Characteristics and Potentiality of Human Adipose-Derived Stem Cells (hASCs) Obtained from Enzymatic Digestion of Fat Graft
Human adipose-derived stem cells localize in the stromal-vascular portion, and can be ex vivo isolated using a combination of washing steps and enzymatic digestion. For this study, we undertook a histological evaluation of traditional fat graft compared with fat graft enriched with stromal vascular...
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MDPI AG
2019-03-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/3/282 |
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| author | Pietro Gentile Maria Serena Piccinno Claudio Calabrese |
| author_facet | Pietro Gentile Maria Serena Piccinno Claudio Calabrese |
| author_sort | Pietro Gentile |
| collection | DOAJ |
| description | Human adipose-derived stem cells localize in the stromal-vascular portion, and can be ex vivo isolated using a combination of washing steps and enzymatic digestion. For this study, we undertook a histological evaluation of traditional fat graft compared with fat graft enriched with stromal vascular fraction cells isolated by the Celution™ system to assess the interactions between cells and adipose tissue before the breast injection. In addition, we reported on histological analyses of biopsies derived from fat grafted (traditional or enriched with SVFs) in the breast in order to assess the quality of the adipose tissue, fibrosis and vessels. The hASCs derived from enzymatic digestion were systematically characterized for growth features, phenotype and multi-potent differentiation potential. They fulfill the definition of mesenchymal stem cells, albeit with a higher neural phenotype profile. These cells also express genes that constitute the core circuitry of self-renewal such as OCT4, SOX2, NANOG and neurogenic lineage genes such as NEUROD1, PAX6 and SOX3. Such findings support the hypothesis that hASCs may have a potential usefulness in neurodegenerative conditions. These data can be helpful for the development of new therapeutic approaches in personalized medicine to assess safety and efficacy of the breast reconstruction. |
| first_indexed | 2024-03-12T08:14:45Z |
| format | Article |
| id | doaj.art-48e66846dc0448c0bd486aebb2985154 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T08:14:45Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj.art-48e66846dc0448c0bd486aebb29851542023-09-02T18:57:21ZengMDPI AGCells2073-44092019-03-018328210.3390/cells8030282cells8030282Characteristics and Potentiality of Human Adipose-Derived Stem Cells (hASCs) Obtained from Enzymatic Digestion of Fat GraftPietro Gentile0Maria Serena Piccinno1Claudio Calabrese2Department of Surgical Science, University of Rome Tor Vergata, Rome 00133, ItalyScientific and Technological Park fo Medicine “Mario Veronesi”, via 29 Maggio, 6, 41037 Mirandola, ItalyThe Oncologic and Reconstructive Surgery Breast Unit, Oncology Department, Careggi University Hospital, Firenze 50134, ItalyHuman adipose-derived stem cells localize in the stromal-vascular portion, and can be ex vivo isolated using a combination of washing steps and enzymatic digestion. For this study, we undertook a histological evaluation of traditional fat graft compared with fat graft enriched with stromal vascular fraction cells isolated by the Celution™ system to assess the interactions between cells and adipose tissue before the breast injection. In addition, we reported on histological analyses of biopsies derived from fat grafted (traditional or enriched with SVFs) in the breast in order to assess the quality of the adipose tissue, fibrosis and vessels. The hASCs derived from enzymatic digestion were systematically characterized for growth features, phenotype and multi-potent differentiation potential. They fulfill the definition of mesenchymal stem cells, albeit with a higher neural phenotype profile. These cells also express genes that constitute the core circuitry of self-renewal such as OCT4, SOX2, NANOG and neurogenic lineage genes such as NEUROD1, PAX6 and SOX3. Such findings support the hypothesis that hASCs may have a potential usefulness in neurodegenerative conditions. These data can be helpful for the development of new therapeutic approaches in personalized medicine to assess safety and efficacy of the breast reconstruction.https://www.mdpi.com/2073-4409/8/3/282ASCsadipose-derived stem cellsSVFsstromal vascular fraction cellsenzymatic digestionpersonalized medicinecharacteristics of adipose-derived stem cells |
| spellingShingle | Pietro Gentile Maria Serena Piccinno Claudio Calabrese Characteristics and Potentiality of Human Adipose-Derived Stem Cells (hASCs) Obtained from Enzymatic Digestion of Fat Graft Cells ASCs adipose-derived stem cells SVFs stromal vascular fraction cells enzymatic digestion personalized medicine characteristics of adipose-derived stem cells |
| title | Characteristics and Potentiality of Human Adipose-Derived Stem Cells (hASCs) Obtained from Enzymatic Digestion of Fat Graft |
| title_full | Characteristics and Potentiality of Human Adipose-Derived Stem Cells (hASCs) Obtained from Enzymatic Digestion of Fat Graft |
| title_fullStr | Characteristics and Potentiality of Human Adipose-Derived Stem Cells (hASCs) Obtained from Enzymatic Digestion of Fat Graft |
| title_full_unstemmed | Characteristics and Potentiality of Human Adipose-Derived Stem Cells (hASCs) Obtained from Enzymatic Digestion of Fat Graft |
| title_short | Characteristics and Potentiality of Human Adipose-Derived Stem Cells (hASCs) Obtained from Enzymatic Digestion of Fat Graft |
| title_sort | characteristics and potentiality of human adipose derived stem cells hascs obtained from enzymatic digestion of fat graft |
| topic | ASCs adipose-derived stem cells SVFs stromal vascular fraction cells enzymatic digestion personalized medicine characteristics of adipose-derived stem cells |
| url | https://www.mdpi.com/2073-4409/8/3/282 |
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