Buccal swabs for long-term DNA storage in conservation genetics of fish: One-and-a-half-year analysis timeframe
Conservation genetic research is essential for the management and recovery of endangered taxa. However, the invasive collection of biological material for DNA analysis is controversial. From an ethical perspective, non-destructive sampling methods leave the aquatic specimen alive and less invasive p...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
|
| Series: | Global Ecology and Conservation |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2351989424000714 |
| _version_ | 1797289730768896000 |
|---|---|
| author | Simona Sciuto Silvia Colussi Giuseppe Esposito Arianna Meletiadis Marino Prearo Elisabetta Pizzul Pier Luigi Acutis Rodolphe Elie Gozlan Paolo Pastorino |
| author_facet | Simona Sciuto Silvia Colussi Giuseppe Esposito Arianna Meletiadis Marino Prearo Elisabetta Pizzul Pier Luigi Acutis Rodolphe Elie Gozlan Paolo Pastorino |
| author_sort | Simona Sciuto |
| collection | DOAJ |
| description | Conservation genetic research is essential for the management and recovery of endangered taxa. However, the invasive collection of biological material for DNA analysis is controversial. From an ethical perspective, non-destructive sampling methods leave the aquatic specimen alive and less invasive procedures minimize stress on the animals. DNA can be obtained from fish using minimally invasive techniques such as buccal swabs. Here we evaluated the performance of buccal swabs for long-term storage of DNA obtained from brown trout (Salmo trutta). The buccal swabs were stored at room temperature and cut into pieces, one part of which was used for extraction of an aliquot and the others were stored as a “biobank” of biological material. The elapsed time from sampling to molecular analysis was one and half year. The amplification of three different DNA targets was tested to assess the effectiveness of the extraction: mitochondrial DNA (the D-LOOP region), nuclear DNA (the LDH gene) and microsatellite DNA at multiple loci. The results showed high quantification (mean value: 281.84±72.4 ng/µL), indicating that DNA could be effectively extracted from the buccal swabs. Our study results suggest that buccal swabs for long-term storage of DNA at room temperature are promising for use in field conservation studies. |
| first_indexed | 2024-03-07T19:09:13Z |
| format | Article |
| id | doaj.art-48a77a26d26a46699904563de2d5faad |
| institution | Directory Open Access Journal |
| issn | 2351-9894 |
| language | English |
| last_indexed | 2024-03-07T19:09:13Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Global Ecology and Conservation |
| spelling | doaj.art-48a77a26d26a46699904563de2d5faad2024-03-01T05:06:35ZengElsevierGlobal Ecology and Conservation2351-98942024-06-0151e02867Buccal swabs for long-term DNA storage in conservation genetics of fish: One-and-a-half-year analysis timeframeSimona Sciuto0Silvia Colussi1Giuseppe Esposito2Arianna Meletiadis3Marino Prearo4Elisabetta Pizzul5Pier Luigi Acutis6Rodolphe Elie Gozlan7Paolo Pastorino8Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino 10154, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino 10154, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino 10154, Italy; Corresponding author.Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino 10154, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino 10154, ItalyDipartimento di Scienze della Vita, Università degli Studi di Trieste, Trieste 34127, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino 10154, ItalyISEM, Université de Montpellier, CNRS, IRD, Montpellier 34095, FranceIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino 10154, ItalyConservation genetic research is essential for the management and recovery of endangered taxa. However, the invasive collection of biological material for DNA analysis is controversial. From an ethical perspective, non-destructive sampling methods leave the aquatic specimen alive and less invasive procedures minimize stress on the animals. DNA can be obtained from fish using minimally invasive techniques such as buccal swabs. Here we evaluated the performance of buccal swabs for long-term storage of DNA obtained from brown trout (Salmo trutta). The buccal swabs were stored at room temperature and cut into pieces, one part of which was used for extraction of an aliquot and the others were stored as a “biobank” of biological material. The elapsed time from sampling to molecular analysis was one and half year. The amplification of three different DNA targets was tested to assess the effectiveness of the extraction: mitochondrial DNA (the D-LOOP region), nuclear DNA (the LDH gene) and microsatellite DNA at multiple loci. The results showed high quantification (mean value: 281.84±72.4 ng/µL), indicating that DNA could be effectively extracted from the buccal swabs. Our study results suggest that buccal swabs for long-term storage of DNA at room temperature are promising for use in field conservation studies.http://www.sciencedirect.com/science/article/pii/S2351989424000714Conservation geneticsPrinciple of the 3RsDNA long-term storageAquatic speciesBiobank |
| spellingShingle | Simona Sciuto Silvia Colussi Giuseppe Esposito Arianna Meletiadis Marino Prearo Elisabetta Pizzul Pier Luigi Acutis Rodolphe Elie Gozlan Paolo Pastorino Buccal swabs for long-term DNA storage in conservation genetics of fish: One-and-a-half-year analysis timeframe Global Ecology and Conservation Conservation genetics Principle of the 3Rs DNA long-term storage Aquatic species Biobank |
| title | Buccal swabs for long-term DNA storage in conservation genetics of fish: One-and-a-half-year analysis timeframe |
| title_full | Buccal swabs for long-term DNA storage in conservation genetics of fish: One-and-a-half-year analysis timeframe |
| title_fullStr | Buccal swabs for long-term DNA storage in conservation genetics of fish: One-and-a-half-year analysis timeframe |
| title_full_unstemmed | Buccal swabs for long-term DNA storage in conservation genetics of fish: One-and-a-half-year analysis timeframe |
| title_short | Buccal swabs for long-term DNA storage in conservation genetics of fish: One-and-a-half-year analysis timeframe |
| title_sort | buccal swabs for long term dna storage in conservation genetics of fish one and a half year analysis timeframe |
| topic | Conservation genetics Principle of the 3Rs DNA long-term storage Aquatic species Biobank |
| url | http://www.sciencedirect.com/science/article/pii/S2351989424000714 |
| work_keys_str_mv | AT simonasciuto buccalswabsforlongtermdnastorageinconservationgeneticsoffishoneandahalfyearanalysistimeframe AT silviacolussi buccalswabsforlongtermdnastorageinconservationgeneticsoffishoneandahalfyearanalysistimeframe AT giuseppeesposito buccalswabsforlongtermdnastorageinconservationgeneticsoffishoneandahalfyearanalysistimeframe AT ariannameletiadis buccalswabsforlongtermdnastorageinconservationgeneticsoffishoneandahalfyearanalysistimeframe AT marinoprearo buccalswabsforlongtermdnastorageinconservationgeneticsoffishoneandahalfyearanalysistimeframe AT elisabettapizzul buccalswabsforlongtermdnastorageinconservationgeneticsoffishoneandahalfyearanalysistimeframe AT pierluigiacutis buccalswabsforlongtermdnastorageinconservationgeneticsoffishoneandahalfyearanalysistimeframe AT rodolpheeliegozlan buccalswabsforlongtermdnastorageinconservationgeneticsoffishoneandahalfyearanalysistimeframe AT paolopastorino buccalswabsforlongtermdnastorageinconservationgeneticsoffishoneandahalfyearanalysistimeframe |