Whole Genome Sequencing Analysis of Effects of CRISPR/Cas9 in <i>Komagataella phaffii</i>: A Budding Yeast in Distress

Whole Genome Sequencing Analysis of Effects of CRISPR/Cas9 in <i>Komagataella phaffii</i>: A Budding Yeast in Distress

The industrially important non-conventional yeast <i>Komagataella phaffii</i> suffers from low rates of homologous recombination, making site specific genetic engineering tedious. Therefore, genome editing using CRISPR/Cas represents a simple and efficient alternative. To characterize on...

Full description

Bibliographic Details
Main Authors:	Veronika Schusterbauer, Jasmin E. Fischer, Sarah Gangl, Lisa Schenzle, Claudia Rinnofner, Martina Geier, Christian Sailer, Anton Glieder, Gerhard G. Thallinger
Format:	Article
Language:	English
Published:	MDPI AG 2022-09-01
Series:	Journal of Fungi
Subjects:	CRISPR/Cas technology non-conventional yeasts genome analysis non-homologous end joining
Online Access:	https://www.mdpi.com/2309-608X/8/10/992

Similar Items

Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions in <i>Komagataella phaffii</i>
by: Kirill Smirnov, et al.
Published: (2024-03-01)

A high-throughput small molecule screen identifies farrerol as a potentiator of CRISPR/Cas9-mediated genome editing
by: Weina Zhang, et al.
Published: (2020-07-01)

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery
by: Steven Lin, et al.
Published: (2014-12-01)

Assessing spacer acquisition rates in E. coli type I-E CRISPR arrays
by: Luke J. Peach, et al.
Published: (2025-01-01)

A Simple and Efficient CRISPR Technique for Protein Tagging
by: Fanning Zeng, et al.
Published: (2020-12-01)

Latest Developed Strategies to Minimize the Off-Target Effects in CRISPR-Cas-Mediated Genome Editing
by: Muhammad Naeem, et al.
Published: (2020-07-01)

A Novel Breakthrough in Leptospira spp. Mutagenesis: Knockout by Combination of CRISPR/Cas9 and Non-homologous End-Joining Systems
by: Luis G. V. Fernandes, et al.
Published: (2022-05-01)

Investigating The Correction of IVS II-1 (G> A) Mutation in HBB Gene in TLS-12 Cell Line Using CRISPR/Cas9 System
by: Nazli Servatian, et al.
Published: (2023-03-01)

Saccharomyces cerevisiae Rev7 promotes non-homologous end-joining by blocking Mre11 nuclease and Rad50’s ATPase activities and homologous recombination
by: Sugith Badugu, et al.
Published: (2024-12-01)

Functional Roles of Homologous Recombination and Non-Homologous End Joining in DNA Damage Response and Microevolution in <i>Cryptococcus neoformans</i>
by: Kwang-Woo Jung, et al.
Published: (2021-07-01)

Prime Editing: Mechanistic Insights and DNA Repair Modulation
by: Astrid Mentani, et al.
Published: (2025-02-01)

Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free PDH promoter
by: Núria Bernat-Camps, et al.
Published: (2023-03-01)

Development of a Komagataella phaffii cell factory for sustainable production of ( +)-valencene
by: Jintao Cheng, et al.
Published: (2025-01-01)

Cadmium chloride down-regulates the expression of Rad51 in HC11 cells and reduces knock-in efficiency
by: Ga-Yeon Kim, et al.
Published: (2023-09-01)

Predictable NHEJ Insertion and Assessment of HDR Editing Strategies in Plants
by: Kutubuddin A. Molla, et al.
Published: (2022-03-01)

Characterization of DNA damage repair pathway utilization in high-grade serous ovarian cancers yields rational therapeutic approaches
by: Erika Nakatsuka, et al.
Published: (2024-12-01)

Loss of tet methyl cytosine dioxygenase 3 (TET3) enhances cardiac fibrosis via modulating the DNA damage repair response
by: Sandip Kumar Rath, et al.
Published: (2024-08-01)

Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in <i>Komagataella phaffii</i>
by: Dalvie, Neil C., et al.
Published: (2022)

Molecular Process Producing Oncogene Fusion in Lung Cancer Cells by Illegitimate Repair of DNA Double-Strand Breaks
by: Yoshitaka Seki, et al.
Published: (2015-09-01)

Chances and drawbacks of derepressed recombinant enzyme production in continuous cultivations with Komagataella phaffii
by: Mihail Besleaga, et al.
Published: (2025-03-01)

Genomic characterization of IDH-mutant astrocytoma progression to grade 4 in the treatment setting
by: Kirsi J. Rautajoki, et al.
Published: (2023-11-01)

Improving the homologous recombination efficiency of Yarrowia lipolytica by grafting heterologous component from Saccharomyces cerevisiae
by: Qingchun Ji, et al.
Published: (2020-12-01)

Tanshinone I suppresses hepatocellular carcinoma cells growth through targeting DNA double-strand break repair
by: Zhen Qian, et al.
Published: (2023-12-01)

Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene
by: Yinghang Liu, et al.
Published: (2019-12-01)

The decrease in Rad51 and DNA ligase IV nuclear protein expression in Msh2 knockdown HC11 cells induced the low CRISPR/Cas9-mediated knock-in efficiency at the β-casein gene locus
by: Ga-Yeon Kim, et al.
Published: (2025-03-01)

Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks
by: Baolei Yuan, et al.
Published: (2024-04-01)

NHEJ and HDR can occur simultaneously during gene integration into the genome of Aspergillus niger
by: Susanne Fritsche, et al.
Published: (2024-08-01)

Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii
by: Dalvie, Neil C, et al.
Published: (2021)

Bi-directionalized promoter systems allow methanol-free production of hard-to-express peroxygenases with Komagataella Phaffii
by: Mihail Besleaga, et al.
Published: (2024-06-01)

Removal of extra sequences with I-SceI in combination with CRISPR/Cas9 technique for precise gene editing in Drosophila
by: Nikolay Zolotarev, et al.
Published: (2019-04-01)

The role of DNA double-strand break repair defects in the pathogenesis of neurodegenerative diseases
by: Jie Xinyu, et al.
Published: (2023-06-01)

Effect of Pyruvate Decarboxylase Knockout on Product Distribution Using Pichia pastoris (Komagataella phaffii) Engineered for Lactic Acid Production
by: Nadiele T. M. Melo, et al.
Published: (2018-02-01)

Effect of Methionine on Gene Expression in <i>Komagataella phaffii</i> Cells
by: Tatiana Ianshina, et al.
Published: (2023-03-01)

Research progress of DNA damage repair and its effect on perioperative cognitive dysfunction
by: RONG Yu-lan, SHI Cheng-mei, GUO Xiang-yang
Published: (2022-04-01)

Non-Homologous End-Joining Pathway Genotypes Significantly Associated with Nasopharyngeal Carcinoma Susceptibility
by: Chia-Wen Tsai, et al.
Published: (2023-06-01)

Xrcc5/KU80 is not required for the survival or activation of prophase-arrested oocytes in primordial follicles
by: Natasha D. Ratnayaka-Gamage, et al.
Published: (2023-10-01)

Negative feedback loop in the activation of non-homologous end joining DNA repair pathway in Helicobacter pylori infected patients with gastritis
by: Mohsen Amirnorouzi, et al.
Published: (2024-10-01)

Exploiting DNA Endonucleases to Advance Mechanisms of DNA Repair
by: Marlo K. Thompson, et al.
Published: (2021-06-01)

BRCA1 interactors, RAD50 and BRIP1, as prognostic markers for triple-negative breast cancer severity
by: Muhseena N. Katheeja, et al.
Published: (2023-02-01)

DNA double-strand break genetic variants in patients with premature ovarian insufficiency
by: Xuechun Ding, et al.
Published: (2023-07-01)