| Summary: | <p>Abstract</p> <p>Background</p> <p>The combined sequences encoding a partial and putative <it>rpsI </it>open reading frame (ORF), non-coding (NC) region, a putative ORF for the <it>Campylobacter </it>adhesin to fibronectin-like protein (<it>cadF</it>), a putative Cla_0387 ORF, NC region and a partial and putative Cla_0388 ORF, were identified in 16 <it>Campylobacter lari </it>isolates, using two novel degenerate primer pairs. Probable consensus sequence at the -35 and -10 regions were identified in all <it>C. lari </it>isolates, as a promoter.</p> <p>Results</p> <p>Thus, <it>cadF </it>(-like) gene is highly conserved among <it>C. lari </it>organisms. Transcription of the <it>cadF </it>(-like) gene in <it>C. lari </it>cells <it>in vivo </it>was also confirmed and the transcription initiation site was determined. A peptidoglycan-associating alpha-helical motif in the C-terminal regions of some bacterial cell-surface proteins was completely conserved amongst the putative <it>cadF </it>(-like) ORFs from the <it>C. lari </it>isolates.</p> <p>Conclusion</p> <p>The putative <it>cadF </it>(-like) ORFs from all <it>C. lari </it>isolates were nine amino acid larger than those from <it>C. jejuni</it>, and showed amino acid residues 137 -140 of FALG (50% identity), instead of the FRLS residues of the maximal fibronectin-binding activity site demonstrated within <it>C. jejuni </it>CadF. A neighbor joining tree constructed based on <it>cadF </it>(-like) gene sequence information formed a major cluster consisting of <it>C. lari </it>isolates, separating from the other three thermophilic campylobacters.</p>
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