Biosynthesis of Fatty Acid Derivatives by Recombinant <i>Yarrowia lipolytica</i> Containing MsexD2 and MsexD3 Desaturase Genes from <i>Manduca sexta</i>
One of the most interesting groups of fatty acid derivates is the group of conjugated fatty acids from which the most researched include: conjugated linoleic acid (CLA) and conjugated linolenic acid (CLNA), which are associated with countless health benefits. Sex pheromone mixtures of some insect sp...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/9/1/114 |
| _version_ | 1827624204333219840 |
|---|---|
| author | Jaroslav Hambalko Peter Gajdoš Jean-Marc Nicaud Rodrigo Ledesma-Amaro Michal Tupec Iva Pichová Milan Čertík |
| author_facet | Jaroslav Hambalko Peter Gajdoš Jean-Marc Nicaud Rodrigo Ledesma-Amaro Michal Tupec Iva Pichová Milan Čertík |
| author_sort | Jaroslav Hambalko |
| collection | DOAJ |
| description | One of the most interesting groups of fatty acid derivates is the group of conjugated fatty acids from which the most researched include: conjugated linoleic acid (CLA) and conjugated linolenic acid (CLNA), which are associated with countless health benefits. Sex pheromone mixtures of some insect species, including tobacco horn-worm (<i>Manduca sexta</i>), are typical for the production of uncommon C16 long conjugated fatty acids with two and three conjugated double bonds, as opposed to C18 long CLA and CLNA. In this study, <i>M. sexta</i> desaturases MsexD2 and MsexD3 were expressed in multiple strains of <i>Y. lipolytica</i> with different genotypes. Experiments with the supplementation of fatty acid methyl esters into the medium resulted in the production of novel fatty acids. Using GCxGC-MS, 20 new fatty acids with two or three double bonds were identified. Fatty acids with conjugated or isolated double bonds, or a combination of both, were produced in trace amounts. The results of this study prove that <i>Y. lipolytica</i> is capable of synthesizing C16-conjugated fatty acids. Further genetic optimization of the <i>Y. lipolytica</i> genome and optimization of the fermentation process could lead to increased production of novel fatty acid derivatives with biotechnologically interesting properties. |
| first_indexed | 2024-03-09T12:03:33Z |
| format | Article |
| id | doaj.art-48bb5ea7646a4e5c832fca28527085cd |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T12:03:33Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-48bb5ea7646a4e5c832fca28527085cd2023-11-30T23:00:31ZengMDPI AGJournal of Fungi2309-608X2023-01-019111410.3390/jof9010114Biosynthesis of Fatty Acid Derivatives by Recombinant <i>Yarrowia lipolytica</i> Containing MsexD2 and MsexD3 Desaturase Genes from <i>Manduca sexta</i>Jaroslav Hambalko0Peter Gajdoš1Jean-Marc Nicaud2Rodrigo Ledesma-Amaro3Michal Tupec4Iva Pichová5Milan Čertík6Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, SlovakiaInstitute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, SlovakiaUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Domaine de Vilvert, 78352 Jouy-en-Josas, FranceDepartment of Bioengineering and Imperial College Centre for Synthetic Biology, Faculty of Engineering, Imperial College, London SW7 2AZ, UKInstitute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo náměstí 542/2, 160 00 Prague 6, Czech RepublicInstitute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo náměstí 542/2, 160 00 Prague 6, Czech RepublicInstitute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, SlovakiaOne of the most interesting groups of fatty acid derivates is the group of conjugated fatty acids from which the most researched include: conjugated linoleic acid (CLA) and conjugated linolenic acid (CLNA), which are associated with countless health benefits. Sex