Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crosses

This study presents the first report comparing the efficiency of microspore culture, gynogenesis and durum wheat × maize crosses for haploid plant production from three durum wheat genotypes (Razzek, Karim and Jneh Khotifa). The results showed that the best induction, calli or embryos formation and...

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Main Authors: Olfa Slama-Ayed, Imen Bouhaouel, Sourour Ayed, Jacques De Buyser, Emmanuel Picard, Hajer Slim Amara
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2019-09-01
Series:Czech Journal of Genetics and Plant Breeding
Subjects:
Online Access:https://cjgpb.agriculturejournals.cz/artkey/cjg-201903-0003_efficiency-of-three-haplomethods-in-durum-wheat-triticum-turgidum-subsp-durum-desf-isolated-microspore-cul.php
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author Olfa Slama-Ayed
Imen Bouhaouel
Sourour Ayed
Jacques De Buyser
Emmanuel Picard
Hajer Slim Amara
author_facet Olfa Slama-Ayed
Imen Bouhaouel
Sourour Ayed
Jacques De Buyser
Emmanuel Picard
Hajer Slim Amara
author_sort Olfa Slama-Ayed
collection DOAJ
description This study presents the first report comparing the efficiency of microspore culture, gynogenesis and durum wheat × maize crosses for haploid plant production from three durum wheat genotypes (Razzek, Karim and Jneh Khotifa). The results showed that the best induction, calli or embryos formation and plant regeneration rates for the three genotypes were obtained with gynogenesis (47.2, 7.6, 0.8%), followed by interspecific crosses (33.1, 1.7, 0.4%) and isolated microspore culture (8.2, 0.05, 0.01%). Interestingly, all plants regenerated by gynogenesis and durum wheat × maize crosses were green whereas all plants obtained by isolated microspore culture were albino. In the haploid production system, all steps of the process are important for the three methods. The critical steps that have greatly reduced the number of regenerated haploid plants were induction, embryogenesis and regeneration for microspore culture, forming and regeneration of calli or embryo and haploid regeneration for interspecific crosses and gynogenesis. Genotypes with good capacity of induction have not necessarily a good capacity of haploid plantlets regeneration and vice-versa. However, calli or embryos formation seems to be an indicator of the haploid production. Overall, Razzek showed a good ability to produce haploids using the three methods. Each haplomethod showed a specific advantage. Although gynogenesis is the less used method for durum wheat, it has proved to be a successful approach for green haploid plant production.
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spelling doaj.art-48b357911c8a4a1fab9c99167d5e356c2023-02-23T03:30:46ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252019-09-0155310110910.17221/188/2017-CJGPBcjg-201903-0003Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crossesOlfa Slama-Ayed0Imen Bouhaouel1Sourour Ayed2Jacques De Buyser3Emmanuel Picard4Hajer Slim Amara5Genetic and Cereal Breeding Laboratory, National Agronomic Institute of Tunisia, University of Carthage, Tunis Mahrajene, TunisiaGenetic and Cereal Breeding Laboratory, National Agronomic Institute of Tunisia, University of Carthage, Tunis Mahrajene, TunisiaGenetic and Cereal Breeding Laboratory, National Agronomic Institute of Tunisia, University of Carthage, Tunis Mahrajene, TunisiaMVEH Laboratory, Paris-Sud University, Orsay, FranceMVEH Laboratory, Paris-Sud University, Orsay, FranceGenetic and Cereal Breeding Laboratory, National Agronomic Institute of Tunisia, University of Carthage, Tunis Mahrajene, TunisiaThis study presents the first report comparing the efficiency of microspore culture, gynogenesis and durum wheat × maize crosses for haploid plant production from three durum wheat genotypes (Razzek, Karim and Jneh Khotifa). The results showed that the best induction, calli or embryos formation and plant regeneration rates for the three genotypes were obtained with gynogenesis (47.2, 7.6, 0.8%), followed by interspecific crosses (33.1, 1.7, 0.4%) and isolated microspore culture (8.2, 0.05, 0.01%). Interestingly, all plants regenerated by gynogenesis and durum wheat × maize crosses were green whereas all plants obtained by isolated microspore culture were albino. In the haploid production system, all steps of the process are important for the three methods. The critical steps that have greatly reduced the number of regenerated haploid plants were induction, embryogenesis and regeneration for microspore culture, forming and regeneration of calli or embryo and haploid regeneration for interspecific crosses and gynogenesis. Genotypes with good capacity of induction have not necessarily a good capacity of haploid plantlets regeneration and vice-versa. However, calli or embryos formation seems to be an indicator of the haploid production. Overall, Razzek showed a good ability to produce haploids using the three methods. Each haplomethod showed a specific advantage. Although gynogenesis is the less used method for durum wheat, it has proved to be a successful approach for green haploid plant production.https://cjgpb.agriculturejournals.cz/artkey/cjg-201903-0003_efficiency-of-three-haplomethods-in-durum-wheat-triticum-turgidum-subsp-durum-desf-isolated-microspore-cul.phpandrogenesishaploidizationinterspecific crossesunpollinated ovary culturewheat
spellingShingle Olfa Slama-Ayed
Imen Bouhaouel
Sourour Ayed
Jacques De Buyser
Emmanuel Picard
Hajer Slim Amara
Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crosses
Czech Journal of Genetics and Plant Breeding
androgenesis
haploidization
interspecific crosses
unpollinated ovary culture
wheat
title Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crosses
title_full Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crosses
title_fullStr Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crosses
title_full_unstemmed Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crosses
title_short Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crosses
title_sort efficiency of three haplomethods in durum wheat triticum turgidum subsp durum desf isolated microspore culture gynogenesis and wheat maize crosses
topic androgenesis
haploidization
interspecific crosses
unpollinated ovary culture
wheat
url https://cjgpb.agriculturejournals.cz/artkey/cjg-201903-0003_efficiency-of-three-haplomethods-in-durum-wheat-triticum-turgidum-subsp-durum-desf-isolated-microspore-cul.php
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