Enhancement of Immune Activities of Mixtures with <i>Sasa quelpaertensis</i> Nakai and <i>Ficus erecta</i> var. <i>sieboldii</i>
The objective of the present study was to develop a concoction of natural products that could dramatically improve immune function with minimal possible side effects. <i>Sasa quelpaertensis</i> Nakai and <i>Ficus erecta</i> var. <i>sieboldii</i> are plants that ar...
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MDPI AG
2020-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/7/868 |
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| author | Hee-Yeon Kwon Sun-Il Choi Xionggao Han Xiao Men Gill-Woong Jang Ye-Eun Choi Seung-Hyung Kim Jun-Chul Kang Ju-Hyun Cho Ok-Hwan Lee |
| author_facet | Hee-Yeon Kwon Sun-Il Choi Xionggao Han Xiao Men Gill-Woong Jang Ye-Eun Choi Seung-Hyung Kim Jun-Chul Kang Ju-Hyun Cho Ok-Hwan Lee |
| author_sort | Hee-Yeon Kwon |
| collection | DOAJ |
| description | The objective of the present study was to develop a concoction of natural products that could dramatically improve immune function with minimal possible side effects. <i>Sasa quelpaertensis</i> Nakai and <i>Ficus erecta</i> var. <i>sieboldii</i> are plants that are native to Jeju Island, Korea and are known to be rich in physiologically active substances. We prepared a mixture of different proportions and extraction conditions using two natural plants and determined their optimum mixing ratio and extraction method by assessing immune function-related biomarkers in RAW264.7 macrophages. Optimal extract (HR02/04(8:2)-W) was selected from in vitro experiments and its immunity-enhancing efficacy was evaluated in mice. After oral administration of extract to BALB/c mice for 2 weeks, nitric oxide production in the peritoneal exudate cells, natural killer cell cytotoxicity, cytokine expression in splenocytes, and total cell number of immune tissues and phenotype analysis were evaluated. Our results demonstrated that HR02/04(8:2)-W significantly enhanced the immune system by increasing natural killer cell activity, cytokine expression, and total number of cells in immune tissues. In conclusion, our study validates the role of HR02/04(8:2)-W in enhancing immunity and its potential development as a functional food. |
| first_indexed | 2024-03-10T18:42:24Z |
| format | Article |
| id | doaj.art-48b6f616170a464584f97d935eaa2a66 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T18:42:24Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-48b6f616170a464584f97d935eaa2a662023-11-20T05:42:23ZengMDPI AGFoods2304-81582020-07-019786810.3390/foods9070868Enhancement of Immune Activities of Mixtures with <i>Sasa quelpaertensis</i> Nakai and <i>Ficus erecta</i> var. <i>sieboldii</i>Hee-Yeon Kwon0Sun-Il Choi1Xionggao Han2Xiao Men3Gill-Woong Jang4Ye-Eun Choi5Seung-Hyung Kim6Jun-Chul Kang7Ju-Hyun Cho8Ok-Hwan Lee9Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, KoreaDepartment of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, KoreaDepartment of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, KoreaDepartment of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, KoreaDepartment of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, KoreaDepartment of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, KoreaInstitute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 34520, KoreaHaram Central Research Institute, Cheongju 28160, KoreaHaram Central Research Institute, Cheongju 28160, KoreaDepartment of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, KoreaThe objective of the present study was to develop a concoction of natural products that could dramatically improve immune function with minimal possible side effects. <i>Sasa quelpaertensis</i> Nakai and <i>Ficus erecta</i> var. <i>sieboldii</i> are plants that are native to Jeju Island, Korea and are known to be rich in physiologically active substances. We prepared a mixture of different proportions and extraction conditions using two natural plants and determined their optimum mixing ratio and extraction method by assessing immune function-related biomarkers in RAW264.7 macrophages. Optimal extract (HR02/04(8:2)-W) was selected from in vitro experiments and its immunity-enhancing efficacy was evaluated in mice. After oral administration of extract to BALB/c mice for 2 weeks, nitric oxide production in the peritoneal exudate cells, natural killer cell cytotoxicity, cytokine expression in splenocytes, and total cell number of immune tissues and phenotype analysis were evaluated. Our results demonstrated that HR02/04(8:2)-W significantly enhanced the immune system by increasing natural killer cell activity, cytokine expression, and total number of cells in immune tissues. In conclusion, our study validates the role of HR02/04(8:2)-W in enhancing immunity and its potential development as a functional food.https://www.mdpi.com/2304-8158/9/7/868immune activities<i>Sasa quelpaertensis</i> Nakai<i>Ficus erecta</i> var. <i>sieboldii</i>RAW264.7mixture |
| spellingShingle | Hee-Yeon Kwon Sun-Il Choi Xionggao Han Xiao Men Gill-Woong Jang Ye-Eun Choi Seung-Hyung Kim Jun-Chul Kang Ju-Hyun Cho Ok-Hwan Lee Enhancement of Immune Activities of Mixtures with <i>Sasa quelpaertensis</i> Nakai and <i>Ficus erecta</i> var. <i>sieboldii</i> Foods immune activities <i>Sasa quelpaertensis</i> Nakai <i>Ficus erecta</i> var. <i>sieboldii</i> RAW264.7 mixture |
| title | Enhancement of Immune Activities of Mixtures with <i>Sasa quelpaertensis</i> Nakai and <i>Ficus erecta</i> var. <i>sieboldii</i> |
| title_full | Enhancement of Immune Activities of Mixtures with <i>Sasa quelpaertensis</i> Nakai and <i>Ficus erecta</i> var. <i>sieboldii</i> |
| title_fullStr | Enhancement of Immune Activities of Mixtures with <i>Sasa quelpaertensis</i> Nakai and <i>Ficus erecta</i> var. <i>sieboldii</i> |
| title_full_unstemmed | Enhancement of Immune Activities of Mixtures with <i>Sasa quelpaertensis</i> Nakai and <i>Ficus erecta</i> var. <i>sieboldii</i> |
| title_short | Enhancement of Immune Activities of Mixtures with <i>Sasa quelpaertensis</i> Nakai and <i>Ficus erecta</i> var. <i>sieboldii</i> |
| title_sort | enhancement of immune activities of mixtures with i sasa quelpaertensis i nakai and i ficus erecta i var i sieboldii i |
| topic | immune activities <i>Sasa quelpaertensis</i> Nakai <i>Ficus erecta</i> var. <i>sieboldii</i> RAW264.7 mixture |
| url | https://www.mdpi.com/2304-8158/9/7/868 |
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