Leismanicidal Activity of Propolis Collected in the Semiarid Region of Brazil
Objective: The aim of the current study is to investigate the chemical composition, cytotoxic effect, and leishmanicidal activity of propolis collected in the semi-arid region of Bahia, Brazil.Methods: EtOH extract, hexane, EtOAc and MeOH fractions from propolis were analyzed by ultra-performance li...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.702032/full |
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| author | Giani Maria Cavalcante Celso Amorim Camara Eva Monica Sarmento Da Silva Mariana Silva Santos Anderson Brandão Leite Aline Cavalcanti Queiroz Aline Cavalcanti Queiroz Amanda Evelyn Da Silva Morgana Vital Araújo Magna Suzana Alexandre-Moreira Tania Maria Sarmento Silva |
| author_facet | Giani Maria Cavalcante Celso Amorim Camara Eva Monica Sarmento Da Silva Mariana Silva Santos Anderson Brandão Leite Aline Cavalcanti Queiroz Aline Cavalcanti Queiroz Amanda Evelyn Da Silva Morgana Vital Araújo Magna Suzana Alexandre-Moreira Tania Maria Sarmento Silva |
| author_sort | Giani Maria Cavalcante |
| collection | DOAJ |
| description | Objective: The aim of the current study is to investigate the chemical composition, cytotoxic effect, and leishmanicidal activity of propolis collected in the semi-arid region of Bahia, Brazil.Methods: EtOH extract, hexane, EtOAc and MeOH fractions from propolis were analyzed by ultra-performance liquid chromatography coupled with diode array detector and quadrupole time-of-flight mass spectrometry. The identification was based on the exact mass, general fragmentation behaviors and UV absorption of the flavonoids. The in vitro cytotoxic effect and leishmanicidal activity of ethanolic extract, hexane, ethyl acetate, and methanolic fractions of propolis were evaluated.Results: Five triterpenes and twenty-four flavonoids were identified. The propolis did not present toxicity to the host cell up to the maximum concentration tested. In addition, all tested samples showed statistically significant activity against promastigotes of Leishmania chagasi and Leishmania amazonensis. Regarding the activity against amastigote forms of L. amazonensis, the hexane fraction, presented statistically significant activity with IC50 of 1.3 ± 0.1 μg/ml.Conclusion: The results support the idea that propolis can be used for future antileishmania studies. |
| first_indexed | 2024-12-19T06:13:29Z |
| format | Article |
| id | doaj.art-48cb3c2494f54cd08cbc0d40fe17fea1 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-19T06:13:29Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-48cb3c2494f54cd08cbc0d40fe17fea12022-12-21T20:32:56ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-07-011210.3389/fphar.2021.702032702032Leismanicidal Activity of Propolis Collected in the Semiarid Region of BrazilGiani Maria Cavalcante0Celso Amorim Camara1Eva Monica Sarmento Da Silva2Mariana Silva Santos3Anderson Brandão Leite4Aline Cavalcanti Queiroz5Aline Cavalcanti Queiroz6Amanda Evelyn Da Silva7Morgana Vital Araújo8Magna Suzana Alexandre-Moreira9Tania Maria Sarmento Silva10Phytochemical Bioprospecting Laboratory, Chemistry Department, Rural Federal University of Pernambuco, Pernambuco, BrazilPhytochemical Bioprospecting Laboratory, Chemistry Department, Rural Federal University of Pernambuco, Pernambuco, BrazilZootechnical Collegiate, Federal University of the São Francisco Valley, Pernambuco, BrazilPharmacology and Immunity Laboratory, Institute of Biological and Health Sciences, Federal University of Alagoas, Alagoas, BrazilPharmacology and Immunity Laboratory, Institute of Biological and Health Sciences, Federal University of Alagoas, Alagoas, BrazilPharmacology and Immunity Laboratory, Institute of Biological and Health Sciences, Federal University of Alagoas, Alagoas, BrazilMicrobiology, Immunology and Parasitology Laboratory, Campus Arapiraca, Federal University of Alagoas, Alagoas, BrazilPharmacology and Immunity Laboratory, Institute of Biological and Health Sciences, Federal University of Alagoas, Alagoas, BrazilPharmacology and Immunity Laboratory, Institute of Biological and Health Sciences, Federal University of Alagoas, Alagoas, BrazilPharmacology and Immunity Laboratory, Institute of Biological and Health Sciences, Federal University of Alagoas, Alagoas, BrazilPhytochemical Bioprospecting Laboratory, Chemistry Department, Rural Federal University of Pernambuco, Pernambuco, BrazilObjective: The aim of the current study is to investigate the chemical composition, cytotoxic effect, and leishmanicidal activity of propolis collected in the semi-arid region of Bahia, Brazil.Methods: EtOH extract, hexane, EtOAc and MeOH fractions from propolis were analyzed by ultra-performance liquid chromatography coupled with diode array detector and quadrupole time-of-flight mass spectrometry. The identification was based on the exact mass, general fragmentation behaviors and UV absorption of the flavonoids. The in vitro cytotoxic effect and leishmanicidal activity of ethanolic extract, hexane, ethyl acetate, and methanolic fractions of propolis were evaluated.Results: Five triterpenes and twenty-four flavonoids were identified. The propolis did not present toxicity to the host cell up to the maximum concentration tested. In addition, all tested samples showed statistically significant activity against promastigotes of Leishmania chagasi and Leishmania amazonensis. Regarding the activity against amastigote forms of L. amazonensis, the hexane fraction, presented statistically significant activity with IC50 of 1.3 ± 0.1 μg/ml.Conclusion: The results support the idea that propolis can be used for future antileishmania studies.https://www.frontiersin.org/articles/10.3389/fphar.2021.702032/fullflavonoidspropolisleishmanicidal activityLeishmania (Leishmania) amazonensisLeishmania (Leishmania) chagasi |
| spellingShingle | Giani Maria Cavalcante Celso Amorim Camara Eva Monica Sarmento Da Silva Mariana Silva Santos Anderson Brandão Leite Aline Cavalcanti Queiroz Aline Cavalcanti Queiroz Amanda Evelyn Da Silva Morgana Vital Araújo Magna Suzana Alexandre-Moreira Tania Maria Sarmento Silva Leismanicidal Activity of Propolis Collected in the Semiarid Region of Brazil Frontiers in Pharmacology flavonoids propolis leishmanicidal activity Leishmania (Leishmania) amazonensis Leishmania (Leishmania) chagasi |
| title | Leismanicidal Activity of Propolis Collected in the Semiarid Region of Brazil |
| title_full | Leismanicidal Activity of Propolis Collected in the Semiarid Region of Brazil |
| title_fullStr | Leismanicidal Activity of Propolis Collected in the Semiarid Region of Brazil |
| title_full_unstemmed | Leismanicidal Activity of Propolis Collected in the Semiarid Region of Brazil |
| title_short | Leismanicidal Activity of Propolis Collected in the Semiarid Region of Brazil |
| title_sort | leismanicidal activity of propolis collected in the semiarid region of brazil |
| topic | flavonoids propolis leishmanicidal activity Leishmania (Leishmania) amazonensis Leishmania (Leishmania) chagasi |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2021.702032/full |
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