Identification of QTLs controlling grain protein concentration using a high-density SNP and SSR linkage map in barley (Hordeum vulgare L.)
Abstract Background Grain protein concentration (GPC) is a major determinant of quality in barley (Hordeum vulgare L.). Breeding barley cultivars with high GPC has practical value for feed and food properties. The aim of the present study was to identify quantitative trait loci (QTLs) for GPC that c...
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| Format: | Article |
| Language: | English |
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BMC
2017-07-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-017-1067-6 |
| _version_ | 1818902636102942720 |
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| author | Chaofeng Fan Huijie Zhai Huifang Wang Yafei Yue Minghu Zhang Jinghui Li Shaozhe Wen Ganggang Guo Yawen Zeng Zhongfu Ni Mingshan You |
| author_facet | Chaofeng Fan Huijie Zhai Huifang Wang Yafei Yue Minghu Zhang Jinghui Li Shaozhe Wen Ganggang Guo Yawen Zeng Zhongfu Ni Mingshan You |
| author_sort | Chaofeng Fan |
| collection | DOAJ |
| description | Abstract Background Grain protein concentration (GPC) is a major determinant of quality in barley (Hordeum vulgare L.). Breeding barley cultivars with high GPC has practical value for feed and food properties. The aim of the present study was to identify quantitative trait loci (QTLs) for GPC that could be detected under multiple environments. Results A population of 190 recombinant inbred lines (RILs) deriving from a cross between Chinese landrace ZGMLEL with high GPC (> 20%) and Australian cultivar Schooner was used for linkage and QTL analyses. The genetic linkage map spanned 2353.48 cM in length with an average locus interval of 2.33 cM. GPC was evaluated under six environments for the RIL population and the two parental lines. In total, six environmentally stable QTLs for GPC were detected on chromosomes 2H (1), 4H (1), 6H (1), and 7H (3) and the increasing alleles were derived from ZGMLEL. Notably, the three QTLs on chromosome 7H (QGpc.ZiSc-7H.1, QGpc.ZiSc-7H.2, and QGpc.ZiSc-7H.3) that linked in coupling phase were firstly identified. Moreover, the genetic effects of stable QTLs on chromosomes 2H, 6H and 7H were validated using near isogenic lines (NILs). Conclusions Collectively, the identified QTLs expanded our knowledge about the genetic basis of GPC in barley and could be selected to develop cultivars with high grain protein concentration. |
| first_indexed | 2024-12-19T20:38:48Z |
| format | Article |
| id | doaj.art-48dbb4cc3de747d0a5e288f9ee41fe66 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-19T20:38:48Z |
| publishDate | 2017-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-48dbb4cc3de747d0a5e288f9ee41fe662022-12-21T20:06:28ZengBMCBMC Plant Biology1471-22292017-07-0117111410.1186/s12870-017-1067-6Identification of QTLs controlling grain protein concentration using a high-density SNP and SSR linkage map in barley (Hordeum vulgare L.)Chaofeng Fan0Huijie Zhai1Huifang Wang2Yafei Yue3Minghu Zhang4Jinghui Li5Shaozhe Wen6Ganggang Guo7Yawen Zeng8Zhongfu Ni9Mingshan You10State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural UniversityState Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural UniversityState Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural UniversityState Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural UniversityState Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural UniversityState Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural UniversityState Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural UniversityInstitute of Crop Science, Chinese Academy of Agriculture SciencesBiotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural SciencesState Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural UniversityState Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural UniversityAbstract Background Grain protein concentration (GPC) is a major determinant of quality in barley (Hordeum vulgare L.). Breeding barley cultivars with high GPC has practical value for feed and food properties. The aim of the present study was to identify quantitative trait loci (QTLs) for GPC that could be detected under multiple environments. Results A population of 190 recombinant inbred lines (RILs) deriving from a cross between Chinese landrace ZGMLEL with high GPC (> 20%) and Australian cultivar Schooner was used for linkage and QTL analyses. The genetic linkage map spanned 2353.48 cM in length with an average locus interval of 2.33 cM. GPC was evaluated under six environments for the RIL population and the two parental lines. In total, six environmentally stable QTLs for GPC were detected on chromosomes 2H (1), 4H (1), 6H (1), and 7H (3) and the increasing alleles were derived from ZGMLEL. Notably, the three QTLs on chromosome 7H (QGpc.ZiSc-7H.1, QGpc.ZiSc-7H.2, and QGpc.ZiSc-7H.3) that linked in coupling phase were firstly identified. Moreover, the genetic effects of stable QTLs on chromosomes 2H, 6H and 7H were validated using near isogenic lines (NILs). Conclusions Collectively, the identified QTLs expanded our knowledge about the genetic basis of GPC in barley and could be selected to develop cultivars with high grain protein concentration.http://link.springer.com/article/10.1186/s12870-017-1067-6Barley (Hordeum vulgare L.)Grain protein concentration (GPC)QTLSNP markerNear isogenic line |
| spellingShingle | Chaofeng Fan Huijie Zhai Huifang Wang Yafei Yue Minghu Zhang Jinghui Li Shaozhe Wen Ganggang Guo Yawen Zeng Zhongfu Ni Mingshan You Identification of QTLs controlling grain protein concentration using a high-density SNP and SSR linkage map in barley (Hordeum vulgare L.) BMC Plant Biology Barley (Hordeum vulgare L.) Grain protein concentration (GPC) QTL SNP marker Near isogenic line |
| title | Identification of QTLs controlling grain protein concentration using a high-density SNP and SSR linkage map in barley (Hordeum vulgare L.) |
| title_full | Identification of QTLs controlling grain protein concentration using a high-density SNP and SSR linkage map in barley (Hordeum vulgare L.) |
| title_fullStr | Identification of QTLs controlling grain protein concentration using a high-density SNP and SSR linkage map in barley (Hordeum vulgare L.) |
| title_full_unstemmed | Identification of QTLs controlling grain protein concentration using a high-density SNP and SSR linkage map in barley (Hordeum vulgare L.) |
| title_short | Identification of QTLs controlling grain protein concentration using a high-density SNP and SSR linkage map in barley (Hordeum vulgare L.) |
| title_sort | identification of qtls controlling grain protein concentration using a high density snp and ssr linkage map in barley hordeum vulgare l |
| topic | Barley (Hordeum vulgare L.) Grain protein concentration (GPC) QTL SNP marker Near isogenic line |
| url | http://link.springer.com/article/10.1186/s12870-017-1067-6 |
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