wan-Yu Chen Yu-Ling Chang Hsiu-Ting Lin Han-Chung Wu |
| author_sort | Ruei-Min Lu |
| collection | DOAJ |
| description | Mitigation strategies of the coronavirus disease 2019 (COVID-19) pandemic have been greatly hindered by the continuous emergence of SARS-CoV-2 variants. New sensitive, rapid diagnostic tests for the wide-spectrum detection of viral variants are needed. We generated a panel of 41 monoclonal antibodies against the SARS-CoV-2 nucleocapsid protein (NP) by using mice hybridoma techniques. Of these mAbs, nine exhibited high binding activities and were applied in latex-based lateral flow immunoassays (LFIAs). The LFIAs utilizing NP-mAb-7 and -40 had the best sensitivity and lowest limit of detection: 8 pg for purified NP and 625 TCID<sub>50</sub>/mL for the authentic virus (hCoV-19/Taiwan/4/2020). The specificity tests showed that the NP-mAb-40/7 LFIA strips did not cross-react with five human coronavirus strains or 20 other common respiratory pathogens. Importantly, we found that 10 NP mutants, including alpha (B.1.1.7), beta (B.1.351), gamma (P.1), and delta (B.1.617.2) variants, could be detected by NP-mAb-40/7 LFIA strips. A clinical study (<i>n</i> = 60) of the NP-mAb-40/7 LFIA strips demonstrated a specificity of 100% and sensitivity of 90% in infected individuals with cycle threshold (Ct) values < 29.5. These anti-NP mAbs have strong potential for use in the clinical detection of SARS-CoV-2 infection, whether the virus is wild-type or a variant of concern. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T05:25:30Z |
| publishDate | 2021-11-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-48a723907e214b449fcf2ffaf9e493f82023-11-22T23:41:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221241210.3390/ijms222212412Monoclonal Antibodies against Nucleocapsid Protein of SARS-CoV-2 Variants for Detection of COVID-19Ruei-Min Lu0Shih-Han Ko1Wan-Yu Chen2Yu-Ling Chang3Hsiu-Ting Lin4Han-Chung Wu5Biomedical Translation Research Center (BioTReC), Academia Sinica, Taipei 11529, TaiwanBiomedical Translation Research Center (BioTReC), Academia Sinica, Taipei 11529, TaiwanInstitute of Cellular and Organismic Biology, Academia Sinica, Taipei 11529, TaiwanBiomedical Translation Research Center (BioTReC), Academia Sinica, Taipei 11529, TaiwanInstitute of Cellular and Organismic Biology, Academia Sinica, Taipei 11529, TaiwanBiomedical Translation Research Center (BioTReC), Academia Sinica, Taipei 11529, TaiwanMitigation strategies of the coronavirus disease 2019 (COVID-19) pandemic have been greatly hindered by the continuous emergence of SARS-CoV-2 variants. New sensitive, rapid diagnostic tests for the wide-spectrum detection of viral variants are needed. We generated a panel of 41 monoclonal antibodies against the SARS-CoV-2 nucleocapsid protein (NP) by using mice hybridoma techniques. Of these mAbs, nine exhibited high binding activities and were applied in latex-based lateral flow immunoassays (LFIAs). The LFIAs utilizing NP-mAb-7 and -40 had the best sensitivity and lowest limit of detection: 8 pg for purified NP and 625 TCID<sub>50</sub>/mL for the authentic virus (hCoV-19/Taiwan/4/2020). The specificity tests showed that the NP-mAb-40/7 LFIA strips did not cross-react with five human coronavirus strains or 20 other common respiratory pathogens. Importantly, we found that 10 NP mutants, including alpha (B.1.1.7), beta (B.1.351), gamma (P.1), and delta (B.1.617.2) variants, could be detected by NP-mAb-40/7 LFIA strips. A clinical study (<i>n</i> = 60) of the NP-mAb-40/7 LFIA strips demonstrated a specificity of 100% and sensitivity of 90% in infected individuals with cycle threshold (Ct) values < 29.5. These anti-NP mAbs have strong potential for use in the clinical detection of SARS-CoV-2 infection, whether the virus is wild-type or a variant of concern.https://www.mdpi.com/1422-0067/22/22/12412COVID-19SARS-CoV-2antibodynucleocapsid proteinrapid testLFIA |
| spellingShingle | Ruei-Min Lu Shih-Han Ko Wan-Yu Chen Yu-Ling Chang Hsiu-Ting Lin Han-Chung Wu Monoclonal Antibodies against Nucleocapsid Protein of SARS-CoV-2 Variants for Detection of COVID-19 International Journal of Molecular Sciences COVID-19 SARS-CoV-2 antibody nucleocapsid protein rapid test LFIA |
| title | Monoclonal Antibodies against Nucleocapsid Protein of SARS-CoV-2 Variants for Detection of COVID-19 |
| title_full | Monoclonal Antibodies against Nucleocapsid Protein of SARS-CoV-2 Variants for Detection of COVID-19 |
| title_fullStr | Monoclonal Antibodies against Nucleocapsid Protein of SARS-CoV-2 Variants for Detection of COVID-19 |
| title_full_unstemmed | Monoclonal Antibodies against Nucleocapsid Protein of SARS-CoV-2 Variants for Detection of COVID-19 |
| title_short | Monoclonal Antibodies against Nucleocapsid Protein of SARS-CoV-2 Variants for Detection of COVID-19 |
| title_sort | monoclonal antibodies against nucleocapsid protein of sars cov 2 variants for detection of covid 19 |
| topic | COVID-19 SARS-CoV-2 antibody nucleocapsid protein rapid test LFIA |
| url | https://www.mdpi.com/1422-0067/22/22/12412 |
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