Detection of antibiotic resistance and capsular types of group B streptococci isolated from vaginal swabs of pregnant women
Introduction: Group B streptococcus (GBS) is an opportunistic pathogen that colonizes the gastrointestinal and genital tract of 10 - 30% of healthy people. It can cause early or late-onset neonatal disease manifested as pneumonia, sepsis, and meningitis. Penicillins are the first-choice therapy and...
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| Format: | Article |
| Language: | English |
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University of Belgrade, Medical Faculty
2023-01-01
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| Series: | Medicinski Podmladak |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2023/0369-15272305050P.pdf |
| _version_ | 1797223715619995648 |
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| author | Paunović Bojana Kekić Dušan Kabić Jovana Gajić Ina Jovićević Miloš |
| author_facet | Paunović Bojana Kekić Dušan Kabić Jovana Gajić Ina Jovićević Miloš |
| author_sort | Paunović Bojana |
| collection | DOAJ |
| description | Introduction: Group B streptococcus (GBS) is an opportunistic pathogen that colonizes the gastrointestinal and genital tract of 10 - 30% of healthy people. It can cause early or late-onset neonatal disease manifested as pneumonia, sepsis, and meningitis. Penicillins are the first-choice therapy and prophylaxis of mother-to-child transmission. In the case of penicillin allergy, macrolides or vancomycin are used. Aim: The objectives of study were to determine antimicrobial susceptibility and macrolides resistance phenotypes of GBS isolates, as well as to determine capsular types (CPS) and detection of hypervirulent ST17 clone. Material and methods: From January 1st to December 31st 2021, 134 GBS isolates were collected from vaginal swabs of pregnant women. Identification was performed using conventional microbiological methods and molecular detection of species-specific dltR gene. The disk diffusion method was used to test antibiotic resistance according to EUCAST standards. Capsular typing was conducted by multiplex PCR method. A hypervirulent ST17 clone was identified by the detection of the hvgA gene using PCR. Results: All isolates were susceptible to penicillin, vancomycin, and fluoroquinolones. Tetracycline resistance was the most common, detected in 81.3% (109/134) strains. Macrolide resistance was detected in 3.3% (50/134) of isolates, while 14.9% (20/134) showed resistance to high doses of gentamicin. Resistance to chloramphenicol was noticed in 1.5% (2/134) isolates. The most common macrolide resistance phenotype was cMLS (31/50, 62.0%). The most identified CPS were V found in 33 isolates (25.0%) and III identified in 30 strains (22.0%). A hypervirulent ST17 clone was detected in 12.7% (17/134) of isolates; 15 belonged to CPS III and 2 to CPS IV. Conclusion: The high frequency of macrolide resistance in group B streptococci isolated from colonized pregnant women is a severe public health concern. Also, the detection of numerous CPS III isolates and ST17 clones indicates the need for continuous screening and monitoring of the prevalence of GBS infections in pregnant women and neonates. |
| first_indexed | 2024-04-24T13:41:38Z |
| format | Article |
| id | doaj.art-48bc68fde8fe4a4d816be437ef958b9b |
| institution | Directory Open Access Journal |
| issn | 0369-1527 2466-5525 |
| language | English |
| last_indexed | 2024-04-24T13:41:38Z |
| publishDate | 2023-01-01 |
| publisher | University of Belgrade, Medical Faculty |
| record_format | Article |
| series | Medicinski Podmladak |
