Mucosa-Colonizing Microbiota Correlate With Host Autophagy Signaling in Patients With Inflammatory Bowel Disease
Both bacteria and autophagy are implicated in inflammatory bowel disease (IBD) pathogenesis. However, how bacteria crosstalk with autophagy signaling remains largely known, especially in intestinal mucosa. This study aimed to profile the internal complex autophagy signaling cascade and their externa...
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| Format: | Article |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.875238/full |
| _version_ | 1818551313991991296 |
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| author | Wenxue Wang Wenxue Wang Zhongjian Liu Wei Yue Ling Zhu Huijie Zhong Huijie Zhong Chao Yang Tian He Ping Wan Jiawei Geng Jiawei Geng Jiawei Geng |
| author_facet | Wenxue Wang Wenxue Wang Zhongjian Liu Wei Yue Ling Zhu Huijie Zhong Huijie Zhong Chao Yang Tian He Ping Wan Jiawei Geng Jiawei Geng Jiawei Geng |
| author_sort | Wenxue Wang |
| collection | DOAJ |
| description | Both bacteria and autophagy are implicated in inflammatory bowel disease (IBD) pathogenesis. However, how bacteria crosstalk with autophagy signaling remains largely known, especially in intestinal mucosa. This study aimed to profile the internal complex autophagy signaling cascade and their external correlation with these bacteria, and consequently provide a systematic and precise target for future IBD diagnosis and therapy. We found the Ulcerative colitis (UC) patients exhibited more severe dysbiosis than the Crohn’s disease (CD) patients, as represented by alpha diversity, community phenotypes, and functional annotation compared with the control population. Meanwhile, CD patients showed greater transcriptional signaling activities of autophagy, endoplasmic reticulum (ER) stress, and bile acid production. Dominant bacteria (e.g., Rhodococcus, Escherichia, Shigella, and Enterococcus) were positively correlated and low-abundance bacteria (e.g., Bacillus, Acidovorax, Acinetobacter, and Stenotrophomonas) were negatively correlated with the autophagy signaling cascade (184 autophagy genes, 52 ER stress genes, and 22 bile acid production genes). Our observations suggested UC patients showed temporary and widespread microbiota turbulence and CD patients showed processive and local autophagy activity during IBD progression. Intestinal mucosa-colonizing bacteria were correlated with the bile/ER stress/autophagy signaling axis in IBD pathogenesis. |
| first_indexed | 2024-12-12T08:58:14Z |
| format | Article |
| id | doaj.art-48f735792b1e495893c07bd89a3966d6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T08:58:14Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-48f735792b1e495893c07bd89a3966d62022-12-22T00:29:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-05-011310.3389/fmicb.2022.875238875238Mucosa-Colonizing Microbiota Correlate With Host Autophagy Signaling in Patients With Inflammatory Bowel DiseaseWenxue Wang0Wenxue Wang1Zhongjian Liu2Wei Yue3Ling Zhu4Huijie Zhong5Huijie Zhong6Chao Yang7Tian He8Ping Wan9Jiawei Geng10Jiawei Geng11Jiawei Geng12Department of Infectious Disease and Hepatic Disease, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, ChinaSchool of Medicine, Kunming University of Science and Technology, Kunming, ChinaInstitute of Basic and Clinical Medicine, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, ChinaDepartment of Infectious Disease and Hepatic Disease, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, ChinaDepartment of Infectious Disease and Hepatic Disease, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, ChinaDepartment of Infectious Disease and Hepatic Disease, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, ChinaSchool of Medicine, Kunming University of Science and Technology, Kunming, ChinaDepartment of Gastroenterology, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, ChinaDepartment of Gastroenterology, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, ChinaDepartment of Gastroenterology, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, ChinaDepartment of Infectious Disease and Hepatic Disease, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, ChinaSchool of Medicine, Kunming University of Science and Technology, Kunming, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaBoth bacteria and autophagy are implicated in inflammatory bowel disease (IBD) pathogenesis. However, how bacteria crosstalk with autophagy signaling remains largely known, especially in intestinal mucosa. This study aimed to profile the internal complex autophagy signaling cascade and their external correlation with these bacteria, and consequently provide a systematic and precise target for future IBD diagnosis and therapy. We found the Ulcerative colitis (UC) patients exhibited more severe dysbiosis than the Crohn’s disease (CD) patients, as represented by alpha diversity, community phenotypes, and functional annotation compared with the control population. Meanwhile, CD patients showed greater transcriptional signaling activities of autophagy, endoplasmic reticulum (ER) stress, and bile acid production. Dominant bacteria (e.g., Rhodococcus, Escherichia, Shigella, and Enterococcus) were positively correlated and low-abundance bacteria (e.g., Bacillus, Acidovorax, Acinetobacter, and Stenotrophomonas) were negatively correlated with the autophagy signaling cascade (184 autophagy genes, 52 ER stress genes, and 22 bile acid production genes). Our observations suggested UC patients showed temporary and widespread microbiota turbulence and CD patients showed processive and local autophagy activity during IBD progression. Intestinal mucosa-colonizing bacteria were correlated with the bile/ER stress/autophagy signaling axis in IBD pathogenesis.https://www.frontiersin.org/articles/10.3389/fmicb.2022.875238/fullautophagyER stressbilemicrobiometranscriptomeinflammatory bowel disease |
| spellingShingle | Wenxue Wang Wenxue Wang Zhongjian Liu Wei Yue Ling Zhu Huijie Zhong Huijie Zhong Chao Yang Tian He Ping Wan Jiawei Geng Jiawei Geng Jiawei Geng Mucosa-Colonizing Microbiota Correlate With Host Autophagy Signaling in Patients With Inflammatory Bowel Disease Frontiers in Microbiology autophagy ER stress bile microbiome transcriptome inflammatory bowel disease |
| title | Mucosa-Colonizing Microbiota Correlate With Host Autophagy Signaling in Patients With Inflammatory Bowel Disease |
| title_full | Mucosa-Colonizing Microbiota Correlate With Host Autophagy Signaling in Patients With Inflammatory Bowel Disease |
| title_fullStr | Mucosa-Colonizing Microbiota Correlate With Host Autophagy Signaling in Patients With Inflammatory Bowel Disease |
| title_full_unstemmed | Mucosa-Colonizing Microbiota Correlate With Host Autophagy Signaling in Patients With Inflammatory Bowel Disease |
| title_short | Mucosa-Colonizing Microbiota Correlate With Host Autophagy Signaling in Patients With Inflammatory Bowel Disease |
| title_sort | mucosa colonizing microbiota correlate with host autophagy signaling in patients with inflammatory bowel disease |
| topic | autophagy ER stress bile microbiome transcriptome inflammatory bowel disease |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.875238/full |
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