Tailoring the formation and stability of self-assembled structures from precisely engineered intrinsically disordered protein polymers: A comprehensive review
Intrinsically disordered proteins (IDPs) are a class of proteins that lack stable three-dimensional structures. Despite their natural tendency to be disordered, precise modulations of molecular parameters (e.g., sequence, length) through biomolecular engineering tools and control of environmental co...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Giant |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666542523000206 |
| _version_ | 1797801700775428096 |
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| author | Fathima T. Doole Christopher P. Camp Minkyu Kim |
| author_facet | Fathima T. Doole Christopher P. Camp Minkyu Kim |
| author_sort | Fathima T. Doole |
| collection | DOAJ |
| description | Intrinsically disordered proteins (IDPs) are a class of proteins that lack stable three-dimensional structures. Despite their natural tendency to be disordered, precise modulations of molecular parameters (e.g., sequence, length) through biomolecular engineering tools and control of environmental conditions tailor the formation of dynamic self-assembled structures. In addition to designing structures that respond to external stimuli for specific biotechnological applications (e.g., biosensors), other applications require stable structures (e.g., engineered tissues, drug delivery vehicles) that resist unintended changes and disassembly across various environmental conditions, such as different concentrations and temperatures. This review provides a comprehensive understanding of the design and engineering principles that govern the self-assembly of biosynthetic IDPs and their stability. Specifically, elastin-like polypeptides (ELPs) are highlighted as a prominent example of biosynthetically designed, thermoresponsive IDPs. Examples include ELPs that form various self-assembled structures by themselves as ELP homopolymers or diblock copolymers, ELPs combined with other IDPs in diblock copolymers, and ELP-based polymer hybrids containing functional (bio)molecules. It is anticipated that the efforts to enhance the stability of self-assembled structures through the precise engineering of IDP-based polymers have expanded the potential for diverse biotechnological applications in tissue engineering, drug delivery, diagnostic assays, and biomedicine. |
| first_indexed | 2024-03-13T04:54:28Z |
| format | Article |
| id | doaj.art-48dbe47f249642de8cd60370f5140beb |
| institution | Directory Open Access Journal |
| issn | 2666-5425 |
| language | English |
| last_indexed | 2024-03-13T04:54:28Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Giant |
| spelling | doaj.art-48dbe47f249642de8cd60370f5140beb2023-06-18T05:03:28ZengElsevierGiant2666-54252023-06-0114100158Tailoring the formation and stability of self-assembled structures from precisely engineered intrinsically disordered protein polymers: A comprehensive reviewFathima T. Doole0Christopher P. Camp1Minkyu Kim2Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721, USADepartment of Materials Science and Engineering, Department of Biomedical Engineering, University of Arizona, Tucson, AZ 85721, USADepartment of Materials Science and Engineering, Department of Biomedical Engineering, University of Arizona, Tucson, AZ 85721, USA; BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA; Corresponding author.Intrinsically disordered proteins (IDPs) are a class of proteins that lack stable three-dimensional structures. Despite their natural tendency to be disordered, precise modulations of molecular parameters (e.g., sequence, length) through biomolecular engineering tools and control of environmental conditions tailor the formation of dynamic self-assembled structures. In addition to designing structures that respond to external stimuli for specific biotechnological applications (e.g., biosensors), other applications require stable structures (e.g., engineered tissues, drug delivery vehicles) that resist unintended changes and disassembly across various environmental conditions, such as different concentrations and temperatures. This review provides a comprehensive understanding of the design and engineering principles that govern the self-assembly of biosynthetic IDPs and their stability. Specifically, elastin-like polypeptides (ELPs) are highlighted as a prominent example of biosynthetically designed, thermoresponsive IDPs. Examples include ELPs that form various self-assembled structures by themselves as ELP homopolymers or diblock copolymers, ELPs combined with other IDPs in diblock copolymers, and ELP-based polymer hybrids containing functional (bio)molecules. It is anticipated that the efforts to enhance the stability of self-assembled structures through the precise engineering of IDP-based polymers have expanded the potential for diverse biotechnological applications in tissue engineering, drug delivery, diagnostic assays, and biomedicine.http://www.sciencedirect.com/science/article/pii/S2666542523000206Intrinsically disordered proteinProtein polymerSelf-assemblyStimulus responsivenessStructural stability |
| spellingShingle | Fathima T. Doole Christopher P. Camp Minkyu Kim Tailoring the formation and stability of self-assembled structures from precisely engineered intrinsically disordered protein polymers: A comprehensive review Giant Intrinsically disordered protein Protein polymer Self-assembly Stimulus responsiveness Structural stability |
| title | Tailoring the formation and stability of self-assembled structures from precisely engineered intrinsically disordered protein polymers: A comprehensive review |
| title_full | Tailoring the formation and stability of self-assembled structures from precisely engineered intrinsically disordered protein polymers: A comprehensive review |
| title_fullStr | Tailoring the formation and stability of self-assembled structures from precisely engineered intrinsically disordered protein polymers: A comprehensive review |
| title_full_unstemmed | Tailoring the formation and stability of self-assembled structures from precisely engineered intrinsically disordered protein polymers: A comprehensive review |
| title_short | Tailoring the formation and stability of self-assembled structures from precisely engineered intrinsically disordered protein polymers: A comprehensive review |
| title_sort | tailoring the formation and stability of self assembled structures from precisely engineered intrinsically disordered protein polymers a comprehensive review |
| topic | Intrinsically disordered protein Protein polymer Self-assembly Stimulus responsiveness Structural stability |
| url | http://www.sciencedirect.com/science/article/pii/S2666542523000206 |
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