Employing Genome Mining to Unveil a Potential Contribution of Endophytic Bacteria to Antimicrobial Compounds in the <i>Origanum vulgare</i> L. Essential Oil
Essential oils (EOs) from medicinal plants have long been used in traditional medicine for their widely known antimicrobial properties and represent a promising reservoir of bioactive compounds against multidrug-resistant pathogens. Endophytes may contribute to the yield and composition of EOs, repr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/7/1179 |
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| author | Francesco Vitali Arcangela Frascella Giulia Semenzato Sara Del Duca Antonio Palumbo Piccionello Stefano Mocali Renato Fani Giovanni Emiliani |
| author_facet | Francesco Vitali Arcangela Frascella Giulia Semenzato Sara Del Duca Antonio Palumbo Piccionello Stefano Mocali Renato Fani Giovanni Emiliani |
| author_sort | Francesco Vitali |
| collection | DOAJ |
| description | Essential oils (EOs) from medicinal plants have long been used in traditional medicine for their widely known antimicrobial properties and represent a promising reservoir of bioactive compounds against multidrug-resistant pathogens. Endophytes may contribute to the yield and composition of EOs, representing a useful tool for biotechnological applications. In this work, we investigated the genomic basis of this potential contribution. The annotated genomes of four endophytic strains isolated from <i>Origanum vulgare</i> L. were used to obtain KEGG ortholog codes, which were used for the annotation of different pathways in KEGG, and to evaluate whether endophytes might harbor the (complete) gene sets for terpene and/or plant hormone biosynthesis. All strains possessed ortholog genes for the mevalonate-independent pathway (MEP/DOXP), allowing for the production of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) precursors. Ortholog genes for the next steps in terpenoid biosynthesis were scarce. All the strains possess potential plant growth promotion (PGP) ability, as shown by the presence of orthologous genes involved in the biosynthesis of indoleacetic acid. The main contribution of endophytes to the yield and composition of <i>O. vulgare</i> EO very likely resides in their PGP activities and in the biosynthesis of precursors of bioactive compounds. |
| first_indexed | 2024-03-11T01:21:28Z |
| format | Article |
| id | doaj.art-48f9b906b80840bd9be6be93da48f845 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T01:21:28Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-48f9b906b80840bd9be6be93da48f8452023-11-18T18:03:23ZengMDPI AGAntibiotics2079-63822023-07-01127117910.3390/antibiotics12071179Employing Genome Mining to Unveil a Potential Contribution of Endophytic Bacteria to Antimicrobial Compounds in the <i>Origanum vulgare</i> L. Essential OilFrancesco Vitali0Arcangela Frascella1Giulia Semenzato2Sara Del Duca3Antonio Palumbo Piccionello4Stefano Mocali5Renato Fani6Giovanni Emiliani7Research Centre for Agriculture and Environment, Council for Agricultural Research and Economics (CREA-AA), Via di Lanciola 12/A, 50125 Cascine del Riccio, ItalyInstitute for Sustainable Plant Protection (IPSP), National Research Council (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, 50019 Sesto Fiorentino, ItalyResearch Centre for Agriculture and Environment, Council for Agricultural Research and Economics (CREA-AA), Via di Lanciola 12/A, 50125 Cascine del Riccio, ItalyDepartment of Biological Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Viale delle Scienze Ed.17, 90128 Palermo, ItalyResearch Centre for Agriculture and Environment, Council for Agricultural Research and Economics (CREA-AA), Via di Lanciola 12/A, 50125 Cascine del Riccio, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, 50019 Sesto Fiorentino, ItalyInstitute for Sustainable Plant Protection (IPSP), National Research Council (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, ItalyEssential oils (EOs) from medicinal plants have long been used in traditional medicine for their widely known antimicrobial properties and represent a promising reservoir of bioactive compounds against multidrug-resistant pathogens. Endophytes may contribute to the yield and composition of EOs, representing a useful tool for biotechnological applications. In this work, we investigated the genomic basis of this potential contribution. The annotated genomes of four endophytic strains isolated from <i>Origanum vulgare</i> L. were used to obtain KEGG ortholog codes, which were used for the annotation of different pathways in KEGG, and to evaluate whether endophytes might harbor the (complete) gene sets for terpene and/or plant hormone biosynthesis. All strains possessed ortholog genes for the mevalonate-independent pathway (MEP/DOXP), allowing for the production of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) precursors. Ortholog genes for the next steps in terpenoid biosynthesis were scarce. All the strains possess potential plant growth promotion (PGP) ability, as shown by the presence of orthologous genes involved in the biosynthesis of indoleacetic acid. The main contribution of endophytes to the yield and composition of <i>O. vulgare</i> EO very likely resides in their PGP activities and in the biosynthesis of precursors of bioactive compounds.https://www.mdpi.com/2079-6382/12/7/1179VOCsendophytemedicinal plantsbiosynthesis pathways |
| spellingShingle | Francesco Vitali Arcangela Frascella Giulia Semenzato Sara Del Duca Antonio Palumbo Piccionello Stefano Mocali Renato Fani Giovanni Emiliani Employing Genome Mining to Unveil a Potential Contribution of Endophytic Bacteria to Antimicrobial Compounds in the <i>Origanum vulgare</i> L. Essential Oil Antibiotics VOCs endophyte medicinal plants biosynthesis pathways |
| title | Employing Genome Mining to Unveil a Potential Contribution of Endophytic Bacteria to Antimicrobial Compounds in the <i>Origanum vulgare</i> L. Essential Oil |
| title_full | Employing Genome Mining to Unveil a Potential Contribution of Endophytic Bacteria to Antimicrobial Compounds in the <i>Origanum vulgare</i> L. Essential Oil |
| title_fullStr | Employing Genome Mining to Unveil a Potential Contribution of Endophytic Bacteria to Antimicrobial Compounds in the <i>Origanum vulgare</i> L. Essential Oil |
| title_full_unstemmed | Employing Genome Mining to Unveil a Potential Contribution of Endophytic Bacteria to Antimicrobial Compounds in the <i>Origanum vulgare</i> L. Essential Oil |
| title_short | Employing Genome Mining to Unveil a Potential Contribution of Endophytic Bacteria to Antimicrobial Compounds in the <i>Origanum vulgare</i> L. Essential Oil |
| title_sort | employing genome mining to unveil a potential contribution of endophytic bacteria to antimicrobial compounds in the i origanum vulgare i l essential oil |
| topic | VOCs endophyte medicinal plants biosynthesis pathways |
| url | https://www.mdpi.com/2079-6382/12/7/1179 |
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