Garlic, Cilantro and Chlorella’s Effect on Intestine Histoarchitecture Changes in Cd-Intoxicated Prussian Carp (Carassius gibelio)
Bioactive compounds from natural sources can act as oxygen free radical scavengers or metal chelators, which enables them to be used as natural antagonists to heavy metals toxicity. So the present study was carried out to compare histologically the aspect of intestine tissue of Prussian carp specime...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Agroprint Timisoara
2023-09-01
|
| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/387 |
| _version_ | 1797214747685289984 |
|---|---|
| author | Marioara Nicula Gabi Dumitrescu Nicolae Păcală Lavinia Ștef Camelia Tulcan Ioan Bencsik Monica Dragomirescu Dorel Dronca Liliana Petculescu-Ciochina Ioan Peț Ion Carabă Silvia Erina Adela Marcu |
| author_facet | Marioara Nicula Gabi Dumitrescu Nicolae Păcală Lavinia Ștef Camelia Tulcan Ioan Bencsik Monica Dragomirescu Dorel Dronca Liliana Petculescu-Ciochina Ioan Peț Ion Carabă Silvia Erina Adela Marcu |
| author_sort | Marioara Nicula |
| collection | DOAJ |
| description | Bioactive compounds from natural sources can act as oxygen free radical scavengers or metal chelators, which enables them to be used as natural antagonists to heavy metals toxicity. So the present study was carried out to compare histologically the aspect of intestine tissue of Prussian carp specimens, subjected to chronic Cd intoxication with and without garlic, cilantro and chlorella dietary supplementation.150 Prussian carps, with weight of 10-12 g were divided according to the following treatments for 21 days: C (without treatment), E1 (10 ppm Cd into water), E2 (10 ppm Cd into water+2% lyophilized garlic in feed), E3 (10 ppm Cd into water+2% lyophilized cilantro in feed), E4 (10 ppm Cd into water+2% lyophilized chlorella in feed). Cadmium toxicity and the potential protective effect of the three lyophilized products against the impact of cadmium toxicity were histopathologically assessed. For this purpose, fragments of intestine were removed and routinely processed at the end of experimental period and analyzed in light microscopy. A specific QuickPHOTO Micro 2.2 software has been used for the histological study. Tissue alterations were assessed using the histopathological score ranging from – to +++ depending on the degree and extend of lesions: (-) none, (+) mild occurrence, (++) moderate occurrence, (+++) severe occurrence. Our research findings show that Cd induces a significant increase in histopathological changes like vascular network hypertrophies and reach infiltrating leukocyte cells. In the same time, chlorella powder added to the fish diet, expressed the most effectiveness on the intestinal recovery of the cadmium-intoxicated fish followed by while cilantro and garlic powder. |
| first_indexed | 2024-04-24T11:19:05Z |
| format | Article |
| id | doaj.art-490c0b235f73411aa92942feb9c9fed3 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:19:05Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-490c0b235f73411aa92942feb9c9fed32024-04-11T04:50:00ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-01492122122387Garlic, Cilantro and Chlorella’s Effect on Intestine Histoarchitecture Changes in Cd-Intoxicated Prussian Carp (Carassius gibelio)Marioara Nicula0Gabi Dumitrescu1Nicolae Păcală2Lavinia Ștef3Camelia Tulcan4Ioan Bencsik5Monica Dragomirescu6Dorel Dronca7Liliana Petculescu-Ciochina8Ioan Peț9Ion Carabă10Silvia Erina11Adela Marcu12Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timişoara, 300645-Timişoara, Calea Aradului 119, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timişoara, 300645-Timişoara, Calea Aradului 119, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timişoara, 300645-Timişoara, Calea Aradului 119, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timişoara, 300645-Timişoara, Calea Aradului 119, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timişoara, 300645-Timişoara, Calea Aradului 119, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timişoara, 300645-Timişoara, Calea Aradului 119, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timişoara, 300645-Timişoara, Calea Aradului 119, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timişoara, 300645-Timişoara, Calea Aradului 119, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timişoara, 300645-Timişoara, Calea Aradului 119, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timişoara, 300645-Timişoara, Calea Aradului 119, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timişoara, 300645-Timişoara, Calea Aradului 119, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timişoara, 300645-Timişoara, Calea Aradului 119, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timişoara, 300645-Timişoara, Calea Aradului 119, RomaniaBioactive compounds from natural sources can act as oxygen free radical scavengers or metal chelators, which enables them to