Tunisian Native <i>Mentha pulegium</i> L. Extracts: Phytochemical Composition and Biological Activities
Mint species (<i>Lamiaceae</i> family) have been used as traditional remedies for the treatment of several diseases. In this work, we aimed to characterize the biological activities of the total phenolic and flavonoid contents of <i>Mentha pulegium</i> L. extracts collected f...
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/1/314 |
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| author | Jed Jebali Hanene Ghazghazi Chedia Aouadhi Ines ELBini-Dhouib Ridha Ben Salem Najet Srairi-Abid Naziha Marrakchi Ghayth Rigane |
| author_facet | Jed Jebali Hanene Ghazghazi Chedia Aouadhi Ines ELBini-Dhouib Ridha Ben Salem Najet Srairi-Abid Naziha Marrakchi Ghayth Rigane |
| author_sort | Jed Jebali |
| collection | DOAJ |
| description | Mint species (<i>Lamiaceae</i> family) have been used as traditional remedies for the treatment of several diseases. In this work, we aimed to characterize the biological activities of the total phenolic and flavonoid contents of <i>Mentha pulegium</i> L. extracts collected from two different regions of Tunisia. The highest amounts of total phenols (74.45 ± 0.01 mg GAE/g DW), flavonoids (28.87 ± 0.02 mg RE/g DW), and condensed tannins (4.35 ± 0.02 mg CE/g DW) were found in the Bizerte locality. Methanolic leaf extracts were subjected to HPLC-UV analysis in order to identify and quantify the phenolic composition. This technique allowed us to identify seven phenolic compounds: two phenolic acids and five flavonoid compounds, such as eriocitrin, hesperidin, narirutin, luteolin, and isorhoifolin, which were found in both extracts with significant differences between samples collected from the different regions (<i>p</i> < 0.05). Furthermore, our results showed that the methanolic extract from leaves collected from Bizerte had the highest antioxidant activities (DPPH IC<sub>50</sub> value of 16.31 μg/mL and 570.08 μmol Fe<sup>2+</sup>/g, respectively). Both extracts showed high radical-scavenging activity as well as significant antimicrobial activity against eight tested bacteria. The highest antimicrobial activities were observed against Gram-positive bacteria with inhibition zone diameters and MIC values ranging between 19 and 32 mm and 40 and 160 µg/mL, respectively. Interestingly, at 10 μg/mL, the extract had a significant effect on cell proliferation of U87 human glioblastoma cells. These findings open perspectives for the use of <i>Mentha pulegium</i> L. extract in green pharmacy, alternative/complementary medicine, and natural preventive therapies for the development of effective antioxidant, antibacterial, and/or antitumoral drugs. |
| first_indexed | 2024-03-10T03:29:46Z |
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| id | doaj.art-48b8c437c6674327ba7e6691ffe48959 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:29:46Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-48b8c437c6674327ba7e6691ffe489592023-11-23T11:59:49ZengMDPI AGMolecules1420-30492022-01-0127131410.3390/molecules27010314Tunisian Native <i>Mentha pulegium</i> L. Extracts: Phytochemical Composition and Biological ActivitiesJed Jebali0Hanene Ghazghazi1Chedia Aouadhi2Ines ELBini-Dhouib3Ridha Ben Salem4Najet Srairi-Abid5Naziha Marrakchi6Ghayth Rigane7Laboratory of Biomolecules, Venoms and Theranostic Applications, LR20IPT01, Institut Pasteur of Tunis, University of Tunis El Manar, Tunis 1002, TunisiaLaboratory of Management and Valorization of Forest Resources, National Research Institute of Rural Engineering, Water and Forestry (INRGREF), University of Carthage, Tunis 1004, TunisiaLaboratoire d’Epidémiologie et Microbiologie Vétérinaire, Groupes de Bactériologie et Développement Biotechnologique, Institut Pasteur de Tunis, Université de Tunis El Manar, 13, Place Pasteur, B.P. 74, Tunis 1002, TunisiaLaboratory of Biomolecules, Venoms and