| spelling | doaj.art-48bc68fde8fe4a4d816be437ef958b9b2024-04-04T08:28:43ZengUniversity of Belgrade, Medical FacultyMedicinski Podmladak0369-15272466-55252023-01-01745505510.5937/mp74-392520369-15272305050PDetection of antibiotic resistance and capsular types of group B streptococci isolated from vaginal swabs of pregnant womenPaunović Bojana0Kekić Dušan1Kabić Jovana2Gajić Ina3https://orcid.org/0000-0002-1578-2776Jovićević Miloš4Univerzitet u Beogradu, Medicinski fakultet, Beograd, SerbiaUniverzitet u Beogradu, Medicinski fakultet, Institut za mikrobiologiju i imunologiju, Beograd, SerbiaUniverzitet u Beogradu, Medicinski fakultet, Institut za mikrobiologiju i imunologiju, Beograd, SerbiaUniverzitet u Beogradu, Medicinski fakultet, Institut za mikrobiologiju i imunologiju, Beograd, SerbiaUniverzitet u Beogradu, Medicinski fakultet, Institut za mikrobiologiju i imunologiju, Beograd, SerbiaIntroduction: Group B streptococcus (GBS) is an opportunistic pathogen that colonizes the gastrointestinal and genital tract of 10 - 30% of healthy people. It can cause early or late-onset neonatal disease manifested as pneumonia, sepsis, and meningitis. Penicillins are the first-choice therapy and prophylaxis of mother-to-child transmission. In the case of penicillin allergy, macrolides or vancomycin are used. Aim: The objectives of study were to determine antimicrobial susceptibility and macrolides resistance phenotypes of GBS isolates, as well as to determine capsular types (CPS) and detection of hypervirulent ST17 clone. Material and methods: From January 1st to December 31st 2021, 134 GBS isolates were collected from vaginal swabs of pregnant women. Identification was performed using conventional microbiological methods and molecular detection of species-specific dltR gene. The disk diffusion method was used to test antibiotic resistance according to EUCAST standards. Capsular typing was conducted by multiplex PCR method. A hypervirulent ST17 clone was identified by the detection of the hvgA gene using PCR. Results: All isolates were susceptible to penicillin, vancomycin, and fluoroquinolones. Tetracycline resistance was the most common, detected in 81.3% (109/134) strains. Macrolide resistance was detected in 3.3% (50/134) of isolates, while 14.9% (20/134) showed resistance to high doses of gentamicin. Resistance to chloramphenicol was noticed in 1.5% (2/134) isolates. The most common macrolide resistance phenotype was cMLS (31/50, 62.0%). The most identified CPS were V found in 33 isolates (25.0%) and III identified in 30 strains (22.0%). A hypervirulent ST17 clone was detected in 12.7% (17/134) of isolates; 15 belonged to CPS III and 2 to CPS IV. Conclusion: The high frequency of macrolide resistance in group B streptococci isolated from colonized pregnant women is a severe public health concern. Also, the detection of numerous CPS III isolates and ST17 clones indicates the need for continuous screening and monitoring of the prevalence of GBS infections in pregnant women and neonates.https://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2023/0369-15272305050P.pdfgroup b streptococcusresistancecapsular distributionclone st17 |
| spellingShingle | Paunović Bojana Kekić Dušan Kabić Jovana Gajić Ina Jovićević Miloš Detection of antibiotic resistance and capsular types of group B streptococci isolated from vaginal swabs of pregnant women Medicinski Podmladak group b streptococcus resistance capsular distribution clone st17 |
| title | Detection of antibiotic resistance and capsular types of group B streptococci isolated from vaginal swabs of pregnant women |
| title_full | Detection of antibiotic resistance and capsular types of group B streptococci isolated from vaginal swabs of pregnant women |
| title_fullStr | Detection of antibiotic resistance and capsular types of group B streptococci isolated from vaginal swabs of pregnant women |
| title_full_unstemmed | Detection of antibiotic resistance and capsular types of group B streptococci isolated from vaginal swabs of pregnant women |
| title_short | Detection of antibiotic resistance and capsular types of group B streptococci isolated from vaginal swabs of pregnant women |
| title_sort | detection of antibiotic resistance and capsular types of group b streptococci isolated from vaginal swabs of pregnant women |
| topic | group b streptococcus resistance capsular distribution clone st17 |
| url | https://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2023/0369-15272305050P.pdf |
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