Structural analysis of the full-length gene encoding a fibronectin-binding-like protein (CadF) and its adjacent genetic loci within <it>Campylobacter lari</it>
<p>Abstract</p> <p>Background</p> <p>The combined sequences encoding a partial and putative <it>rpsI </it>open reading frame (ORF), non-coding (NC) region, a putative ORF for the <it>Campylobacter </it>adhesin to fibronectin-like protein (<it&...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-09-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/9/192 |
| _version_ | 1819053617559109632 |
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| author | Millar B Cherie Moore John E Taneike Ikue Tazumi Akihiro Sekizuka Tsuyoshi Hirayama Junichi Matsuda Motoo |
| author_facet | Millar B Cherie Moore John E Taneike Ikue Tazumi Akihiro Sekizuka Tsuyoshi Hirayama Junichi Matsuda Motoo |
| author_sort | Millar B Cherie |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The combined sequences encoding a partial and putative <it>rpsI </it>open reading frame (ORF), non-coding (NC) region, a putative ORF for the <it>Campylobacter </it>adhesin to fibronectin-like protein (<it>cadF</it>), a putative Cla_0387 ORF, NC region and a partial and putative Cla_0388 ORF, were identified in 16 <it>Campylobacter lari </it>isolates, using two novel degenerate primer pairs. Probable consensus sequence at the -35 and -10 regions were identified in all <it>C. lari </it>isolates, as a promoter.</p> <p>Results</p> <p>Thus, <it>cadF </it>(-like) gene is highly conserved among <it>C. lari </it>organisms. Transcription of the <it>cadF </it>(-like) gene in <it>C. lari </it>cells <it>in vivo </it>was also confirmed and the transcription initiation site was determined. A peptidoglycan-associating alpha-helical motif in the C-terminal regions of some bacterial cell-surface proteins was completely conserved amongst the putative <it>cadF </it>(-like) ORFs from the <it>C. lari </it>isolates.</p> <p>Conclusion</p> <p>The putative <it>cadF </it>(-like) ORFs from all <it>C. lari </it>isolates were nine amino acid larger than those from <it>C. jejuni</it>, and showed amino acid residues 137 -140 of FALG (50% identity), instead of the FRLS residues of the maximal fibronectin-binding activity site demonstrated within <it>C. jejuni </it>CadF. A neighbor joining tree constructed based on <it>cadF </it>(-like) gene sequence information formed a major cluster consisting of <it>C. lari </it>isolates, separating from the other three thermophilic campylobacters.</p> |
| first_indexed | 2024-12-21T12:38:35Z |
| format | Article |
| id | doaj.art-48c3d058fbd245f88ca7d7a332776b14 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-21T12:38:35Z |
| publishDate | 2009-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-48c3d058fbd245f88ca7d7a332776b142022-12-21T19:03:51ZengBMCBMC Microbiology1471-21802009-09-019119210.1186/1471-2180-9-192Structural analysis of the full-length gene encoding a fibronectin-binding-like protein (CadF) and its adjacent genetic loci within <it>Campylobacter lari</it>Millar B CherieMoore John ETaneike IkueTazumi AkihiroSekizuka TsuyoshiHirayama JunichiMatsuda Motoo<p>Abstract</p> <p>Background</p> <p>The combined sequences encoding a partial and putative <it>rpsI </it>open reading frame (ORF), non-coding (NC) region, a putative ORF for the <it>Campylobacter </it>adhesin to fibronectin-like protein (<it>cadF</it>), a putative Cla_0387 ORF, NC region and a partial and putative Cla_0388 ORF, were identified in 16 <it>Campylobacter lari </it>isolates, using two novel degenerate primer pairs. Probable consensus sequence at the -35 and -10 regions were identified in all <it>C. lari </it>isolates, as a promoter.</p> <p>Results</p> <p>Thus, <it>cadF </it>(-like) gene is highly conserved among <it>C. lari </it>organisms. Transcription of the <it>cadF </it>(-like) gene in <it>C. lari </it>cells <it>in vivo </it>was also confirmed and the transcription initiation site was determined. A peptidoglycan-associating alpha-helical motif in the C-terminal regions of some bacterial cell-surface proteins was completely conserved amongst the putative <it>cadF </it>(-like) ORFs from the <it>C. lari </it>isolates.</p> <p>Conclusion</p> <p>The putative <it>cadF </it>(-like) ORFs from all <it>C. lari </it>isolates were nine amino acid larger than those from <it>C. jejuni</it>, and showed amino acid residues 137 -140 of FALG (50% identity), instead of the FRLS residues of the maximal fibronectin-binding activity site demonstrated within <it>C. jejuni </it>CadF. A neighbor joining tree constructed based on <it>cadF </it>(-like) gene sequence information formed a major cluster consisting of <it>C. lari </it>isolates, separating from the other three thermophilic campylobacters.</p>http://www.biomedcentral.com/1471-2180/9/192 |
| spellingShingle | Millar B Cherie Moore John E Taneike Ikue Tazumi Akihiro Sekizuka Tsuyoshi Hirayama Junichi Matsuda Motoo Structural analysis of the full-length gene encoding a fibronectin-binding-like protein (CadF) and its adjacent genetic loci within <it>Campylobacter lari</it> BMC Microbiology |
| title | Structural analysis of the full-length gene encoding a fibronectin-binding-like protein (CadF) and its adjacent genetic loci within <it>Campylobacter lari</it> |
| title_full | Structural analysis of the full-length gene encoding a fibronectin-binding-like protein (CadF) and its adjacent genetic loci within <it>Campylobacter lari</it> |
| title_fullStr | Structural analysis of the full-length gene encoding a fibronectin-binding-like protein (CadF) and its adjacent genetic loci within <it>Campylobacter lari</it> |
| title_full_unstemmed | Structural analysis of the full-length gene encoding a fibronectin-binding-like protein (CadF) and its adjacent genetic loci within <it>Campylobacter lari</it> |
| title_short | Structural analysis of the full-length gene encoding a fibronectin-binding-like protein (CadF) and its adjacent genetic loci within <it>Campylobacter lari</it> |
| title_sort | structural analysis of the full length gene encoding a fibronectin binding like protein cadf and its adjacent genetic loci within it campylobacter lari it |
| url | http://www.biomedcentral.com/1471-2180/9/192 |
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