Comparison of Human Milk Immunoglobulin Survival during Gastric Digestion between Preterm and Term Infants
Human milk provides immunoglobulins (Igs) that supplement the passive immune system of neonates; however, the extent of survival of these Igs during gastric digestion and whether this differs between preterm and term infants remains unknown. Human milk, and infant gastric samples at 2 h post-ingesti...
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MDPI AG
2018-05-01
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| Series: | Nutrients |
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| Online Access: | http://www.mdpi.com/2072-6643/10/5/631 |
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| author | Veronique Demers-Mathieu Mark A. Underwood Robert L. Beverly Søren D. Nielsen David C. Dallas |
| author_facet | Veronique Demers-Mathieu Mark A. Underwood Robert L. Beverly Søren D. Nielsen David C. Dallas |
| author_sort | Veronique Demers-Mathieu |
| collection | DOAJ |
| description | Human milk provides immunoglobulins (Igs) that supplement the passive immune system of neonates; however, the extent of survival of these Igs during gastric digestion and whether this differs between preterm and term infants remains unknown. Human milk, and infant gastric samples at 2 h post-ingestion were collected from 15 preterm (23–32 week gestational age (GA)) mother-infant pairs and from 8 term (38–40 week of GA) mother-infant pairs within 7–98 days postnatal age. Samples were analyzed via ELISA for concentration of total IgA (secretory IgA (SIgA)/IgA), total secretory component (SC/SIgA/SIgM), total IgM (SIgM/IgM), and IgG as well as peptidomics. Total IgA concentration decreased by 60% from human milk to the preterm infant stomach and decreased by 48% in the term infant stomach. Total IgM and IgG concentrations decreased by 33% and 77%, respectively, from human milk to the term infant stomach but were stable in the preterm infant stomach. Release of peptides from all Ig isotypes in the term infant stomach was higher than in the preterm stomach. Overall, the stability of human milk Igs during gastric digestion is higher in preterm infant than in term infants, which could be beneficial for assisting the preterm infants’ immature immune system. |
| first_indexed | 2024-04-12T06:02:37Z |
| format | Article |
| id | doaj.art-48bcadf995c04e869e55f7a00e427556 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-04-12T06:02:37Z |
| publishDate | 2018-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-48bcadf995c04e869e55f7a00e4275562022-12-22T03:45:00ZengMDPI AGNutrients2072-66432018-05-0110563110.3390/nu10050631nu10050631Comparison of Human Milk Immunoglobulin Survival during Gastric Digestion between Preterm and Term InfantsVeronique Demers-Mathieu0Mark A. Underwood1Robert L. Beverly2Søren D. Nielsen3David C. Dallas4Nutrition Program, School of Biological and Population Health Sciences, College of Public Health and Human Sciences, Oregon State University, Corvallis, OR 97331, USADepartment of Pediatrics, University of California, Davis, Sacramento, CA 95817, USANutrition Program, School of Biological and Population Health Sciences, College of Public Health and Human Sciences, Oregon State University, Corvallis, OR 97331, USANutrition Program, School of Biological and Population Health Sciences, College of Public Health and Human Sciences, Oregon State University, Corvallis, OR 97331, USANutrition Program, School of Biological and Population Health Sciences, College of Public Health and Human Sciences, Oregon State University, Corvallis, OR 97331, USAHuman milk provides immunoglobulins (Igs) that supplement the passive immune system of neonates; however, the extent of survival of these Igs during gastric digestion and whether this differs between preterm and term infants remains unknown. Human milk, and infant gastric samples at 2 h post-ingestion were collected from 15 preterm (23–32 week gestational age (GA)) mother-infant pairs and from 8 term (38–40 week of GA) mother-infant pairs within 7–98 days postnatal age. Samples were analyzed via ELISA for concentration of total IgA (secretory IgA (SIgA)/IgA), total secretory component (SC/SIgA/SIgM), total IgM (SIgM/IgM), and IgG as well as peptidomics. Total IgA concentration decreased by 60% from human milk to the preterm infant stomach and decreased by 48% in the term infant stomach. Total IgM and IgG concentrations decreased by 33% and 77%, respectively, from human milk to the term infant stomach but were stable in the preterm infant stomach. Release of peptides from all Ig isotypes in the term infant stomach was higher than in the preterm stomach. Overall, the stability of human milk Igs during gastric digestion is higher in preterm infant than in term infants, which could be beneficial for assisting the preterm infants’ immature immune system.http://www.mdpi.com/2072-6643/10/5/631passive immunityantibodieslactationpeptidomicsprematurityproteolysisbreast milk |
| spellingShingle | Veronique Demers-Mathieu Mark A. Underwood Robert L. Beverly Søren D. Nielsen David C. Dallas Comparison of Human Milk Immunoglobulin Survival during Gastric Digestion between Preterm and Term Infants Nutrients passive immunity antibodies lactation peptidomics prematurity proteolysis breast milk |
| title | Comparison of Human Milk Immunoglobulin Survival during Gastric Digestion between Preterm and Term Infants |
| title_full | Comparison of Human Milk Immunoglobulin Survival during Gastric Digestion between Preterm and Term Infants |
| title_fullStr | Comparison of Human Milk Immunoglobulin Survival during Gastric Digestion between Preterm and Term Infants |
| title_full_unstemmed | Comparison of Human Milk Immunoglobulin Survival during Gastric Digestion between Preterm and Term Infants |
| title_short | Comparison of Human Milk Immunoglobulin Survival during Gastric Digestion between Preterm and Term Infants |
| title_sort | comparison of human milk immunoglobulin survival during gastric digestion between preterm and term infants |
| topic | passive immunity antibodies lactation peptidomics prematurity proteolysis breast milk |
| url | http://www.mdpi.com/2072-6643/10/5/631 |
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