Liposomal Formulations to Improve Antioxidant Power of Myrtle Berry Extract for Potential Skin Application
Many substances in plant extracts are known for their biological activities. These substances act in different ways, exerting overall protective effects against many diseases, especially skin disorders. However, plant extracts’ health benefits are often limited by low bioavailability. To overcome th...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/14/5/910 |
| _version_ | 1797496773336367104 |
|---|---|
| author | Maria De Luca Daniela Lucchesi Carlo Ignazio Giovanni Tuberoso Xavier Fernàndez-Busquets Antonio Vassallo Giuseppe Martelli Anna Maria Fadda Laura Pucci Carla Caddeo |
| author_facet | Maria De Luca Daniela Lucchesi Carlo Ignazio Giovanni Tuberoso Xavier Fernàndez-Busquets Antonio Vassallo Giuseppe Martelli Anna Maria Fadda Laura Pucci Carla Caddeo |
| author_sort | Maria De Luca |
| collection | DOAJ |
| description | Many substances in plant extracts are known for their biological activities. These substances act in different ways, exerting overall protective effects against many diseases, especially skin disorders. However, plant extracts’ health benefits are often limited by low bioavailability. To overcome these limitations, drug delivery systems can be employed. In this study, we evaluated the antioxidant power of an ethanolic extract from <i>Myrtus communis</i> L. (myrtle) berries through colorimetric tests (DPPH and FRAP). The antioxidant activity was also verified by using fibroblast cell culture through cellular Reactive Oxygen Species (ROS) levels measurements. Moreover, the myrtle extract was formulated in phospholipid vesicles to improve its bioavailability and applicability. Myrtle liposomes were characterized by size, surface charge, storage stability, and entrapment efficiency; visualized by using cryo-TEM images; and assayed for cytocompatibility and anti-ROS activity. Our results suggest that myrtle liposomes were cytocompatible and improved the extract’s antioxidant power in fibroblasts, suggesting a potential skin application for these formulations and confirming that nanotechnologies could be a valid tool to enhance plant extracts’ potentialities. |
| first_indexed | 2024-03-10T03:08:25Z |
| format | Article |
| id | doaj.art-48b381d577684120b8a1aa5f6c74e6d6 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T03:08:25Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-48b381d577684120b8a1aa5f6c74e6d62023-11-23T12:36:19ZengMDPI AGPharmaceutics1999-49232022-04-0114591010.3390/pharmaceutics14050910Liposomal Formulations to Improve Antioxidant Power of Myrtle Berry Extract for Potential Skin ApplicationMaria De Luca0Daniela Lucchesi1Carlo Ignazio Giovanni Tuberoso2Xavier Fernàndez-Busquets3Antonio Vassallo4Giuseppe Martelli5Anna Maria Fadda6Laura Pucci7Carla Caddeo8Department of Scienze, University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, ItalySection of Diabetes and Metabolic Diseases, Department of Clinical and Experimental Medicine, University of Pisa, Via Piero Trivella, 56124 Pisa, ItalyDepartment of Scienze della Vita e dell’Ambiente, University of Cagliari, S.P. Monserrato-Sestu Km 0.700, 09042 Monserrato, ItalyNanomalaria Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Baldiri Reixac 10-12, 08028 Barcelona, SpainDepartment of Scienze, University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, ItalyDepartment of Scienze, University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, ItalyDepartment of Scienze della Vita e dell’Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, 09124 Cagliari, ItalyInstitute of Agricultural Biology and Biotechnology, CNR Pisa, Via Moruzzi 1, 56124 Pisa, ItalyDepartment of Scienze della Vita e dell’Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, 09124 Cagliari, ItalyMany substances in plant extracts are known for their biological activities. These substances act in different ways, exerting overall protective effects against many diseases, especially skin disorders. However, plant extracts’ health benefits are often limited by low bioavailability. To overcome these limitations, drug delivery systems can be employed. In this study, we evaluated the antioxidant power of an ethanolic extract from <i>Myrtus communis</i> L. (myrtle) berries through colorimetric tests (DPPH and FRAP). The antioxidant activity was also verified by using fibroblast cell culture through cellular Reactive Oxygen Species (ROS) levels measurements. Moreover, the myrtle extract was formulated in phospholipid vesicles to improve its bioavailability and applicability. Myrtle liposomes were characterized by size, surface charge, storage stability, and entrapment efficiency; visualized by using cryo-TEM images; and assayed for cytocompatibility and anti-ROS activity. Our results suggest that myrtle liposomes were cytocompatible and improved the extract’s antioxidant power in fibroblasts, suggesting a potential skin application for these formulations and confirming that nanotechnologies could be a valid tool to enhance plant extracts’ potentialities.https://www.mdpi.com/1999-4923/14/5/910myrtle extractliposomesantioxidantfibroblastskin |
| spellingShingle | Maria De Luca Daniela Lucchesi Carlo Ignazio Giovanni Tuberoso Xavier Fernàndez-Busquets Antonio Vassallo Giuseppe Martelli Anna Maria Fadda Laura Pucci Carla Caddeo Liposomal Formulations to Improve Antioxidant Power of Myrtle Berry Extract for Potential Skin Application Pharmaceutics myrtle extract liposomes antioxidant fibroblast skin |
| title | Liposomal Formulations to Improve Antioxidant Power of Myrtle Berry Extract for Potential Skin Application |
| title_full | Liposomal Formulations to Improve Antioxidant Power of Myrtle Berry Extract for Potential Skin Application |
| title_fullStr | Liposomal Formulations to Improve Antioxidant Power of Myrtle Berry Extract for Potential Skin Application |
| title_full_unstemmed | Liposomal Formulations to Improve Antioxidant Power of Myrtle Berry Extract for Potential Skin Application |
| title_short | Liposomal Formulations to Improve Antioxidant Power of Myrtle Berry Extract for Potential Skin Application |
| title_sort | liposomal formulations to improve antioxidant power of myrtle berry extract for potential skin application |
| topic | myrtle extract liposomes antioxidant fibroblast skin |
| url | https://www.mdpi.com/1999-4923/14/5/910 |
| work_keys_str_mv | AT mariadeluca liposomalformulationstoimproveantioxidantpowerofmyrtleberryextractforpotentialskinapplication AT danielalucchesi liposomalformulationstoimproveantioxidantpowerofmyrtleberryextractforpotentialskinapplication AT carloignaziogiovannituberoso liposomalformulationstoimproveantioxidantpowerofmyrtleberryextractforpotentialskinapplication AT xavierfernandezbusquets liposomalformulationstoimproveantioxidantpowerofmyrtleberryextractforpotentialskinapplication AT antoniovassallo liposomalformulationstoimproveantioxidantpowerofmyrtleberryextractforpotentialskinapplication AT giuseppemartelli liposomalformulationstoimproveantioxidantpowerofmyrtleberryextractforpotentialskinapplication AT annamariafadda liposomalformulationstoimproveantioxidantpowerofmyrtleberryextractforpotentialskinapplication AT laurapucci liposomalformulationstoimproveantioxidantpowerofmyrtleberryextractforpotentialskinapplication AT carlacaddeo liposomalformulationstoimproveantioxidantpowerofmyrtleberryextractforpotentialskinapplication |