An integrated analysis of lncRNA and mRNA expression profiles in the kidneys of mice with lupus nephritis
Long noncoding RNAs (lncRNAs) are persistently expressed and have been described as potential biomarkers and therapeutic targets in various diseases. However, there is limited information regarding lncRNA expression in the tissue of kidney exhibiting lupus nephritis (LN)a serious complication of sys...
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PeerJ Inc.
2021-02-01
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| author | Juan Wang Xiongfei Wu Yafang Tu Jianzhong Dang Zhitao Cai Wenjing Liao Weili Quan Yaxun Wei |
| author_facet | Juan Wang Xiongfei Wu Yafang Tu Jianzhong Dang Zhitao Cai Wenjing Liao Weili Quan Yaxun Wei |
| author_sort | Juan Wang |
| collection | DOAJ |
| description | Long noncoding RNAs (lncRNAs) are persistently expressed and have been described as potential biomarkers and therapeutic targets in various diseases. However, there is limited information regarding lncRNA expression in the tissue of kidney exhibiting lupus nephritis (LN)a serious complication of systemic lupus erythematosus (SLE). In this study, RNA sequencing (RNA-seq) was performed to characterize the lncRNA and mRNA expression in kidney tissues from LN (MRL/lpr) and control mice. We identified 12,979 novel lncRNAs in mouse. The expression profiles of both mRNAs and lncRNAs were differed significantly between LN and control mice. In particular, there were more upregulated lncRNAs and mRNAs than downregulated ones in the kidney tissues of LN mice. However, GO analysis showed that more downregulated genes were enriched in immune and inflammatory response-associated pathways. KEGG analysis showed that both downregulated and upregulated genes were enriched in a number of pathways, including the SLE pathway, and approximately half of these SLE-associated genes encoded inflammatory factors. Moreover, we observed that 2,181 DElncRNAs may have targeted and regulated the expression of 778 mRNAs in LN kidney tissues. The results of this study showed that 11 DElncRNAs targeted and were co-expressed with six immune and SLE-associated genes. qPCR analysis confirmed that lncRNA Gm20513 positively regulated the expression of the SLE-associated gene H2-Aa. In conclusion, the results of our study demonstrates that lncRNAs influence the progression of LN and provide some cues for further study of lncRNAs in LN. These results regarding the lncRNA-mRNAregulatory network may have important value in LN diagnosis and therapy. |
| first_indexed | 2024-03-09T08:07:25Z |
| format | Article |
| id | doaj.art-48b3891cdb33440e870ccb39b4505762 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:07:25Z |
| publishDate | 2021-02-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-48b3891cdb33440e870ccb39b45057622023-12-02T23:45:18ZengPeerJ Inc.PeerJ2167-83592021-02-019e1066810.7717/peerj.10668An integrated analysis of lncRNA and mRNA expression profiles in the kidneys of mice with lupus nephritisJuan Wang0Xiongfei Wu1Yafang Tu2Jianzhong Dang3Zhitao Cai4Wenjing Liao5Weili Quan6Yaxun Wei7Nephrology Department, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaNephrology Department, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaNephrology Department, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaNephrology Department, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaNephrology Department, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaNephrology Department, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaABLife BioBigData Institute, Wuhan, Hubei, ChinaCenter for Genome Analysis, ABLife Inc., Wuhan, Hubei, ChinaLong noncoding RNAs (lncRNAs) are persistently expressed and have been described as potential biomarkers and therapeutic targets in various diseases. However, there is limited information regarding lncRNA expression in the tissue of kidney exhibiting lupus nephritis (LN)a serious complication of systemic lupus erythematosus (SLE). In this study, RNA sequencing (RNA-seq) was performed to characterize the lncRNA and mRNA expression in kidney tissues from LN (MRL/lpr) and control mice. We identified 12,979 novel lncRNAs in mouse. The expression profiles of both mRNAs and lncRNAs were differed significantly between LN and control mice. In particular, there were more upregulated lncRNAs and mRNAs than downregulated ones in the kidney tissues of LN mice. However, GO analysis showed that more downregulated genes were enriched in immune and inflammatory response-associated pathways. KEGG analysis showed that both downregulated and upregulated genes were enriched in a number of pathways, including the SLE pathway, and approximately half of these SLE-associated genes encoded inflammatory factors. Moreover, we observed that 2,181 DElncRNAs may have targeted and regulated the expression of 778 mRNAs in LN kidney tissues. The results of this study showed that 11 DElncRNAs targeted and were co-expressed with six immune and SLE-associated genes. qPCR analysis confirmed that lncRNA Gm20513 positively regulated the expression of the SLE-associated gene H2-Aa. In conclusion, the results of our study demonstrates that lncRNAs influence the progression of LN and provide some cues for further study of lncRNAs in LN. These results regarding the lncRNA-mRNAregulatory network may have important value in LN diagnosis and therapy.https://peerj.com/articles/10668.pdfLupus nephritisRNA sequencinglncRNAsDifferentially expressed genesImmune response |
| spellingShingle | Juan Wang Xiongfei Wu Yafang Tu Jianzhong Dang Zhitao Cai Wenjing Liao Weili Quan Yaxun Wei An integrated analysis of lncRNA and mRNA expression profiles in the kidneys of mice with lupus nephritis PeerJ Lupus nephritis RNA sequencing lncRNAs Differentially expressed genes Immune response |
| title | An integrated analysis of lncRNA and mRNA expression profiles in the kidneys of mice with lupus nephritis |
| title_full | An integrated analysis of lncRNA and mRNA expression profiles in the kidneys of mice with lupus nephritis |
| title_fullStr | An integrated analysis of lncRNA and mRNA expression profiles in the kidneys of mice with lupus nephritis |
| title_full_unstemmed | An integrated analysis of lncRNA and mRNA expression profiles in the kidneys of mice with lupus nephritis |
| title_short | An integrated analysis of lncRNA and mRNA expression profiles in the kidneys of mice with lupus nephritis |
| title_sort | integrated analysis of lncrna and mrna expression profiles in the kidneys of mice with lupus nephritis |
| topic | Lupus nephritis RNA sequencing lncRNAs Differentially expressed genes Immune response |
| url | https://peerj.com/articles/10668.pdf |
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