Effects of a Novel, Intelligent, pH-Responsive Resin Adhesive on Cariogenic Biofilms In Vitro
Background: Secondary caries often result in a high failure rate of resin composite restoration. Herein, we studied the dodecylmethylaminoethyl methacrylate–modified resin adhesive (DMAEM@RA) to investigate its pH-responsive antimicrobial effect on <i>Streptococcus mutans</i> and <i&g...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/9/1014 |
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| author | Yangyang Shi Jingou Liang Xuedong Zhou Biao Ren Haohao Wang Qi Han Hao Li Lei Cheng |
| author_facet | Yangyang Shi Jingou Liang Xuedong Zhou Biao Ren Haohao Wang Qi Han Hao Li Lei Cheng |
| author_sort | Yangyang Shi |
| collection | DOAJ |
| description | Background: Secondary caries often result in a high failure rate of resin composite restoration. Herein, we studied the dodecylmethylaminoethyl methacrylate–modified resin adhesive (DMAEM@RA) to investigate its pH-responsive antimicrobial effect on <i>Streptococcus mutans</i> and <i>Candida albicans</i> dual-species biofilms and on secondary caries. Methods: Firstly, the pH-responsive antimicrobial experiments including colony-forming units, scanning electron microscopy and exopoly-saccharide staining were measured. Secondly, lactic acid measurement and transverse microradiography analysis were performed to determine the preventive effect of DMAEM@RA on secondary caries. Lastly, quantitative real-time PCR was applied to investigate the antimicrobial effect of DMAEM@RA on cariogenic virulence genes. Results: DMAEM@RA significantly inhibited the growth, EPS, and acid production of <i>Streptococcus mutans</i> and <i>Candida albicans</i> dual-species biofilms under acidic environments (<i>p</i> < 0.05). Moreover, at pH 5 and 5.5, DMAEM@RA remarkably decreased the mineral loss and lesion depth of tooth hard tissue (<i>p</i> < 0.05) and down-regulated the expression of cariogenic genes, virulence-associated genes, and pH-regulated genes of dual-species biofilms (<i>p</i> < 0.05). Conclusions: DMAEM@RA played an antibiofilm role on <i>Streptococcus mutans</i> and <i>Candida albicans</i> dual-species biofilms, prevented the demineralization process, and attenuated cariogenic virulence in a pH-dependent manner. |
| first_indexed | 2024-03-09T22:53:07Z |
| format | Article |
| id | doaj.art-48aee99f672f4059aa33372eed574fa8 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T22:53:07Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-48aee99f672f4059aa33372eed574fa82023-11-23T18:15:59ZengMDPI AGPathogens2076-08172022-09-01119101410.3390/pathogens11091014Effects of a Novel, Intelligent, pH-Responsive Resin Adhesive on Cariogenic Biofilms In VitroYangyang Shi0Jingou Liang1Xuedong Zhou2Biao Ren3Haohao Wang4Qi Han5Hao Li6Lei Cheng7State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, ChinaBackground: Secondary caries often result in a high failure rate of resin composite restoration. Herein, we studied the dodecylmethylaminoethyl methacrylate–modified resin adhesive (DMAEM@RA) to investigate its pH-responsive antimicrobial effect on <i>Streptococcus mutans</i> and <i>Candida albicans</i> dual-species biofilms and on secondary caries. Methods: Firstly, the pH-responsive antimicrobial experiments including colony-forming units, scanning electron microscopy and exopoly-saccharide staining were measured. Secondly, lactic acid measurement and transverse microradiography analysis were performed to determine the preventive effect of DMAEM@RA on secondary caries. Lastly, quantitative real-time PCR was applied to investigate the antimicrobial effect of DMAEM@RA on cariogenic virulence genes. Results: DMAEM@RA significantly inhibited the growth, EPS, and acid production of <i>Streptococcus mutans</i> and <i>Candida albicans</i> dual-species biofilms under acidic environments (<i>p</i> < 0.05). Moreover, at pH 5 and 5.5, DMAEM@RA remarkably decreased the mineral loss and lesion depth of tooth hard tissue (<i>p</i> < 0.05) and down-regulated the expression of cariogenic genes, virulence-associated genes, and pH-regulated genes of dual-species biofilms (<i>p</i> < 0.05). Conclusions: DMAEM@RA played an antibiofilm role on <i>Streptococcus mutans</i> and <i>Candida albicans</i> dual-species biofilms, prevented the demineralization process, and attenuated cariogenic virulence in a pH-dependent manner.https://www.mdpi.com/2076-0817/11/9/1014<i>Streptococcus mutans</i><i>Candida albicans</i>secondary cariesantimicrobial effectpH response |
| spellingShingle | Yangyang Shi Jingou Liang Xuedong Zhou Biao Ren Haohao Wang Qi Han Hao Li Lei Cheng Effects of a Novel, Intelligent, pH-Responsive Resin Adhesive on Cariogenic Biofilms In Vitro Pathogens <i>Streptococcus mutans</i> <i>Candida albicans</i> secondary caries antimicrobial effect pH response |
| title | Effects of a Novel, Intelligent, pH-Responsive Resin Adhesive on Cariogenic Biofilms In Vitro |
| title_full | Effects of a Novel, Intelligent, pH-Responsive Resin Adhesive on Cariogenic Biofilms In Vitro |
| title_fullStr | Effects of a Novel, Intelligent, pH-Responsive Resin Adhesive on Cariogenic Biofilms In Vitro |
| title_full_unstemmed | Effects of a Novel, Intelligent, pH-Responsive Resin Adhesive on Cariogenic Biofilms In Vitro |
| title_short | Effects of a Novel, Intelligent, pH-Responsive Resin Adhesive on Cariogenic Biofilms In Vitro |
| title_sort | effects of a novel intelligent ph responsive resin adhesive on cariogenic biofilms in vitro |
| topic | <i>Streptococcus mutans</i> <i>Candida albicans</i> secondary caries antimicrobial effect pH response |
| url | https://www.mdpi.com/2076-0817/11/9/1014 |
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