Mapping QTLs for stomatal density and size under drought stress in wheat (Triticum aestivum L.)
Stomatal density and size affect plant water use efficiency, photosynthsis rate and yield. The objective of this study was to gain insights into the variation and genetic basis of stomatal density and size during grain filling under drought stress (DS) and well-watered (WW) conditions. The doubled h...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-09-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915612643 |
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| author | Shu-guang WANG Shou-shan JIA Dai-zhen SUN Hua FAN Xiao-ping CHANG Rui-lian JING |
| author_facet | Shu-guang WANG Shou-shan JIA Dai-zhen SUN Hua FAN Xiao-ping CHANG Rui-lian JING |
| author_sort | Shu-guang WANG |
| collection | DOAJ |
| description | Stomatal density and size affect plant water use efficiency, photosynthsis rate and yield. The objective of this study was to gain insights into the variation and genetic basis of stomatal density and size during grain filling under drought stress (DS) and well-watered (WW) conditions. The doubled haploid population derived from a cross of wheat cultivars Hanxuan 10 (H10), a female parent, and Lumai 14 (L14), a male parent, was used for phenotyping at the heading, flowering, and mid- and late grain filling stages along with established amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. The stomatal density of doubled haploid (DH) lines was gradually increased, while the stomatal lengths and widths were gradually decreased during grain filling stage. Twenty additive QTLs and 19 pairs of epistatic QTLs for the 3 traits were identified under DS. The other 20 QTLs and 25 pairs epistatic QTLs were obtained under WW. Most QTLs made more than 10% contributions to the total phenotypic variations at one growth stage under DS or WW. Furthermore, QTLs for stomatal density near Xwmc74 and Xgwm291 located on chromosome 5A were tightly linked to previously reported QTLs regulating total number of spikelets per spike, number of sterile spikelets per spike and proportion of fertile spikelets per spike. Qsw-2D-1 was detected across stages, and was in the same marker region as a major QTL for plant height, QPH. cgb-2D.1. These indicate that these QTLs on chromosomes 5A and 2D are involved in regulating these agronomic traits and are valuable for molecular breeding. |
| first_indexed | 2024-12-18T01:18:08Z |
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| id | doaj.art-48bd489073cd4636b4d4fe560d6333f1 |
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| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-18T01:18:08Z |
| publishDate | 2016-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-48bd489073cd4636b4d4fe560d6333f12022-12-21T21:25:54ZengElsevierJournal of Integrative Agriculture2095-31192016-09-0115919551967Mapping QTLs for stomatal density and size under drought stress in wheat (Triticum aestivum L.)Shu-guang WANG0Shou-shan JIA1Dai-zhen SUN2Hua FAN3Xiao-ping CHANG4Rui-lian JING5College of Agronomy, Shanxi Agricultural University, Taigu 030801, P.R.ChinaCollege of Agronomy, Shanxi Agricultural University, Taigu 030801, P.R.ChinaCollege of Agronomy, Shanxi Agricultural University, Taigu 030801, P.R.China; Correspondence SUN Dai-zhen, Tel/Fax: +86-354-6288706College of Agronomy, Shanxi Agricultural University, Taigu 030801, P.R.ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China; JING Rui-lian, Tel/Fax: +86-10-82105829Stomatal density and size affect plant water use efficiency, photosynthsis rate and yield. The objective of this study was to gain insights into the variation and genetic basis of stomatal density and size during grain filling under drought stress (DS) and well-watered (WW) conditions. The doubled haploid population derived from a cross of wheat cultivars Hanxuan 10 (H10), a female parent, and Lumai 14 (L14), a male parent, was used for phenotyping at the heading, flowering, and mid- and late grain filling stages along with established amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. The stomatal density of doubled haploid (DH) lines was gradually increased, while the stomatal lengths and widths were gradually decreased during grain filling stage. Twenty additive QTLs and 19 pairs of epistatic QTLs for the 3 traits were identified under DS. The other 20 QTLs and 25 pairs epistatic QTLs were obtained under WW. Most QTLs made more than 10% contributions to the total phenotypic variations at one growth stage under DS or WW. Furthermore, QTLs for stomatal density near Xwmc74 and Xgwm291 located on chromosome 5A were tightly linked to previously reported QTLs regulating total number of spikelets per spike, number of sterile spikelets per spike and proportion of fertile spikelets per spike. Qsw-2D-1 was detected across stages, and was in the same marker region as a major QTL for plant height, QPH. cgb-2D.1. These indicate that these QTLs on chromosomes 5A and 2D are involved in regulating these agronomic traits and are valuable for molecular breeding.http://www.sciencedirect.com/science/article/pii/S2095311915612643wheat (Triticum aestivum L.)stomatal densitystomatal lengthstomatal widthquantitative trait loci |
| spellingShingle | Shu-guang WANG Shou-shan JIA Dai-zhen SUN Hua FAN Xiao-ping CHANG Rui-lian JING Mapping QTLs for stomatal density and size under drought stress in wheat (Triticum aestivum L.) Journal of Integrative Agriculture wheat (Triticum aestivum L.) stomatal density stomatal length stomatal width quantitative trait loci |
| title | Mapping QTLs for stomatal density and size under drought stress in wheat (Triticum aestivum L.) |
| title_full | Mapping QTLs for stomatal density and size under drought stress in wheat (Triticum aestivum L.) |
| title_fullStr | Mapping QTLs for stomatal density and size under drought stress in wheat (Triticum aestivum L.) |
| title_full_unstemmed | Mapping QTLs for stomatal density and size under drought stress in wheat (Triticum aestivum L.) |
| title_short | Mapping QTLs for stomatal density and size under drought stress in wheat (Triticum aestivum L.) |
| title_sort | mapping qtls for stomatal density and size under drought stress in wheat triticum aestivum l |
| topic | wheat (Triticum aestivum L.) stomatal density stomatal length stomatal width quantitative trait loci |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915612643 |
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