be used as natural antagonists to heavy metals toxicity. So the present study was carried out to compare histologically the aspect of intestine tissue of Prussian carp specimens, subjected to chronic Cd intoxication with and without garlic, cilantro and chlorella dietary supplementation.150 Prussian carps, with weight of 10-12 g were divided according to the following treatments for 21 days: C (without treatment), E1 (10 ppm Cd into water), E2 (10 ppm Cd into water+2% lyophilized garlic in feed), E3 (10 ppm Cd into water+2% lyophilized cilantro in feed), E4 (10 ppm Cd into water+2% lyophilized chlorella in feed). Cadmium toxicity and the potential protective effect of the three lyophilized products against the impact of cadmium toxicity were histopathologically assessed. For this purpose, fragments of intestine were removed and routinely processed at the end of experimental period and analyzed in light microscopy. A specific QuickPHOTO Micro 2.2 software has been used for the histological study. Tissue alterations were assessed using the histopathological score ranging from – to +++ depending on the degree and extend of lesions: (-) none, (+) mild occurrence, (++) moderate occurrence, (+++) severe occurrence. Our research findings show that Cd induces a significant increase in histopathological changes like vascular network hypertrophies and reach infiltrating leukocyte cells. In the same time, chlorella powder added to the fish diet, expressed the most effectiveness on the intestinal recovery of the cadmium-intoxicated fish followed by while cilantro and garlic powder.https://spasb.ro/index.php/public_html/article/view/387cadmium toxicityfishintestine histopathologylyophilizationnatural chelators |
| spellingShingle | Marioara Nicula Gabi Dumitrescu Nicolae Păcală Lavinia Ștef Camelia Tulcan Ioan Bencsik Monica Dragomirescu Dorel Dronca Liliana Petculescu-Ciochina Ioan Peț Ion Carabă Silvia Erina Adela Marcu Garlic, Cilantro and Chlorella’s Effect on Intestine Histoarchitecture Changes in Cd-Intoxicated Prussian Carp (Carassius gibelio) Scientific Papers Animal Science and Biotechnologies cadmium toxicity fish intestine histopathology lyophilization natural chelators |
| title | Garlic, Cilantro and Chlorella’s Effect on Intestine Histoarchitecture Changes in Cd-Intoxicated Prussian Carp (Carassius gibelio) |
| title_full | Garlic, Cilantro and Chlorella’s Effect on Intestine Histoarchitecture Changes in Cd-Intoxicated Prussian Carp (Carassius gibelio) |
| title_fullStr | Garlic, Cilantro and Chlorella’s Effect on Intestine Histoarchitecture Changes in Cd-Intoxicated Prussian Carp (Carassius gibelio) |
| title_full_unstemmed | Garlic, Cilantro and Chlorella’s Effect on Intestine Histoarchitecture Changes in Cd-Intoxicated Prussian Carp (Carassius gibelio) |
| title_short | Garlic, Cilantro and Chlorella’s Effect on Intestine Histoarchitecture Changes in Cd-Intoxicated Prussian Carp (Carassius gibelio) |
| title_sort | garlic cilantro and chlorella s effect on intestine histoarchitecture changes in cd intoxicated prussian carp carassius gibelio |
| topic | cadmium toxicity fish intestine histopathology lyophilization natural chelators |
| url | https://spasb.ro/index.php/public_html/article/view/387 |
| work_keys_str_mv | AT marioaranicula garliccilantroandchlorellaseffectonintestinehistoarchitecturechangesincdintoxicatedprussiancarpcarassiusgibelio AT gabidumitrescu garliccilantroandchlorellaseffectonintestinehistoarchitecturechangesincdintoxicatedprussiancarpcarassiusgibelio AT nicolaepacala garliccilantroandchlorellaseffectonintestinehistoarchitecturechangesincdintoxicatedprussiancarpcarassiusgibelio AT laviniastef garliccilantroandchlorellaseffectonintestinehistoarchitecturechangesincdintoxicatedprussiancarpcarassiusgibelio AT cameliatulcan garliccilantroandchlorellaseffectonintestinehistoarchitecturechangesincdintoxicatedprussiancarpcarassiusgibelio AT ioanbencsik garliccilantroandchlorellaseffectonintestinehistoarchitecturechangesincdintoxicatedprussiancarpcarassiusgibelio AT monicadragomirescu garliccilantroandchlorellaseffectonintestinehistoarchitecturechangesincdintoxicatedprussiancarpcarassiusgibelio AT doreldronca garliccilantroandchlorellaseffectonintestinehistoarchitecturechangesincdintoxicatedprussiancarpcarassiusgibelio AT lilianapetculescuciochina garliccilantroandchlorellaseffectonintestinehistoarchitecturechangesincdintoxicatedprussiancarpcarassiusgibelio AT ioanpet garliccilantroandchlorellaseffectonintestinehistoarchitecturechangesincdintoxicatedprussiancarpcarassiusgibelio AT ioncaraba garliccilantroandchlorellaseffectonintestinehistoarchitecturechangesincdintoxicatedprussiancarpcarassiusgibelio AT silviaerina garliccilantroandchlorellaseffectonintestinehistoarchitecturechangesincdintoxicatedprussiancarpcarassiusgibelio AT adelamarcu garliccilantroandchlorellaseffectonintestinehistoarchitecturechangesincdintoxicatedprussiancarpcarassiusgibelio |