Ultra-High Frequency Ultrasound Imaging of Bowel Wall in Hirschsprung’s Disease—Correlation and Agreement Analyses of Histoanatomy
Hirschsprung’s disease (HD) is characterized by aganglionosis in the bowel wall, requiring resection. Ultra-high frequency ultrasound (UHFUS) imaging of the bowel wall has been suggested to be an instantaneous method of deciding resection length. The aim of this study was to validate UHFUS imaging o...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/13/8/1388 |
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| author | Tebin Hawez Christina Graneli Tobias Erlöv Emilia Gottberg Rodrigo Munoz Mitev Kristine Hagelsteen Maria Evertsson Tomas Jansson Magnus Cinthio Pernilla Stenström |
| author_facet | Tebin Hawez Christina Graneli Tobias Erlöv Emilia Gottberg Rodrigo Munoz Mitev Kristine Hagelsteen Maria Evertsson Tomas Jansson Magnus Cinthio Pernilla Stenström |
| author_sort | Tebin Hawez |
| collection | DOAJ |
| description | Hirschsprung’s disease (HD) is characterized by aganglionosis in the bowel wall, requiring resection. Ultra-high frequency ultrasound (UHFUS) imaging of the bowel wall has been suggested to be an instantaneous method of deciding resection length. The aim of this study was to validate UHFUS imaging of the bowel wall in children with HD by exploring the correlation and systematic differences between UHFUS and histopathology. Resected fresh bowel specimens of children 0–1 years old, operated on for rectosigmoid aganglionosis at a national HD center 2018–2021, were examined ex vivo with UHFUS center frequency 50 MHz. Aganglionosis and ganglionosis were confirmed by histopathological staining and immunohistochemistry. Histoanatomical layers of bowel wall in histopathological and UHFUS images, respectively, were outlined using MATLAB programs. Both histopathological and UHFUS images were available for 19 aganglionic and 18 ganglionic specimens. The thickness of muscularis interna correlated positively between histopathology and UHFUS in both aganglionosis (R = 0.651, <i>p</i> = 0.003) and ganglionosis (R = 0.534, <i>p</i> = 0.023). The muscularis interna was systematically thicker in histopathology than in UHFUS images in both aganglionosis (0.499 vs. 0.309 mm; <i>p</i> < 0.001) and ganglionosis (0.644 versus 0.556 mm; <i>p</i> = 0.003). Significant correlations and systematic differences between histopathological and UHFUS images support the hypothesis that UHFUS reproduces the histoanatomy of the bowel wall in HD accurately. |
| first_indexed | 2024-03-11T05:05:54Z |
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| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T05:05:54Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-48b952ac60ec41e59dee68a51840ef632023-11-17T18:54:25ZengMDPI AGDiagnostics2075-44182023-04-01138138810.3390/diagnostics13081388Ultra-High Frequency Ultrasound Imaging of Bowel Wall in Hirschsprung’s Disease—Correlation and Agreement Analyses of HistoanatomyTebin Hawez0Christina Graneli1Tobias Erlöv2Emilia Gottberg3Rodrigo Munoz Mitev4Kristine Hagelsteen5Maria Evertsson6Tomas Jansson7Magnus Cinthio8Pernilla Stenström9Department of Pediatric Surgery, Children’s Hospital, Skåne University Hospital Lund, Lund University, 221 85 Lund, SwedenDepartment of Pediatric Surgery, Children’s Hospital, Skåne University Hospital Lund, Lund University, 221 85 Lund, SwedenDepartment of Biomedical Engineering, The Faculty of Engineering, Lund University, 223 63 Lund, SwedenDepartment of Clinical Genetics and Pathology, Skåne University Hospital, Lund University, 222 42 Lund, SwedenDepartment of Clinical Genetics and Pathology, Skåne University Hospital, Lund University, 222 42 Lund, SwedenDepartment of Pediatric Surgery, Children’s