Mitigation of Humic Acid Inhibition in Anaerobic Digestion of Cellulose by Addition of Various Salts
Humic compounds are inhibitory to the anaerobic hydrolysis of cellulosic biomass. In this study, the impact of salt addition to mitigate the inhibitory effects of humic compounds was investigated. The experiment was conducted using batch tests to monitor the anaerobic hydrolysis of cellulose in the...
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MDPI AG
2015-03-01
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| Series: | Bioengineering |
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| Online Access: | http://www.mdpi.com/2306-5354/2/2/54 |
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| author | Samet Azman Ahmad F. Khadem Grietje Zeeman Jules B. van Lier Caroline M. Plugge |
| author_facet | Samet Azman Ahmad F. Khadem Grietje Zeeman Jules B. van Lier Caroline M. Plugge |
| author_sort | Samet Azman |
| collection | DOAJ |
| description | Humic compounds are inhibitory to the anaerobic hydrolysis of cellulosic biomass. In this study, the impact of salt addition to mitigate the inhibitory effects of humic compounds was investigated. The experiment was conducted using batch tests to monitor the anaerobic hydrolysis of cellulose in the presence of humic acid. Sodium, potassium, calcium, magnesium and iron salts were tested separately for their efficiency to mitigate humic acid inhibition. All experiments were done under mesophilic conditions (30 °C) and at pH 7. Methane production was monitored online, using the Automatic Methane Potential Test System. Methane production, soluble chemical oxygen demand and volatile fatty acid content of the samples were measured to calculate the hydrolysis efficiencies. Addition of magnesium, calcium and iron salts clearly mitigated the inhibitory effects of humic acid and hydrolysis efficiencies reached up to 75%, 65% and 72%, respectively, which were similar to control experiments. Conversely, potassium and sodium salts addition did not mitigate the inhibition and hydrolysis efficiencies were found to be less than 40%. Mitigation of humic acid inhibition via salt addition was also validated by inductively coupled plasma atomic emission spectroscopy analyses, which showed the binding capacity of different cations to humic acid. |
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| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T19:13:01Z |
| publishDate | 2015-03-01 |
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| spelling | doaj.art-48ba9bd16eb145edaa4f658c39643d3e2023-08-02T05:43:57ZengMDPI AGBioengineering2306-53542015-03-0122546510.3390/bioengineering2020054bioengineering2020054Mitigation of Humic Acid Inhibition in Anaerobic Digestion of Cellulose by Addition of Various SaltsSamet Azman0Ahmad F. Khadem1Grietje Zeeman2Jules B. van Lier3Caroline M. Plugge4Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The NetherlandsLaboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The NetherlandsSub-Department of Environmental Biotechnology, Wageningen University, 6700 AA Wageningen, The NetherlandsSection Sanitary Engineering, Department of Water Management, Faculty of Civil Engineering and Geosciences, Delft University of Technology, 2600 GA Delft, The NetherlandsLaboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The NetherlandsHumic compounds are inhibitory to the anaerobic hydrolysis of cellulosic biomass. In this study, the impact of salt addition to mitigate the inhibitory effects of humic compounds was investigated. The experiment was conducted using batch tests to monitor the anaerobic hydrolysis of cellulose in the presence of humic acid. Sodium, potassium, calcium, magnesium and iron salts were tested separately for their efficiency to mitigate humic acid inhibition. All experiments were done under mesophilic conditions (30 °C) and at pH 7. Methane production was monitored online, using the Automatic Methane Potential Test System. Methane production, soluble chemical oxygen demand and volatile fatty acid content of the samples were measured to calculate the hydrolysis efficiencies. Addition of magnesium, calcium and iron salts clearly mitigated the inhibitory effects of humic acid and hydrolysis efficiencies reached up to 75%, 65% and 72%, respectively, which were similar to control experiments. Conversely, potassium and sodium salts addition did not mitigate the inhibition and hydrolysis efficiencies were found to be less than 40%. Mitigation of humic acid inhibition via salt addition was also validated by inductively coupled plasma atomic emission spectroscopy analyses, which showed the binding capacity of different cations to humic acid.http://www.mdpi.com/2306-5354/2/2/54celluloseanaerobic digestionhumic acidhydrolysisinhibitionmitigationsalt additioncations |
| spellingShingle | Samet Azman Ahmad F. Khadem Grietje Zeeman Jules B. van Lier Caroline M. Plugge Mitigation of Humic Acid Inhibition in Anaerobic Digestion of Cellulose by Addition of Various Salts Bioengineering cellulose anaerobic digestion humic acid hydrolysis inhibition mitigation salt addition cations |
| title | Mitigation of Humic Acid Inhibition in Anaerobic Digestion of Cellulose by Addition of Various Salts |
| title_full | Mitigation of Humic Acid Inhibition in Anaerobic Digestion of Cellulose by Addition of Various Salts |
| title_fullStr | Mitigation of Humic Acid Inhibition in Anaerobic Digestion of Cellulose by Addition of Various Salts |
| title_full_unstemmed | Mitigation of Humic Acid Inhibition in Anaerobic Digestion of Cellulose by Addition of Various Salts |
| title_short | Mitigation of Humic Acid Inhibition in Anaerobic Digestion of Cellulose by Addition of Various Salts |
| title_sort | mitigation of humic acid inhibition in anaerobic digestion of cellulose by addition of various salts |
| topic | cellulose anaerobic digestion humic acid hydrolysis inhibition mitigation salt addition cations |
| url | http://www.mdpi.com/2306-5354/2/2/54 |
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