Linkage mapping and quantitative trait loci analysis of sweetness and other fruit quality traits in papaya
Abstract Background The identification and characterisation of quantitative trait loci (QTL) is an important step towards identifying functional sequences underpinning important crop traits and for developing accurate markers for selective breeding strategies. In this study, a genotyping-by-sequenci...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-019-2043-0 |
| _version_ | 1819006870777495552 |
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| author | Usana Nantawan Chutchamas Kanchana-udomkan Ido Bar Rebecca Ford |
| author_facet | Usana Nantawan Chutchamas Kanchana-udomkan Ido Bar Rebecca Ford |
| author_sort | Usana Nantawan |
| collection | DOAJ |
| description | Abstract Background The identification and characterisation of quantitative trait loci (QTL) is an important step towards identifying functional sequences underpinning important crop traits and for developing accurate markers for selective breeding strategies. In this study, a genotyping-by-sequencing (GBS) approach detected QTL conditioning desirable fruit quality traits in papaya. Results For this, a linkage map was constructed comprising 219 single nucleotide polymorphism (SNP) loci across 10 linkage groups and covering 509 centiMorgan (cM). In total, 21 QTLs were identified for seven key fruit quality traits, including flesh sweetness, fruit weight, fruit length, fruit width skin freckle, flesh thickness and fruit firmness. Several QTL for flesh sweetness, fruit weight, length, width and firmness were stable across harvest years and individually explained up to 19.8% of the phenotypic variance of a particular trait. Where possible, candidate genes were proposed and explored further for their application to marker-assisted breeding. Conclusions This study has extended knowledge on the inheritance and genetic control for key papaya physiological and fruit quality traits. Candidate genes together with associated SNP markers represent a valuable resource for the future of strategic selective breeding of elite Australian papaya cultivars. |
| first_indexed | 2024-12-21T00:15:34Z |
| format | Article |
| id | doaj.art-48d14501632443299dc15eb64f63c7f4 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-21T00:15:34Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-48d14501632443299dc15eb64f63c7f42022-12-21T19:22:15ZengBMCBMC Plant Biology1471-22292019-10-0119111110.1186/s12870-019-2043-0Linkage mapping and quantitative trait loci analysis of sweetness and other fruit quality traits in papayaUsana Nantawan0Chutchamas Kanchana-udomkan1Ido Bar2Rebecca Ford3Environmental Futures Research Institute, School of Environment and Sciences, Griffith UniversityEnvironmental Futures Research Institute, School of Environment and Sciences, Griffith UniversityEnvironmental Futures Research Institute, School of Environment and Sciences, Griffith UniversityEnvironmental Futures Research Institute, School of Environment and Sciences, Griffith UniversityAbstract Background The identification and characterisation of quantitative trait loci (QTL) is an important step towards identifying functional sequences underpinning important crop traits and for developing accurate markers for selective breeding strategies. In this study, a genotyping-by-sequencing (GBS) approach detected QTL conditioning desirable fruit quality traits in papaya. Results For this, a linkage map was constructed comprising 219 single nucleotide polymorphism (SNP) loci across 10 linkage groups and covering 509 centiMorgan (cM). In total, 21 QTLs were identified for seven key fruit quality traits, including flesh sweetness, fruit weight, fruit length, fruit width skin freckle, flesh thickness and fruit firmness. Several QTL for flesh sweetness, fruit weight, length, width and firmness were stable across harvest years and individually explained up to 19.8% of the phenotypic variance of a particular trait. Where possible, candidate genes were proposed and explored further for their application to marker-assisted breeding. Conclusions This study has extended knowledge on the inheritance and genetic control for key papaya physiological and fruit quality traits. Candidate genes together with associated SNP markers represent a valuable resource for the future of strategic selective breeding of elite Australian papaya cultivars.http://link.springer.com/article/10.1186/s12870-019-2043-0Papaya breedingFruit quality traitGBSQTL analysisSNP markers |
| spellingShingle | Usana Nantawan Chutchamas Kanchana-udomkan Ido Bar Rebecca Ford Linkage mapping and quantitative trait loci analysis of sweetness and other fruit quality traits in papaya BMC Plant Biology Papaya breeding Fruit quality trait GBS QTL analysis SNP markers |
| title | Linkage mapping and quantitative trait loci analysis of sweetness and other fruit quality traits in papaya |
| title_full | Linkage mapping and quantitative trait loci analysis of sweetness and other fruit quality traits in papaya |
| title_fullStr | Linkage mapping and quantitative trait loci analysis of sweetness and other fruit quality traits in papaya |
| title_full_unstemmed | Linkage mapping and quantitative trait loci analysis of sweetness and other fruit quality traits in papaya |
| title_short | Linkage mapping and quantitative trait loci analysis of sweetness and other fruit quality traits in papaya |
| title_sort | linkage mapping and quantitative trait loci analysis of sweetness and other fruit quality traits in papaya |
| topic | Papaya breeding Fruit quality trait GBS QTL analysis SNP markers |
| url | http://link.springer.com/article/10.1186/s12870-019-2043-0 |
| work_keys_str_mv | AT usananantawan linkagemappingandquantitativetraitlocianalysisofsweetnessandotherfruitqualitytraitsinpapaya AT chutchamaskanchanaudomkan linkagemappingandquantitativetraitlocianalysisofsweetnessandotherfruitqualitytraitsinpapaya AT idobar linkagemappingandquantitativetraitlocianalysisofsweetnessandotherfruitqualitytraitsinpapaya AT rebeccaford linkagemappingandquantitativetraitlocianalysisofsweetnessandotherfruitqualitytraitsinpapaya |