Hospital, Skåne University Hospital Lund, Lund University, 221 85 Lund, SwedenDepartment of Biomedical Engineering, Skåne University Hospital Lund, The Faculty of Engineering, Lund University, 221 00 Lund, SwedenDepartment of Biomedical Engineering, Skåne University Hospital Lund, The Faculty of Engineering, Lund University, 221 00 Lund, SwedenDepartment of Biomedical Engineering, The Faculty of Engineering, Lund University, 223 63 Lund, SwedenDepartment of Pediatric Surgery, Children’s Hospital, Skåne University Hospital Lund, Lund University, 221 85 Lund, SwedenHirschsprung’s disease (HD) is characterized by aganglionosis in the bowel wall, requiring resection. Ultra-high frequency ultrasound (UHFUS) imaging of the bowel wall has been suggested to be an instantaneous method of deciding resection length. The aim of this study was to validate UHFUS imaging of the bowel wall in children with HD by exploring the correlation and systematic differences between UHFUS and histopathology. Resected fresh bowel specimens of children 0–1 years old, operated on for rectosigmoid aganglionosis at a national HD center 2018–2021, were examined ex vivo with UHFUS center frequency 50 MHz. Aganglionosis and ganglionosis were confirmed by histopathological staining and immunohistochemistry. Histoanatomical layers of bowel wall in histopathological and UHFUS images, respectively, were outlined using MATLAB programs. Both histopathological and UHFUS images were available for 19 aganglionic and 18 ganglionic specimens. The thickness of muscularis interna correlated positively between histopathology and UHFUS in both aganglionosis (R = 0.651, <i>p</i> = 0.003) and ganglionosis (R = 0.534, <i>p</i> = 0.023). The muscularis interna was systematically thicker in histopathology than in UHFUS images in both aganglionosis (0.499 vs. 0.309 mm; <i>p</i> < 0.001) and ganglionosis (0.644 versus 0.556 mm; <i>p</i> = 0.003). Significant correlations and systematic differences between histopathological and UHFUS images support the hypothesis that UHFUS reproduces the histoanatomy of the bowel wall in HD accurately.https://www.mdpi.com/2075-4418/13/8/1388Hirschsprung’s diseasebowel wallultra-high frequency ultrasoundhistopathologychildren |
| spellingShingle | Tebin Hawez Christina Graneli Tobias Erlöv Emilia Gottberg Rodrigo Munoz Mitev Kristine Hagelsteen Maria Evertsson Tomas Jansson Magnus Cinthio Pernilla Stenström Ultra-High Frequency Ultrasound Imaging of Bowel Wall in Hirschsprung’s Disease—Correlation and Agreement Analyses of Histoanatomy Diagnostics Hirschsprung’s disease bowel wall ultra-high frequency ultrasound histopathology children |
| title | Ultra-High Frequency Ultrasound Imaging of Bowel Wall in Hirschsprung’s Disease—Correlation and Agreement Analyses of Histoanatomy |
| title_full | Ultra-High Frequency Ultrasound Imaging of Bowel Wall in Hirschsprung’s Disease—Correlation and Agreement Analyses of Histoanatomy |
| title_fullStr | Ultra-High Frequency Ultrasound Imaging of Bowel Wall in Hirschsprung’s Disease—Correlation and Agreement Analyses of Histoanatomy |
| title_full_unstemmed | Ultra-High Frequency Ultrasound Imaging of Bowel Wall in Hirschsprung’s Disease—Correlation and Agreement Analyses of Histoanatomy |
| title_short | Ultra-High Frequency Ultrasound Imaging of Bowel Wall in Hirschsprung’s Disease—Correlation and Agreement Analyses of Histoanatomy |
| title_sort | ultra high frequency ultrasound imaging of bowel wall in hirschsprung s disease correlation and agreement analyses of histoanatomy |
| topic | Hirschsprung’s disease bowel wall ultra-high frequency ultrasound histopathology children |
| url | https://www.mdpi.com/2075-4418/13/8/1388 |
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