Characterization of Exogenous Sequence Fragments in Extracellular Vesicles from Human
Extracellular vesicles (EVs) play crucial role in mediating intercellular communication. Small RNA is an important component in EVs. However, the proportion of small RNA sequencing (smRNA‐seq) reads in EVs mapped to the human genome is much lower than that of cells, suggesting the existence of many...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2023-09-01
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| Series: | Small Structures |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/sstr.202200406 |
| _version_ | 1797685004923305984 |
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| author | Yi Wang Gui-Yan Xie Qiong Zhang Xiuqing Zhang An-Yuan Guo |
| author_facet | Yi Wang Gui-Yan Xie Qiong Zhang Xiuqing Zhang An-Yuan Guo |
| author_sort | Yi Wang |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) play crucial role in mediating intercellular communication. Small RNA is an important component in EVs. However, the proportion of small RNA sequencing (smRNA‐seq) reads in EVs mapped to the human genome is much lower than that of cells, suggesting the existence of many nonhuman sequences in EVs. However, there is no systematic study on EV fragments unmapped to the human genome. Herein, using EV smRNA‐seq data, the landscape of exogenous RNA cargoes in human EVs is portrayed. The results show the distribution of nonhuman sequence fragments in 1838 EV samples; an average of 21.82% of reads are unmapped to the human genome, and 12.33% are mapped to the collected exogenous reference sequences. Furthermore, the proportion of exogenous sequences in plasma EV samples is the lowest, while in the cell line EV samples, it is much higher, mainly from animals, bacteria, or contaminants. Exogenous sequences from plants are mainly from food, and the exogenous bacteria are mainly gut microbiota. Virus‐derived sequences reflect the high prevalence of viruses in the population, such as herpesvirus and hepatitis virus. This study provides the first landscape of exogenous fragments in human EVs and implies diverse RNA sources in the human body. |
| first_indexed | 2024-03-12T00:39:00Z |
| format | Article |
| id | doaj.art-48af179220444e37a63ee0c9814f3c32 |
| institution | Directory Open Access Journal |
| issn | 2688-4062 |
| language | English |
| last_indexed | 2024-03-12T00:39:00Z |
| publishDate | 2023-09-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Small Structures |
| spelling | doaj.art-48af179220444e37a63ee0c9814f3c322023-09-15T09:17:18ZengWiley-VCHSmall Structures2688-40622023-09-0149n/an/a10.1002/sstr.202200406Characterization of Exogenous Sequence Fragments in Extracellular Vesicles from HumanYi Wang0Gui-Yan Xie1Qiong Zhang2Xiuqing Zhang3An-Yuan Guo4College of Life Sciences University of Chinese Academy of Sciences Beijing 100049 ChinaWest China Biomedical Big Data Center West China Hospital/West China School of Medicine Med-X Center for Informatics Sichuan University Chengdu 610041 ChinaResearch Center of Clinical Medicine Affiliated Hospital of Nantong University Nantong 226001 ChinaCollege of Life Sciences University of Chinese Academy of Sciences Beijing 100049 ChinaWest China Biomedical Big Data Center West China Hospital/West China School of Medicine Med-X Center for Informatics Sichuan University Chengdu 610041 ChinaExtracellular vesicles (EVs) play crucial role in mediating intercellular communication. Small RNA is an important component in EVs. However, the proportion of small RNA sequencing (smRNA‐seq) reads in EVs mapped to the human genome is much lower than that of cells, suggesting the existence of many nonhuman sequences in EVs. However, there is no systematic study on EV fragments unmapped to the human genome. Herein, using EV smRNA‐seq data, the landscape of exogenous RNA cargoes in human EVs is portrayed. The results show the distribution of nonhuman sequence fragments in 1838 EV samples; an average of 21.82% of reads are unmapped to the human genome, and 12.33% are mapped to the collected exogenous reference sequences. Furthermore, the proportion of exogenous sequences in plasma EV samples is the lowest, while in the cell line EV samples, it is much higher, mainly from animals, bacteria, or contaminants. Exogenous sequences from plants are mainly from food, and the exogenous bacteria are mainly gut microbiota. Virus‐derived sequences reflect the high prevalence of viruses in the population, such as herpesvirus and hepatitis virus. This study provides the first landscape of exogenous fragments in human EVs and implies diverse RNA sources in the human body.https://doi.org/10.1002/sstr.202200406exogenous fragmentsextracellular vesiclesfoodnonhuman sequencessmall RNA |
| spellingShingle | Yi Wang Gui-Yan Xie Qiong Zhang Xiuqing Zhang An-Yuan Guo Characterization of Exogenous Sequence Fragments in Extracellular Vesicles from Human Small Structures exogenous fragments extracellular vesicles food nonhuman sequences small RNA |
| title | Characterization of Exogenous Sequence Fragments in Extracellular Vesicles from Human |
| title_full | Characterization of Exogenous Sequence Fragments in Extracellular Vesicles from Human |
| title_fullStr | Characterization of Exogenous Sequence Fragments in Extracellular Vesicles from Human |
| title_full_unstemmed | Characterization of Exogenous Sequence Fragments in Extracellular Vesicles from Human |
| title_short | Characterization of Exogenous Sequence Fragments in Extracellular Vesicles from Human |
| title_sort | characterization of exogenous sequence fragments in extracellular vesicles from human |
| topic | exogenous fragments extracellular vesicles food nonhuman sequences small RNA |
| url | https://doi.org/10.1002/sstr.202200406 |
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