Ciliostasis of airway epithelial cells facilitates influenza A virus infection
Abstract Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. Treatment of slices with 2% NaCl for 30 min resulted in reversible ciliostasis. When PCLS were infected by a swine influenza virus o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-07-01
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| Series: | Veterinary Research |
| Online Access: | http://link.springer.com/article/10.1186/s13567-018-0568-0 |
| _version_ | 1811323997989634048 |
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| author | Yuguang Fu Jie Tong Fandan Meng Doris Hoeltig Guangliang Liu Xiangping Yin Georg Herrler |
| author_facet | Yuguang Fu Jie Tong Fandan Meng Doris Hoeltig Guangliang Liu Xiangping Yin Georg Herrler |
| author_sort | Yuguang Fu |
| collection | DOAJ |
| description | Abstract Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. Treatment of slices with 2% NaCl for 30 min resulted in reversible ciliostasis. When PCLS were infected by a swine influenza virus of the H3N2 subtype under ciliostatic conditions, the viral yield was about twofold or threefold higher at 24 or 48 h post-infection, respectively, as compared to slices with ciliary activity. Therefore, the cilia beating not only transports the mucus out of the airways, it also impedes virus infection. |
| first_indexed | 2024-04-13T14:06:22Z |
| format | Article |
| id | doaj.art-48e90c6e9ed74319b1a68150d8916fb0 |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-04-13T14:06:22Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-48e90c6e9ed74319b1a68150d8916fb02022-12-22T02:43:54ZengBMCVeterinary Research1297-97162018-07-014911410.1186/s13567-018-0568-0Ciliostasis of airway epithelial cells facilitates influenza A virus infectionYuguang Fu0Jie Tong1Fandan Meng2Doris Hoeltig3Guangliang Liu4Xiangping Yin5Georg Herrler6Institute of Virology, University of Veterinary Medicine Hannover, FoundationInstitute of Virology, University of Veterinary Medicine Hannover, FoundationInstitute of Virology, University of Veterinary Medicine Hannover, FoundationClinic of Swine and Small Ruminants and Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, FoundationLanzhou Veterinary Research Institute, State Key Laboratory of Veterinary Etiological Biology, Chinese Academy of Agricultural SciencesLanzhou Veterinary Research Institute, State Key Laboratory of Veterinary Etiological Biology, Chinese Academy of Agricultural SciencesInstitute of Virology, University of Veterinary Medicine Hannover, FoundationAbstract Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. Treatment of slices with 2% NaCl for 30 min resulted in reversible ciliostasis. When PCLS were infected by a swine influenza virus of the H3N2 subtype under ciliostatic conditions, the viral yield was about twofold or threefold higher at 24 or 48 h post-infection, respectively, as compared to slices with ciliary activity. Therefore, the cilia beating not only transports the mucus out of the airways, it also impedes virus infection.http://link.springer.com/article/10.1186/s13567-018-0568-0 |
| spellingShingle | Yuguang Fu Jie Tong Fandan Meng Doris Hoeltig Guangliang Liu Xiangping Yin Georg Herrler Ciliostasis of airway epithelial cells facilitates influenza A virus infection Veterinary Research |
| title | Ciliostasis of airway epithelial cells facilitates influenza A virus infection |
| title_full | Ciliostasis of airway epithelial cells facilitates influenza A virus infection |
| title_fullStr | Ciliostasis of airway epithelial cells facilitates influenza A virus infection |
| title_full_unstemmed | Ciliostasis of airway epithelial cells facilitates influenza A virus infection |
| title_short | Ciliostasis of airway epithelial cells facilitates influenza A virus infection |
| title_sort | ciliostasis of airway epithelial cells facilitates influenza a virus infection |
| url | http://link.springer.com/article/10.1186/s13567-018-0568-0 |
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