Multifaceted analyses reveal carbohydrate metabolism mainly affecting the quality of postharvest bamboo shoots
Bamboo shoot is one of nutritious vegetables in China. However, the edible quality of fresh bamboo shoots deteriorates easily after harvest. Here, morphological, physiological, transcriptomic and microRNA sequencing analyses were conducted to investigate the postharvest characteristics of moso bambo...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1021161/full |
| _version_ | 1811271408644259840 |
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| author | Zhen Li Zhen Li Xiurong Xu Xiurong Xu Xiurong Xu Kebin Yang Kebin Yang Chenglei Zhu Chenglei Zhu Yan Liu Yan Liu Zhimin Gao Zhimin Gao |
| author_facet | Zhen Li Zhen Li Xiurong Xu Xiurong Xu Xiurong Xu Kebin Yang Kebin Yang Chenglei Zhu Chenglei Zhu Yan Liu Yan Liu Zhimin Gao Zhimin Gao |
| author_sort | Zhen Li |
| collection | DOAJ |
| description | Bamboo shoot is one of nutritious vegetables in China. However, the edible quality of fresh bamboo shoots deteriorates easily after harvest. Here, morphological, physiological, transcriptomic and microRNA sequencing analyses were conducted to investigate the postharvest characteristics of moso bamboo (Phyllostachys edulis) shoots. Rapid decreases of soluble sugars, structural polysaccharides and hydrolyzed tannins, and increases of lignin and condensed tannins were observed in the postharvest bamboo shoots. Differentially expressed genes (DEGs) and miRNAs with opposite trends were mainly enriched in structural polysaccharide metabolism, starch and sucrose metabolism and glycolysis pathways, which were consistent with the changes of carbohydrates. A co-expression network of carbohydrate metabolism was constructed, which was verified by qPCR and yeast one-hybrid (Y1H) assay. Furthermore, the function of one hub glycosyltransferase gene was validated in Arabidopsis, which confirmed that it was involved in xylan biosynthesis. These results are of great significance for revealing the carbohydrate metabolism mechanisms of postharvest bamboo shoots and provide a potential candidate gene for molecular breeding related to xylan in the future. |
| first_indexed | 2024-04-12T22:19:33Z |
| format | Article |
| id | doaj.art-48c31f91a0f94188a054f8cf10b05117 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T22:19:33Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-48c31f91a0f94188a054f8cf10b051172022-12-22T03:14:24ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.10211611021161Multifaceted analyses reveal carbohydrate metabolism mainly affecting the quality of postharvest bamboo shootsZhen Li0Zhen Li1Xiurong Xu2Xiurong Xu3Xiurong Xu4Kebin Yang5Kebin Yang6Chenglei Zhu7Chenglei Zhu8Yan Liu9Yan Liu10Zhimin Gao11Zhimin Gao12International Centre for Bamboo and Rattan, Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, Beijing, ChinaKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, Beijing, ChinaInternational Centre for Bamboo and Rattan, Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, Beijing, ChinaKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, Beijing, ChinaZhejiang Academy of Forestry, Hangzhou, ChinaInternational Centre for Bamboo and Rattan, Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, Beijing, ChinaKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, Beijing, ChinaInternational Centre for Bamboo and Rattan, Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, Beijing, ChinaKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, Beijing, ChinaInternational Centre for Bamboo and Rattan, Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, Beijing, ChinaKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, Beijing, ChinaInternational Centre for Bamboo and Rattan, Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, Beijing, ChinaKey Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, Beijing, ChinaBamboo shoot is one of nutritious vegetables in China. However, the edible quality of fresh bamboo shoots deteriorates easily after harvest. Here, morphological, physiological, transcriptomic and microRNA sequencing analyses were conducted to investigate the postharvest characteristics of moso bamboo (Phyllostachys edulis) shoots. Rapid decreases of soluble sugars, structural polysaccharides and hydrolyzed tannins, and increases of lignin and condensed tannins were observed in the postharvest bamboo shoots. Differentially expressed genes (DEGs) and miRNAs with opposite trends were mainly enriched in structural polysaccharide metabolism, starch and sucrose metabolism and glycolysis pathways, which were consistent with the changes of carbohydrates. A co-expression network of carbohydrate metabolism was constructed, which was verified by qPCR and yeast one-hybrid (Y1H) assay. Furthermore, the function of one hub glycosyltransferase gene was validated in Arabidopsis, which confirmed that it was involved in xylan biosynthesis. These results are of great significance for revealing the carbohydrate metabolism mechanisms of postharvest bamboo shoots and provide a potential candidate gene for molecular breeding related to xylan in the future.https://www.frontiersin.org/articles/10.3389/fpls.2022.1021161/fullPhyllostachys edulis shootpostharvest physiologycarbohydrate metabolismco-expression networkxylan biosynthesis |
| spellingShingle | Zhen Li Zhen Li Xiurong Xu Xiurong Xu Xiurong Xu Kebin Yang Kebin Yang Chenglei Zhu Chenglei Zhu Yan Liu Yan Liu Zhimin Gao Zhimin Gao Multifaceted analyses reveal carbohydrate metabolism mainly affecting the quality of postharvest bamboo shoots Frontiers in Plant Science Phyllostachys edulis shoot postharvest physiology carbohydrate metabolism co-expression network xylan biosynthesis |
| title | Multifaceted analyses reveal carbohydrate metabolism mainly affecting the quality of postharvest bamboo shoots |
| title_full | Multifaceted analyses reveal carbohydrate metabolism mainly affecting the quality of postharvest bamboo shoots |
| title_fullStr | Multifaceted analyses reveal carbohydrate metabolism mainly affecting the quality of postharvest bamboo shoots |
| title_full_unstemmed | Multifaceted analyses reveal carbohydrate metabolism mainly affecting the quality of postharvest bamboo shoots |
| title_short | Multifaceted analyses reveal carbohydrate metabolism mainly affecting the quality of postharvest bamboo shoots |
| title_sort | multifaceted analyses reveal carbohydrate metabolism mainly affecting the quality of postharvest bamboo shoots |
| topic | Phyllostachys edulis shoot postharvest physiology carbohydrate metabolism co-expression network xylan biosynthesis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1021161/full |
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