Protective effect of free phenolics from Lycopus lucidus Turcz. root on carbon tetrachloride-induced liver injury in vivo and in vitro
Protective effect of free phenolics from Lycopus lucidus Turcz. root (FPLR) on CCl4-induced hepatotoxicity in vivo and in vitro was first evaluated. Oral administration of FPLR (100 mg/kg bw) to mice significantly reduced the CCl4-induced elevation of serum alanine aminotransferase, aspartate aminot...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Swedish Nutrition Foundation
2018-07-01
|
| Series: | Food & Nutrition Research |
| Subjects: | |
| Online Access: | https://foodandnutritionresearch.net/index.php/fnr/article/view/1398/4835 |
| _version_ | 1819266658500345856 |
|---|---|
| author | Yue-Hong Lu Cheng-Rui Tian Chun-Yan Gao Wen-Jing Wang Wen-Yi Yang Xiao Kong You-Xia Chen Zhen-Zhen Liu |
| author_facet | Yue-Hong Lu Cheng-Rui Tian Chun-Yan Gao Wen-Jing Wang Wen-Yi Yang Xiao Kong You-Xia Chen Zhen-Zhen Liu |
| author_sort | Yue-Hong Lu |
| collection | DOAJ |
| description | Protective effect of free phenolics from Lycopus lucidus Turcz. root (FPLR) on CCl4-induced hepatotoxicity in vivo and in vitro was first evaluated. Oral administration of FPLR (100 mg/kg bw) to mice significantly reduced the CCl4-induced elevation of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, triacylglycerols, total cholesterol, and total bilirubin. FPLR also increased the hepatic GSH contents and antioxidant enzyme activities of SOD and CAT and decreased the hepatic MDA level. Histopathological examinations further confirmed that the FPLR could protect the liver from CCl4-induced damage. Further research indicated that FPLR prevented the DNA fragmentation caused by CCl4 based on TUNEL assay. Moreover, immunohistochemistry staining demonstrated that pretreatment with FPLR significantly inhibited the elevation of hepatic TNF-α, IL-6, IL-8, iNOS, COX-2, and Caspase-3 in CCl4-treated mice. In vitro experiments showed that FPLR remarkably reduced BRL hepatocyte apoptosis and damage caused by CCl4 treatment. These findings indicate that FPLR could be developed as a functional food or medication for therapeutic purpose and prevention of hepatic injury. |
| first_indexed | 2024-12-23T21:04:46Z |
| format | Article |
| id | doaj.art-48c4abc5405a44b5a538b64a20582626 |
| institution | Directory Open Access Journal |
| issn | 1654-661X |
| language | English |
| last_indexed | 2024-12-23T21:04:46Z |
| publishDate | 2018-07-01 |
| publisher | Swedish Nutrition Foundation |
| record_format | Article |
| series | Food & Nutrition Research |
| spelling | doaj.art-48c4abc5405a44b5a538b64a205826262022-12-21T17:31:16ZengSwedish Nutrition FoundationFood & Nutrition Research1654-661X2018-07-0162011010.29219/fnr.v62.13981398Protective effect of free phenolics from Lycopus lucidus Turcz. root on carbon tetrachloride-induced liver injury in vivo and in vitroYue-Hong Lu0Cheng-Rui Tian1Chun-Yan Gao2Wen-Jing Wang3Wen-Yi Yang4Xiao Kong5You-Xia Chen6Zhen-Zhen Liu7College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an, ChinaCollege of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an, ChinaSchool of Public Health, Dali University, Dali, ChinaSchool of Public Health, Dali University, Dali, ChinaSchool of Public Health, Dali University, Dali, ChinaSchool of Public Health, Dali University, Dali, ChinaSchool of Public Health, Dali University, Dali, ChinaSchool of Public Health, Dali University, Dali, ChinaProtective effect of free phenolics from Lycopus lucidus Turcz. root (FPLR) on CCl4-induced hepatotoxicity in vivo and in vitro was first evaluated. Oral administration of FPLR (100 