Prevalence of β-lactamase-encoding genes and molecular typing of Acinetobacter baumannii isolates carrying carbapenemase OXA-24 in children
Abstract Background β-Lactam antibiotics have been broadly used for the treatment of Acinetobacter baumannii infections, resulting in development of β-lactam inactivating β-lactamases. Here, we described antibiotic resistance rate, prevalence of β-lactamase-encoding genes, and clonal relationships o...
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| Format: | Article |
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BMC
2021-10-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
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| Online Access: | https://doi.org/10.1186/s12941-021-00480-5 |
| _version_ | 1818935286687596544 |
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| author | Neda Yousefi Nojookambari Mehrzad Sadredinamin Razieh Dehbanipour Zohreh Ghalavand Gita Eslami Maryam Vaezjalali Bahram Nikmanesh Sajjad Yazdansetad |
| author_facet | Neda Yousefi Nojookambari Mehrzad Sadredinamin Razieh Dehbanipour Zohreh Ghalavand Gita Eslami Maryam Vaezjalali Bahram Nikmanesh Sajjad Yazdansetad |
| author_sort | Neda Yousefi Nojookambari |
| collection | DOAJ |
| description | Abstract Background β-Lactam antibiotics have been broadly used for the treatment of Acinetobacter baumannii infections, resulting in development of β-lactam inactivating β-lactamases. Here, we described antibiotic resistance rate, prevalence of β-lactamase-encoding genes, and clonal relationships of A. baumannii strains isolated from children referred to Children’s Medical Center in Tehran, Iran, during 2019–2020. Methods A total of 60 non-replicate A. baumannii isolates were recovered from clinical specimens of pediatric patients. Antibiotic susceptibility testing was done by the disc diffusion method. Colistin susceptibility of isolates was performed by the broth microdilution method. β-lactamase-encoding genes were characterized by PCR. The presence of ISAba1 element upstream of the several oxacillinase genes was also checked. Genetic relatedness of isolates was determined by using random amplification of polymorphic DNA (RAPD) typing. Results The antimicrobial susceptibility tests showed that 83.3% of A. baumannii isolates were MDR, and 40% XDR. Both MDR and XDR A. baumannii isolates were susceptible to colistin. The frequency of bla OXA-51-like, bla OXA-23-like, bla TEM, bla OXA-24-like, bla PER, bla SHV, bla CTX-M, bla OXA-58-like, and bla IMP was 100, 93.33, 60, 36.67, 28.33, 8.33, 5, 3.33, and 1.67%, respectively. Coexistence of ISAba1/bla OXA-23-like and ISAba1/bla OXA-51-like was observed in 65% and 85% of isolates, respectively. RAPD analysis revealed 4 common types and 2 single types of A. baumannii isolates. Conclusions The multiple clones harboring bla OXA-23-like, ISAba1-bla OXA-51-like, and ISAba1-bla OXA-23-like were responsible for the spread of A. baumannii isolates in our clinical wards. Dissemination of the well-established clones is worrisome and would become therapeutic challenges due to the possible transferring genetic elements associated with resistance. |
| first_indexed | 2024-12-20T05:17:46Z |
| format | Article |
| id | doaj.art-4908dabd4f884678ab7cd8cb63892b90 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-12-20T05:17:46Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-4908dabd4f884678ab7cd8cb63892b902022-12-21T19:52:07ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112021-10-012011810.1186/s12941-021-00480-5Prevalence of β-lactamase-encoding genes and molecular typing of Acinetobacter baumannii isolates carrying carbapenemase OXA-24 in childrenNeda Yousefi Nojookambari0Mehrzad Sadredinamin1Razieh Dehbanipour2Zohreh Ghalavand3Gita Eslami4Maryam Vaezjalali5Bahram Nikmanesh6Sajjad Yazdansetad7Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical SciencesLaboratory Sciences Research Center, Golestan University of Medical SciencesAbstract Background β-Lactam antibiotics have been broadly used for the treatment of Acinetobacter baumannii infections, resulting in development of β-lactam inactivating β-lactamases. Here, we described antibiotic resistance rate, prevalence of β-lactamase-encoding genes, and clonal relationships of A. baumannii strains isolated from children referred to Children’s Medical Center in Tehran, Iran, during 2019–2020. Methods A total of 60 non-replicate A. baumannii isolates were recovered from clinical specimens of pediatric patients. Antibiotic susceptibility testing was done by the disc diffusion method. Colistin susceptibility of isolates was performed by the broth microdilution method. β-lactamase-encoding genes were characterized by PCR. The presence of ISAba1 element upstream of the several oxacillinase genes was also checked. Genetic relatedness of isolates was determined by using random amplification of polymorphic DNA (RAPD) typing. Results The antimicrobial susceptibility tests showed that 83.3% of A. baumannii isolates were MDR, and 40% XDR. Both MDR and XDR A. baumannii isolates were susceptible to colistin. The frequency of bla OXA-51-like, bla OXA-23-like, bla TEM, bla OXA-24-like, bla PER, bla SHV, bla CTX-M, bla OXA-58-like, and bla IMP was 100, 93.33, 60, 36.67, 28.33, 8.33, 5, 3.33, and 1.67%, respectively. Coexistence of ISAba1/bla OXA-23-like and ISAba1/bla OXA-51-like was observed in 65% and 85% of isolates, respectively. RAPD analysis revealed 4 common types and 2 single types of A. baumannii isolates. Conclusions The multiple clones harboring bla OXA-23-like, ISAba1-bla OXA-51-like, and ISAba1-bla OXA-23-like were responsible for the spread of A. baumannii isolates in our clinical wards. Dissemination of the well-established clones is worrisome and would become therapeutic challenges due to the possible transferring genetic elements associated with resistance.https://doi.org/10.1186/s12941-021-00480-5Antimicrobial susceptibilityPediatricsAcinetobacter baumanniiRAPD-PCRβ-Lactamasesbla OXA-24-like |
| spellingShingle | Neda Yousefi Nojookambari Mehrzad Sadredinamin Razieh Dehbanipour Zohreh Ghalavand Gita Eslami Maryam Vaezjalali Bahram Nikmanesh Sajjad Yazdansetad Prevalence of β-lactamase-encoding genes and molecular typing of Acinetobacter baumannii isolates carrying carbapenemase OXA-24 in children Annals of Clinical Microbiology and Antimicrobials Antimicrobial susceptibility Pediatrics Acinetobacter baumannii RAPD-PCR β-Lactamases bla OXA-24-like |
| title | Prevalence of β-lactamase-encoding genes and molecular typing of Acinetobacter baumannii isolates carrying carbapenemase OXA-24 in children |
| title_full | Prevalence of β-lactamase-encoding genes and molecular typing of Acinetobacter baumannii isolates carrying carbapenemase OXA-24 in children |
| title_fullStr | Prevalence of β-lactamase-encoding genes and molecular typing of Acinetobacter baumannii isolates carrying carbapenemase OXA-24 in children |
| title_full_unstemmed | Prevalence of β-lactamase-encoding genes and molecular typing of Acinetobacter baumannii isolates carrying carbapenemase OXA-24 in children |
| title_short | Prevalence of β-lactamase-encoding genes and molecular typing of Acinetobacter baumannii isolates carrying carbapenemase OXA-24 in children |
| title_sort | prevalence of β lactamase encoding genes and molecular typing of acinetobacter baumannii isolates carrying carbapenemase oxa 24 in children |
| topic | Antimicrobial susceptibility Pediatrics Acinetobacter baumannii RAPD-PCR β-Lactamases bla OXA-24-like |
| url | https://doi.org/10.1186/s12941-021-00480-5 |
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