Theranostic Applications, LR20IPT01, Institut Pasteur of Tunis, University of Tunis El Manar, Tunis 1002, TunisiaLaboratory of Organic Chemistry LR17ES08, Faculty of Sciences of Sfax, University of Sfax, B.P. 1171, Sfax 3038, TunisiaLaboratory of Biomolecules, Venoms and Theranostic Applications, LR20IPT01, Institut Pasteur of Tunis, University of Tunis El Manar, Tunis 1002, TunisiaLaboratory of Biomolecules, Venoms and Theranostic Applications, LR20IPT01, Institut Pasteur of Tunis, University of Tunis El Manar, Tunis 1002, TunisiaLaboratory of Organic Chemistry LR17ES08, Faculty of Sciences of Sfax, University of Sfax, B.P. 1171, Sfax 3038, TunisiaMint species (<i>Lamiaceae</i> family) have been used as traditional remedies for the treatment of several diseases. In this work, we aimed to characterize the biological activities of the total phenolic and flavonoid contents of <i>Mentha pulegium</i> L. extracts collected from two different regions of Tunisia. The highest amounts of total phenols (74.45 ± 0.01 mg GAE/g DW), flavonoids (28.87 ± 0.02 mg RE/g DW), and condensed tannins (4.35 ± 0.02 mg CE/g DW) were found in the Bizerte locality. Methanolic leaf extracts were subjected to HPLC-UV analysis in order to identify and quantify the phenolic composition. This technique allowed us to identify seven phenolic compounds: two phenolic acids and five flavonoid compounds, such as eriocitrin, hesperidin, narirutin, luteolin, and isorhoifolin, which were found in both extracts with significant differences between samples collected from the different regions (<i>p</i> < 0.05). Furthermore, our results showed that the methanolic extract from leaves collected from Bizerte had the highest antioxidant activities (DPPH IC<sub>50</sub> value of 16.31 μg/mL and 570.08 μmol Fe<sup>2+</sup>/g, respectively). Both extracts showed high radical-scavenging activity as well as significant antimicrobial activity against eight tested bacteria. The highest antimicrobial activities were observed against Gram-positive bacteria with inhibition zone diameters and MIC values ranging between 19 and 32 mm and 40 and 160 µg/mL, respectively. Interestingly, at 10 μg/mL, the extract had a significant effect on cell proliferation of U87 human glioblastoma cells. These findings open perspectives for the use of <i>Mentha pulegium</i> L. extract in green pharmacy, alternative/complementary medicine, and natural preventive therapies for the development of effective antioxidant, antibacterial, and/or antitumoral drugs.https://www.mdpi.com/1420-3049/27/1/314medicinal plantpolyphenolsantioxidantantiproliferative and antibacterial activities |
| spellingShingle | Jed Jebali Hanene Ghazghazi Chedia Aouadhi Ines ELBini-Dhouib Ridha Ben Salem Najet Srairi-Abid Naziha Marrakchi Ghayth Rigane Tunisian Native <i>Mentha pulegium</i> L. Extracts: Phytochemical Composition and Biological Activities Molecules medicinal plant polyphenols antioxidant antiproliferative and antibacterial activities |
| title | Tunisian Native <i>Mentha pulegium</i> L. Extracts: Phytochemical Composition and Biological Activities |
| title_full | Tunisian Native <i>Mentha pulegium</i> L. Extracts: Phytochemical Composition and Biological Activities |
| title_fullStr | Tunisian Native <i>Mentha pulegium</i> L. Extracts: Phytochemical Composition and Biological Activities |
| title_full_unstemmed | Tunisian Native <i>Mentha pulegium</i> L. Extracts: Phytochemical Composition and Biological Activities |
| title_short | Tunisian Native <i>Mentha pulegium</i> L. Extracts: Phytochemical Composition and Biological Activities |
| title_sort | tunisian native i mentha pulegium i l extracts phytochemical composition and biological activities |
| topic | medicinal plant polyphenols antioxidant antiproliferative and antibacterial activities |
| url | https://www.mdpi.com/1420-3049/27/1/314 |
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