pheromone mixtures of some insect species, including tobacco horn-worm (<i>Manduca sexta</i>), are typical for the production of uncommon C16 long conjugated fatty acids with two and three conjugated double bonds, as opposed to C18 long CLA and CLNA. In this study, <i>M. sexta</i> desaturases MsexD2 and MsexD3 were expressed in multiple strains of <i>Y. lipolytica</i> with different genotypes. Experiments with the supplementation of fatty acid methyl esters into the medium resulted in the production of novel fatty acids. Using GCxGC-MS, 20 new fatty acids with two or three double bonds were identified. Fatty acids with conjugated or isolated double bonds, or a combination of both, were produced in trace amounts. The results of this study prove that <i>Y. lipolytica</i> is capable of synthesizing C16-conjugated fatty acids. Further genetic optimization of the <i>Y. lipolytica</i> genome and optimization of the fermentation process could lead to increased production of novel fatty acid derivatives with biotechnologically interesting properties.https://www.mdpi.com/2309-608X/9/1/114<i>Yarrowia</i>pheromonedesaturase<i>Manduca</i>conjugasemetabolic engineering |
| spellingShingle | Jaroslav Hambalko Peter Gajdoš Jean-Marc Nicaud Rodrigo Ledesma-Amaro Michal Tupec Iva Pichová Milan Čertík Biosynthesis of Fatty Acid Derivatives by Recombinant <i>Yarrowia lipolytica</i> Containing MsexD2 and MsexD3 Desaturase Genes from <i>Manduca sexta</i> Journal of Fungi <i>Yarrowia</i> pheromone desaturase <i>Manduca</i> conjugase metabolic engineering |
| title | Biosynthesis of Fatty Acid Derivatives by Recombinant <i>Yarrowia lipolytica</i> Containing MsexD2 and MsexD3 Desaturase Genes from <i>Manduca sexta</i> |
| title_full | Biosynthesis of Fatty Acid Derivatives by Recombinant <i>Yarrowia lipolytica</i> Containing MsexD2 and MsexD3 Desaturase Genes from <i>Manduca sexta</i> |
| title_fullStr | Biosynthesis of Fatty Acid Derivatives by Recombinant <i>Yarrowia lipolytica</i> Containing MsexD2 and MsexD3 Desaturase Genes from <i>Manduca sexta</i> |
| title_full_unstemmed | Biosynthesis of Fatty Acid Derivatives by Recombinant <i>Yarrowia lipolytica</i> Containing MsexD2 and MsexD3 Desaturase Genes from <i>Manduca sexta</i> |
| title_short | Biosynthesis of Fatty Acid Derivatives by Recombinant <i>Yarrowia lipolytica</i> Containing MsexD2 and MsexD3 Desaturase Genes from <i>Manduca sexta</i> |
| title_sort | biosynthesis of fatty acid derivatives by recombinant i yarrowia lipolytica i containing msexd2 and msexd3 desaturase genes from i manduca sexta i |
| topic | <i>Yarrowia</i> pheromone desaturase <i>Manduca</i> conjugase metabolic engineering |
| url | https://www.mdpi.com/2309-608X/9/1/114 |
| work_keys_str_mv | AT jaroslavhambalko biosynthesisoffattyacidderivativesbyrecombinantiyarrowialipolyticaicontainingmsexd2andmsexd3desaturasegenesfromimanducasextai AT petergajdos biosynthesisoffattyacidderivativesbyrecombinantiyarrowialipolyticaicontainingmsexd2andmsexd3desaturasegenesfromimanducasextai AT jeanmarcnicaud biosynthesisoffattyacidderivativesbyrecombinantiyarrowialipolyticaicontainingmsexd2andmsexd3desaturasegenesfromimanducasextai AT rodrigoledesmaamaro biosynthesisoffattyacidderivativesbyrecombinantiyarrowialipolyticaicontainingmsexd2andmsexd3desaturasegenesfromimanducasextai AT michaltupec biosynthesisoffattyacidderivativesbyrecombinantiyarrowialipolyticaicontainingmsexd2andmsexd3desaturasegenesfromimanducasextai AT ivapichova biosynthesisoffattyacidderivativesbyrecombinantiyarrowialipolyticaicontainingmsexd2andmsexd3desaturasegenesfromimanducasextai AT milancertik biosynthesisoffattyacidderivativesbyrecombinantiyarrowialipolyticaicontainingmsexd2andmsexd3desaturasegenesfromimanducasextai |