DNA-PAINT super-resolution imaging data of surface exposed active sites on particles
Surface functionalization with targeting ligands confers to nanomaterials the ability of selectively recognize a biological target. Therefore, a quantitative characterization of surface functional molecules is critical for the rational development of nanomaterials-based applications, especially in n...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340920303620 |
| _version_ | 1819266037182365696 |
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| author | Pietro Delcanale Lorenzo Albertazzi |
| author_facet | Pietro Delcanale Lorenzo Albertazzi |
| author_sort | Pietro Delcanale |
| collection | DOAJ |
| description | Surface functionalization with targeting ligands confers to nanomaterials the ability of selectively recognize a biological target. Therefore, a quantitative characterization of surface functional molecules is critical for the rational development of nanomaterials-based applications, especially in nanomedicine research. Single-molecule localization microscopy can provide visualization of surface molecules at the level of individual particles, preserving the integrity of the material and overcoming the limitations of analytical methods based on ensemble averaging. Here we provide single-molecule localization data obtained on streptavidin-coated polystyrene particles, which can be exploited as a model system for surface-functionalized materials. After loading of the active sites of streptavidin molecules with a biotin-conjugated probe, they were imaged with a DNA-PAINT imaging approach, which can provide single-molecule imaging at subdiffraction resolution and molecule counting. Both raw records and analysed data, consisting in a list of space-time single-molecule coordinates, are shared. Additionally, Matlab functions are provided that analyse the single-molecule coordinates in order to quantify features of individual particles. These data might constitute a valuable reference for applications of similar quantitative imaging methodologies to other types of functionalized nanomaterials. |
| first_indexed | 2024-12-23T20:54:54Z |
| format | Article |
| id | doaj.art-48d94f291ddf4f11bcc65bdf448226e2 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-23T20:54:54Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-48d94f291ddf4f11bcc65bdf448226e22022-12-21T17:31:33ZengElsevierData in Brief2352-34092020-06-0130105468DNA-PAINT super-resolution imaging data of surface exposed active sites on particlesPietro Delcanale0Lorenzo Albertazzi1Instutite for Bioengineering of Catalonia (IBEC), The Barcelona institute of Science and Technology (BIST), Baldiri Reixac 15-21, 08028, Barcelona, SpainInstutite for Bioengineering of Catalonia (IBEC), The Barcelona institute of Science and Technology (BIST), Baldiri Reixac 15-21, 08028, Barcelona, Spain; Department of Biomedical Engineering and Institute of Complex Molecular Systems (ICMS), Eindhoven University of Technology, 5612AZ Eindhoven, the Netherlands; Corresponding author at: Department of Biomedical Engineering and Institute of Complex Molecular Systems (ICMS), Eindhoven University of Technology, 5612AZ Eindhoven, the Netherlands.Surface functionalization with targeting ligands confers to nanomaterials the ability of selectively recognize a biological target. Therefore, a quantitative characterization of surface functional molecules is critical for the rational development of nanomaterials-based applications, especially in nanomedicine research. Single-molecule localization microscopy can provide visualization of surface molecules at the level of individual particles, preserving the integrity of the material and overcoming the limitations of analytical methods based on ensemble averaging. Here we provide single-molecule localization data obtained on streptavidin-coated polystyrene particles, which can be exploited as a model system for surface-functionalized materials. After loading of the active sites of streptavidin molecules with a biotin-conjugated probe, they were imaged with a DNA-PAINT imaging approach, which can provide single-molecule imaging at subdiffraction resolution and molecule counting. Both raw records and analysed data, consisting in a list of space-time single-molecule coordinates, are shared. Additionally, Matlab functions are provided that analyse the single-molecule coordinates in order to quantify features of individual particles. These data might constitute a valuable reference for applications of similar quantitative imaging methodologies to other types of functionalized nanomaterials.http://www.sciencedirect.com/science/article/pii/S2352340920303620Single-molecule localization microscopySuper-resolution microscopyDNA-PAINTNanoparticlesFunctional materials |
| spellingShingle | Pietro Delcanale Lorenzo Albertazzi DNA-PAINT super-resolution imaging data of surface exposed active sites on particles Data in Brief Single-molecule localization microscopy Super-resolution microscopy DNA-PAINT Nanoparticles Functional materials |
| title | DNA-PAINT super-resolution imaging data of surface exposed active sites on particles |
| title_full | DNA-PAINT super-resolution imaging data of surface exposed active sites on particles |
| title_fullStr | DNA-PAINT super-resolution imaging data of surface exposed active sites on particles |
| title_full_unstemmed | DNA-PAINT super-resolution imaging data of surface exposed active sites on particles |
| title_short | DNA-PAINT super-resolution imaging data of surface exposed active sites on particles |
| title_sort | dna paint super resolution imaging data of surface exposed active sites on particles |
| topic | Single-molecule localization microscopy Super-resolution microscopy DNA-PAINT Nanoparticles Functional materials |
| url | http://www.sciencedirect.com/science/article/pii/S2352340920303620 |
| work_keys_str_mv | AT pietrodelcanale dnapaintsuperresolutionimagingdataofsurfaceexposedactivesitesonparticles AT lorenzoalbertazzi dnapaintsuperresolutionimagingdataofsurfaceexposedactivesitesonparticles |