Development of apigenin loaded gastroretentive microsponge for the targeting of Helicobacter pylori
The goal of the present work was to invent an apigenin-stacked gastroretentive microsponge to target H. pylori. The quasi-emulsion technique was used to prepare microsponges, which were then tested for various physicochemical properties, in-vivo gastric retention, and in-vitro anti-H. pylori study....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Saudi Pharmaceutical Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016423000567 |
| _version_ | 1797834733185400832 |
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| author | Mohammed Jafar Mohd Sajjad Ahmad Khan Mohammed Salahuddin Saira Zahoor Hanan MohammedHesham Slais Layali Ibrahim Alalwan Heba Radhi Alshaban |
| author_facet | Mohammed Jafar Mohd Sajjad Ahmad Khan Mohammed Salahuddin Saira Zahoor Hanan MohammedHesham Slais Layali Ibrahim Alalwan Heba Radhi Alshaban |
| author_sort | Mohammed Jafar |
| collection | DOAJ |
| description | The goal of the present work was to invent an apigenin-stacked gastroretentive microsponge to target H. pylori. The quasi-emulsion technique was used to prepare microsponges, which were then tested for various physicochemical properties, in-vivo gastric retention, and in-vitro anti-H. pylori study. The microsponge that demonstrated a comparatively good product yield (76.23 ± 0.84), excellent entrapment efficiency (97.84 ± 0.85), sustained in-vitro gastric retention period, and prolonged drug release were chosen for further investigations. The microsponge's SEM analysis showed that it had a spherical form, porous surface, and interconnected spaces. No drug-polymer interactions were detected in the FTIR investigation. Apigenin was found to be dispersed in the microsponge's polymeric matrix according to DSC & XRD investigations. Moreover, the microsponge in the rat's stomach floated for 4 h, according to the ultrasonography. The antibacterial activity of apigenin against H. pylori was nearly two folds more than the pure apigenin and had a more sustained release in the best microsponge, according to the in vitro MIC data, when compared to pure apigenin. To sum up, the developed gastroretentive microsponge with apigenin offers a viable alternative for the efficient targeting of H. pylori. But more preclinical & clinical studies of our best microsponge would yield considerably more fruitful results. |
| first_indexed | 2024-04-09T14:43:18Z |
| format | Article |
| id | doaj.art-48febcd84d9f479e81b5f5753cbe2c22 |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-04-09T14:43:18Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-48febcd84d9f479e81b5f5753cbe2c222023-05-03T04:09:42ZengElsevierSaudi Pharmaceutical Journal1319-01642023-05-01315659668Development of apigenin loaded gastroretentive microsponge for the targeting of Helicobacter pyloriMohammed Jafar0Mohd Sajjad Ahmad Khan1Mohammed Salahuddin2Saira Zahoor3Hanan MohammedHesham Slais4Layali Ibrahim Alalwan5Heba Radhi Alshaban6Department of Pharmaceutics, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 34212, Saudi Arabia; Corresponding author at: Department of Pharmaceutics, College of Clinical Pharmacy, Dammam, Saudi Arabia.Department of Basic Sciences, Deanship of Preparatory Year and Supporting Studies, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 34212, Saudi ArabiaDepartment of Clinical Pharmacy Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 34212, Saudi ArabiaDepartment of Pharmaceutics, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 34212, Saudi ArabiaDepartment of Pharmaceutics, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 34212, Saudi ArabiaDepartment of Pharmaceutics, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 34212, Saudi ArabiaDepartment of Pharmaceutics, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 34212, Saudi ArabiaThe goal of the present work was to invent an apigenin-stacked gastroretentive microsponge to target H. pylori. The quasi-emulsion technique was used to prepare microsponges, which were then tested for various physicochemical properties, in-vivo gastric retention, and in-vitro anti-H. pylori study. The microsponge that demonstrated a comparatively good product yield (76.23 ± 0.84), excellent entrapment efficiency (97.84 ± 0.85), sustained in-vitro gastric retention period, and prolonged drug release were chosen for further investigations. The microsponge's SEM analysis showed that it had a spherical form, porous surface, and interconnected spaces. No drug-polymer interactions were detected in the FTIR investigation. Apigenin was found to be dispersed in the microsponge's polymeric matrix according to DSC & XRD investigations. Moreover, the microsponge in the rat's stomach floated for 4 h, according to the ultrasonography. The antibacterial activity of apigenin against H. pylori was nearly two folds more than the pure apigenin and had a more sustained release in the best microsponge, according to the in vitro MIC data, when compared to pure apigenin. To sum up, the developed gastroretentive microsponge with apigenin offers a viable alternative for the efficient targeting of H. pylori. But more preclinical & clinical studies of our best microsponge would yield considerably more fruitful results.http://www.sciencedirect.com/science/article/pii/S1319016423000567ApigeninFlavonoidH. pylori bacteriaBuoyancyMicroencapsulationIn-vivo activity |
| spellingShingle | Mohammed Jafar Mohd Sajjad Ahmad Khan Mohammed Salahuddin Saira Zahoor Hanan MohammedHesham Slais Layali Ibrahim Alalwan Heba Radhi Alshaban Development of apigenin loaded gastroretentive microsponge for the targeting of Helicobacter pylori Saudi Pharmaceutical Journal Apigenin Flavonoid H. pylori bacteria Buoyancy Microencapsulation In-vivo activity |
| title | Development of apigenin loaded gastroretentive microsponge for the targeting of Helicobacter pylori |
| title_full | Development of apigenin loaded gastroretentive microsponge for the targeting of Helicobacter pylori |
| title_fullStr | Development of apigenin loaded gastroretentive microsponge for the targeting of Helicobacter pylori |
| title_full_unstemmed | Development of apigenin loaded gastroretentive microsponge for the targeting of Helicobacter pylori |
| title_short | Development of apigenin loaded gastroretentive microsponge for the targeting of Helicobacter pylori |
| title_sort | development of apigenin loaded gastroretentive microsponge for the targeting of helicobacter pylori |
| topic | Apigenin Flavonoid H. pylori bacteria Buoyancy Microencapsulation In-vivo activity |
| url | http://www.sciencedirect.com/science/article/pii/S1319016423000567 |
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