mg/kg bw) to mice significantly reduced the CCl4-induced elevation of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, triacylglycerols, total cholesterol, and total bilirubin. FPLR also increased the hepatic GSH contents and antioxidant enzyme activities of SOD and CAT and decreased the hepatic MDA level. Histopathological examinations further confirmed that the FPLR could protect the liver from CCl4-induced damage. Further research indicated that FPLR prevented the DNA fragmentation caused by CCl4 based on TUNEL assay. Moreover, immunohistochemistry staining demonstrated that pretreatment with FPLR significantly inhibited the elevation of hepatic TNF-α, IL-6, IL-8, iNOS, COX-2, and Caspase-3 in CCl4-treated mice. In vitro experiments showed that FPLR remarkably reduced BRL hepatocyte apoptosis and damage caused by CCl4 treatment. These findings indicate that FPLR could be developed as a functional food or medication for therapeutic purpose and prevention of hepatic injury.https://foodandnutritionresearch.net/index.php/fnr/article/view/1398/4835Lycopus lucidus Turcz.Free phenolicsCarbon tetrachlorideHepatoprotective effect |
| spellingShingle | Yue-Hong Lu Cheng-Rui Tian Chun-Yan Gao Wen-Jing Wang Wen-Yi Yang Xiao Kong You-Xia Chen Zhen-Zhen Liu Protective effect of free phenolics from Lycopus lucidus Turcz. root on carbon tetrachloride-induced liver injury in vivo and in vitro Food & Nutrition Research Lycopus lucidus Turcz. Free phenolics Carbon tetrachloride Hepatoprotective effect |
| title | Protective effect of free phenolics from Lycopus lucidus Turcz. root on carbon tetrachloride-induced liver injury in vivo and in vitro |
| title_full | Protective effect of free phenolics from Lycopus lucidus Turcz. root on carbon tetrachloride-induced liver injury in vivo and in vitro |
| title_fullStr | Protective effect of free phenolics from Lycopus lucidus Turcz. root on carbon tetrachloride-induced liver injury in vivo and in vitro |
| title_full_unstemmed | Protective effect of free phenolics from Lycopus lucidus Turcz. root on carbon tetrachloride-induced liver injury in vivo and in vitro |
| title_short | Protective effect of free phenolics from Lycopus lucidus Turcz. root on carbon tetrachloride-induced liver injury in vivo and in vitro |
| title_sort | protective effect of free phenolics from lycopus lucidus turcz root on carbon tetrachloride induced liver injury in vivo and in vitro |
| topic | Lycopus lucidus Turcz. Free phenolics Carbon tetrachloride Hepatoprotective effect |
| url | https://foodandnutritionresearch.net/index.php/fnr/article/view/1398/4835 |
| work_keys_str_mv | AT yuehonglu protectiveeffectoffreephenolicsfromlycopuslucidusturczrootoncarbontetrachlorideinducedliverinjuryinvivoandinvitro AT chengruitian protectiveeffectoffreephenolicsfromlycopuslucidusturczrootoncarbontetrachlorideinducedliverinjuryinvivoandinvitro AT chunyangao protectiveeffectoffreephenolicsfromlycopuslucidusturczrootoncarbontetrachlorideinducedliverinjuryinvivoandinvitro AT wenjingwang protectiveeffectoffreephenolicsfromlycopuslucidusturczrootoncarbontetrachlorideinducedliverinjuryinvivoandinvitro AT wenyiyang protectiveeffectoffreephenolicsfromlycopuslucidusturczrootoncarbontetrachlorideinducedliverinjuryinvivoandinvitro AT xiaokong protectiveeffectoffreephenolicsfromlycopuslucidusturczrootoncarbontetrachlorideinducedliverinjuryinvivoandinvitro AT youxiachen protectiveeffectoffreephenolicsfromlycopuslucidusturczrootoncarbontetrachlorideinducedliverinjuryinvivoandinvitro AT zhenzhenliu protectiveeffectoffreephenolicsfromlycopuslucidusturczrootoncarbontetrachlorideinducedliverinjuryinvivoandinvitro |