Detection of Trichinella-specific IgE in human Trichinellosis: Creating a new test
Trichinellosis is a parasitic disease of humans caused by the nematode from the genus Trichinella, predominantly Trichinella spiralis (T. spiralis). If Trichinella infection is suspected, based on epidemiological link and clinical criteria within defined period of time, then finding of T...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2014-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2014/0352-51391400065D.pdf |
| _version_ | 1828761280141328384 |
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| author | Dević Marija Gruden-Movsesijan Alisa Sofronić-Milosavljević Ljiljana |
| author_facet | Dević Marija Gruden-Movsesijan Alisa Sofronić-Milosavljević Ljiljana |
| author_sort | Dević Marija |
| collection | DOAJ |
| description | Trichinellosis is a parasitic disease of humans caused by the nematode from
the genus Trichinella, predominantly Trichinella spiralis (T. spiralis). If
Trichinella infection is suspected, based on epidemiological link and
clinical criteria within defined period of time, then finding of
Trichinella-specific antibodies in the examined sera provides a definitive
proof of the infection establishment. Detection of Trichinella-specific IgE
that could precede, coincide or follow IgG seroconversion not only confirms
the infection existence, but could narrow the time frame in which the
infection took place to a year or even less. Since there are no commercially
available tests for monitoring the Trichinella-specific IgE presence during
the course of the disease, our work was aimed to establish this kind of ELISA
test. Specificity and sensitivity of so far described Trichinella-specific
IgE ELISAs are not satisfying enough; two major problems are poor
discrimination between positive and negative results and cross reactivity
with sera of patients with different parasitic diseases. In this study, we
have developed Trichinella-specific IgE Capture ELISA that overcomes problems
with specificity and sensitivity and enables determination of
Trichinella-specific IgE. [Projekat Ministarstva nauke Republike Srbije, br.
173047] |
| first_indexed | 2024-12-11T01:27:24Z |
| format | Article |
| id | doaj.art-48ef69299dd4411ea33c543b17ca58f4 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-12-11T01:27:24Z |
| publishDate | 2014-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-48ef69299dd4411ea33c543b17ca58f42022-12-22T01:25:29ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212014-01-0179121477149010.2298/JSC140402065D0352-51391400065DDetection of Trichinella-specific IgE in human Trichinellosis: Creating a new testDević Marija0Gruden-Movsesijan Alisa1Sofronić-Milosavljević Ljiljana2Institute for the Application of Nuclear Energy - INEP, BelgradeInstitute for the Application of Nuclear Energy - INEP, BelgradeInstitute for the Application of Nuclear Energy - INEP, BelgradeTrichinellosis is a parasitic disease of humans caused by the nematode from the genus Trichinella, predominantly Trichinella spiralis (T. spiralis). If Trichinella infection is suspected, based on epidemiological link and clinical criteria within defined period of time, then finding of Trichinella-specific antibodies in the examined sera provides a definitive proof of the infection establishment. Detection of Trichinella-specific IgE that could precede, coincide or follow IgG seroconversion not only confirms the infection existence, but could narrow the time frame in which the infection took place to a year or even less. Since there are no commercially available tests for monitoring the Trichinella-specific IgE presence during the course of the disease, our work was aimed to establish this kind of ELISA test. Specificity and sensitivity of so far described Trichinella-specific IgE ELISAs are not satisfying enough; two major problems are poor discrimination between positive and negative results and cross reactivity with sera of patients with different parasitic diseases. In this study, we have developed Trichinella-specific IgE Capture ELISA that overcomes problems with specificity and sensitivity and enables determination of Trichinella-specific IgE. [Projekat Ministarstva nauke Republike Srbije, br. 173047]http://www.doiserbia.nb.rs/img/doi/0352-5139/2014/0352-51391400065D.pdfTrichinella spiralisTrichinella-specific IgEcapture ELISA |
| spellingShingle | Dević Marija Gruden-Movsesijan Alisa Sofronić-Milosavljević Ljiljana Detection of Trichinella-specific IgE in human Trichinellosis: Creating a new test Journal of the Serbian Chemical Society Trichinella spiralis Trichinella-specific IgE capture ELISA |
| title | Detection of Trichinella-specific IgE in human Trichinellosis: Creating a new test |
| title_full | Detection of Trichinella-specific IgE in human Trichinellosis: Creating a new test |
| title_fullStr | Detection of Trichinella-specific IgE in human Trichinellosis: Creating a new test |
| title_full_unstemmed | Detection of Trichinella-specific IgE in human Trichinellosis: Creating a new test |
| title_short | Detection of Trichinella-specific IgE in human Trichinellosis: Creating a new test |
| title_sort | detection of trichinella specific ige in human trichinellosis creating a new test |
| topic | Trichinella spiralis Trichinella-specific IgE capture ELISA |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2014/0352-51391400065D.pdf |
| work_keys_str_mv | AT devicmarija detectionoftrichinellaspecificigeinhumantrichinellosiscreatinganewtest AT grudenmovsesijanalisa detectionoftrichinellaspecificigeinhumantrichinellosiscreatinganewtest AT sofronicmilosavljevicljiljana detectionoftrichinellaspecificigeinhumantrichinellosiscreatinganewtest |