<i>Clostridium botulinum</i> and <i>Clostridium perfringens</i> Occurrence in Kazakh Honey Samples
The aim of this study was to assess occurrence of <i>Clostridium botulinum</i> and <i>Clostridium perfringens</i> in honey samples from Kazakhstan. Analyses were carried out using a set of PCR methods for identification of anaerobic bacteria, and detection of toxin genes of &...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-08-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/11/8/472 |
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| author | Balgabay Maikanov Raikhan Mustafina Laura Auteleyeva Jan Wiśniewski Krzysztof Anusz Tomasz Grenda Krzysztof Kwiatek Magdalena Goldsztejn Magdalena Grabczak |
| author_facet | Balgabay Maikanov Raikhan Mustafina Laura Auteleyeva Jan Wiśniewski Krzysztof Anusz Tomasz Grenda Krzysztof Kwiatek Magdalena Goldsztejn Magdalena Grabczak |
| author_sort | Balgabay Maikanov |
| collection | DOAJ |
| description | The aim of this study was to assess occurrence of <i>Clostridium botulinum</i> and <i>Clostridium perfringens</i> in honey samples from Kazakhstan. Analyses were carried out using a set of PCR methods for identification of anaerobic bacteria, and detection of toxin genes of <i>C. botulinum</i> and <i>C. perfringens</i>. Among 197 samples, <i>C. botulinum</i> was noticed in only one (0.5%). The isolated strain of this pathogen showed the presence of the <i>bont/A</i> and <i>ntnh</i> genes. <i>C. perfringens</i> strains were isolated from 18 (9%) samples, and mPCR (multiplex PCR) analysis led to them all being classified as toxin type A with the ability to produce α toxin. Sequence analysis of 16S rDNA genes showed occurrence in 4 samples of other anaerobes related to <i>C. botulinum</i>, which were <i>C. sporogenes</i> and <i>C. beijerinckii</i> strains. <i>C. botulinum</i> prevalence in honey samples from Kazakhstan in comparison to the prevalence in samples collected from the other regions seems to be less. The highest prevalence of <i>Clostridium</i> sp. was noticed in the East Kazakhstan province. Our study is the first survey on BoNT-producing clostridia and <i>C. perfringens</i> prevalence in Kazakh honey. |
| first_indexed | 2024-04-14T02:25:17Z |
| format | Article |
| id | doaj.art-48a7663c584d4ce5a8f54a46411a8e26 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T02:25:17Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-48a7663c584d4ce5a8f54a46411a8e262022-12-22T02:17:54ZengMDPI AGToxins2072-66512019-08-0111847210.3390/toxins11080472toxins11080472<i>Clostridium botulinum</i> and <i>Clostridium perfringens</i> Occurrence in Kazakh Honey SamplesBalgabay Maikanov0Raikhan Mustafina1Laura Auteleyeva2Jan Wiśniewski3Krzysztof Anusz4Tomasz Grenda5Krzysztof Kwiatek6Magdalena Goldsztejn7Magdalena Grabczak8Department of Veterinary Sanitation, Faculty of Veterinary Medicine and Technology of Animal Husbandry, S. Seifullin Kazakh Agro Technical University, Zhenis Avenue 62, 010011 Astana, Republic of KazakhstanDepartment of Veterinary Sanitation, Faculty of Veterinary Medicine and Technology of Animal Husbandry, S. Seifullin Kazakh Agro Technical University, Zhenis Avenue 62, 010011 Astana, Republic of KazakhstanDepartment of Veterinary Sanitation, Faculty of Veterinary Medicine and Technology of Animal Husbandry, S. Seifullin Kazakh Agro Technical University, Zhenis Avenue 62, 010011 Astana, Republic of KazakhstanDepartament of Food Hygiene and Public Health Protection, Faculty of Veterinary Medicine, Warsaw University of Life Sciences—SGGW (WULS-SGGW), ul.Nowoursynowska 159, 02-776 Warsaw, PolandDepartament of Food Hygiene and Public Health Protection, Faculty of Veterinary Medicine, Warsaw University of Life Sciences—SGGW (WULS-SGGW), ul.Nowoursynowska 159, 02-776 Warsaw, PolandDepartment of Hygiene of Animal Feeding Stuffs, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, PolandDepartment of Hygiene of Animal Feeding Stuffs, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, PolandDepartment of Hygiene of Animal Feeding Stuffs, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, PolandDepartment of Hygiene of Animal Feeding Stuffs, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, PolandThe aim of this study was to assess occurrence of <i>Clostridium botulinum</i> and <i>Clostridium perfringens</i> in honey samples from Kazakhstan. Analyses were carried out using a set of PCR methods for identification of anaerobic bacteria, and detection of toxin genes of <i>C. botulinum</i> and <i>C. perfringens</i>. Among 197 samples, <i>C. botulinum</i> was noticed in only one (0.5%). The isolated strain of this pathogen showed the presence of the <i>bont/A</i> and <i>ntnh</i> genes. <i>C. perfringens</i> strains were isolated from 18 (9%) samples, and mPCR (multiplex PCR) analysis led to them all being classified as toxin type A with the ability to produce α toxin. Sequence analysis of 16S rDNA genes showed occurrence in 4 samples of other anaerobes related to <i>C. botulinum</i>, which were <i>C. sporogenes</i> and <i>C. beijerinckii</i> strains. <i>C. botulinum</i> prevalence in honey samples from Kazakhstan in comparison to the prevalence in samples collected from the other regions seems to be less. The highest prevalence of <i>Clostridium</i> sp. was noticed in the East Kazakhstan province. Our study is the first survey on BoNT-producing clostridia and <i>C. perfringens</i> prevalence in Kazakh honey.https://www.mdpi.com/2072-6651/11/8/472<i>C. botulinum</i><i>C. perfringens</i>honeyKazakhstan |
| spellingShingle | Balgabay Maikanov Raikhan Mustafina Laura Auteleyeva Jan Wiśniewski Krzysztof Anusz Tomasz Grenda Krzysztof Kwiatek Magdalena Goldsztejn Magdalena Grabczak <i>Clostridium botulinum</i> and <i>Clostridium perfringens</i> Occurrence in Kazakh Honey Samples Toxins <i>C. botulinum</i> <i>C. perfringens</i> honey Kazakhstan |
| title | <i>Clostridium botulinum</i> and <i>Clostridium perfringens</i> Occurrence in Kazakh Honey Samples |
| title_full | <i>Clostridium botulinum</i> and <i>Clostridium perfringens</i> Occurrence in Kazakh Honey Samples |
| title_fullStr | <i>Clostridium botulinum</i> and <i>Clostridium perfringens</i> Occurrence in Kazakh Honey Samples |
| title_full_unstemmed | <i>Clostridium botulinum</i> and <i>Clostridium perfringens</i> Occurrence in Kazakh Honey Samples |
| title_short | <i>Clostridium botulinum</i> and <i>Clostridium perfringens</i> Occurrence in Kazakh Honey Samples |
| title_sort | i clostridium botulinum i and i clostridium perfringens i occurrence in kazakh honey samples |
| topic | <i>C. botulinum</i> <i>C. perfringens</i> honey Kazakhstan |
| url | https://www.mdpi.com/2072-6651/11/8/472 |
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