Mitochondrial Factor C20orf7 Facilitates the EMT-Mediated Cancer Cell Migration and the Proliferation of Colon Cancer In Vitro and In Vivo
Colon cancer is a major malignant neoplasm with a low survival rate for late-stage patients. Therefore, the investigation of molecules regulating colon cancer progression and the discovery of novel therapeutic targets is critical. Mitochondria play a vital role in maintaining the homeostasis of cell...
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MDPI AG
2022-11-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/11/2111 |
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| author | Hou-Hsien Liu Chia-Hwa Lee Yi-Chen Hsieh Jia-Huei Zheng Yun-Ru Liu Chia-Hsuan Chang Er-Chieh Cho |
| author_facet | Hou-Hsien Liu Chia-Hwa Lee Yi-Chen Hsieh Jia-Huei Zheng Yun-Ru Liu Chia-Hsuan Chang Er-Chieh Cho |
| author_sort | Hou-Hsien Liu |
| collection | DOAJ |
| description | Colon cancer is a major malignant neoplasm with a low survival rate for late-stage patients. Therefore, the investigation of molecules regulating colon cancer progression and the discovery of novel therapeutic targets is critical. Mitochondria play a vital role in maintaining the homeostasis of cells. Abnormal mitochondrial metabolism alterations and the induction of glycolysis can facilitate tumor growth; therefore, targeting mitochondrial molecules is suggested to be a promising strategy for cancer treatment. In this study, we investigated the role of this largely unknown mitochondrial factor, chromosome 20 open reading frame 7 (C20orf7), in colon cancer progression. Clustered regularly interspaced short palindromic repeats (CRISPR) technology was utilized for C20orf7 depletion, and functional assays were performed to examine the regulation of C20orf7 in colon cancer cells. We demonstrated that C20orf7 facilitates epithelial–mesenchymal transition (EMT)-mediated cell migration and promotes the proliferation of colon cancer. The anti-cancer drug 5-fluorouracil (5FU) was also applied, and C20orf7 was targeted with a combination of 5FU treatment, which could further enhance the anti-cancer effect in the colon cancer cell line and the xenograft mice model. In summary, this study demonstrated, for the first time, that C20orf7 plays a promotional role in cancer tumorigenesis and could be a promising therapeutic target in colon cancer treatment. |
| first_indexed | 2024-03-09T18:19:31Z |
| format | Article |
| id | doaj.art-48aafc9bf7814624a192720c09064efd |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T18:19:31Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-48aafc9bf7814624a192720c09064efd2023-11-24T08:26:28ZengMDPI AGGenes2073-44252022-11-011311211110.3390/genes13112111Mitochondrial Factor C20orf7 Facilitates the EMT-Mediated Cancer Cell Migration and the Proliferation of Colon Cancer In Vitro and In VivoHou-Hsien Liu0Chia-Hwa Lee1Yi-Chen Hsieh2Jia-Huei Zheng3Yun-Ru Liu4Chia-Hsuan Chang5Er-Chieh Cho6School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 110, TaiwanSchool of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 110, TaiwanPh.D. Program in Medical Neuroscience, College of Medical Science and Technology, Taipei Medical University, Taipei 110, TaiwanSchool of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 110, TaiwanJoint Biobank, Office of Human Research, Taipei Medical University, Taipei 110, TaiwanSchool of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 110, TaiwanSchool of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 110, TaiwanColon cancer is a major malignant neoplasm with a low survival rate for late-stage patients. Therefore, the investigation of molecules regulating colon cancer progression and the discovery of novel therapeutic targets is critical. Mitochondria play a vital role in maintaining the homeostasis of cells. Abnormal mitochondrial metabolism alterations and the induction of glycolysis can facilitate tumor growth; therefore, targeting mitochondrial molecules is suggested to be a promising strategy for cancer treatment. In this study, we investigated the role of this largely unknown mitochondrial factor, chromosome 20 open reading frame 7 (C20orf7), in colon cancer progression. Clustered regularly interspaced short palindromic repeats (CRISPR) technology was utilized for C20orf7 depletion, and functional assays were performed to examine the regulation of C20orf7 in colon cancer cells. We demonstrated that C20orf7 facilitates epithelial–mesenchymal transition (EMT)-mediated cell migration and promotes the proliferation of colon cancer. The anti-cancer drug 5-fluorouracil (5FU) was also applied, and C20orf7 was targeted with a combination of 5FU treatment, which could further enhance the anti-cancer effect in the colon cancer cell line and the xenograft mice model. In summary, this study demonstrated, for the first time, that C20orf7 plays a promotional role in cancer tumorigenesis and could be a promising therapeutic target in colon cancer treatment.https://www.mdpi.com/2073-4425/13/11/2111mitochondrial factorC20orf7epithelial–mesenchymal transition (EMT)5-fluorouracil (5FU)colon cancer progressiontherapeutic target |
| spellingShingle | Hou-Hsien Liu Chia-Hwa Lee Yi-Chen Hsieh Jia-Huei Zheng Yun-Ru Liu Chia-Hsuan Chang Er-Chieh Cho Mitochondrial Factor C20orf7 Facilitates the EMT-Mediated Cancer Cell Migration and the Proliferation of Colon Cancer In Vitro and In Vivo Genes mitochondrial factor C20orf7 epithelial–mesenchymal transition (EMT) 5-fluorouracil (5FU) colon cancer progression therapeutic target |
| title | Mitochondrial Factor C20orf7 Facilitates the EMT-Mediated Cancer Cell Migration and the Proliferation of Colon Cancer In Vitro and In Vivo |
| title_full | Mitochondrial Factor C20orf7 Facilitates the EMT-Mediated Cancer Cell Migration and the Proliferation of Colon Cancer In Vitro and In Vivo |
| title_fullStr | Mitochondrial Factor C20orf7 Facilitates the EMT-Mediated Cancer Cell Migration and the Proliferation of Colon Cancer In Vitro and In Vivo |
| title_full_unstemmed | Mitochondrial Factor C20orf7 Facilitates the EMT-Mediated Cancer Cell Migration and the Proliferation of Colon Cancer In Vitro and In Vivo |
| title_short | Mitochondrial Factor C20orf7 Facilitates the EMT-Mediated Cancer Cell Migration and the Proliferation of Colon Cancer In Vitro and In Vivo |
| title_sort | mitochondrial factor c20orf7 facilitates the emt mediated cancer cell migration and the proliferation of colon cancer in vitro and in vivo |
| topic | mitochondrial factor C20orf7 epithelial–mesenchymal transition (EMT) 5-fluorouracil (5FU) colon cancer progression therapeutic target |
| url | https://www.mdpi.com/2073-4425/13/11/2111 |
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