Modeling Cardiomyopathies in a Dish: State-of-the-Art and Novel Perspectives on hiPSC-Derived Cardiomyocytes Maturation
The stem cell technology and the induced pluripotent stem cells (iPSCs) production represent an excellent alternative tool to study cardiomyopathies, which overcome the limitations associated with primary cardiomyocytes (CMs) access and manipulation. CMs from human iPSCs (hiPSC–CMs) are genetically...
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MDPI AG
2021-07-01
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author | Francesco Lodola Verónica Celeste De Giusti Claudia Maniezzi Daniele Martone Ilaria Stadiotti Elena Sommariva Angela Serena Maione |
author_facet | Francesco Lodola Verónica Celeste De Giusti Claudia Maniezzi Daniele Martone Ilaria Stadiotti Elena Sommariva Angela Serena Maione |
author_sort | Francesco Lodola |
collection | DOAJ |
description | The stem cell technology and the induced pluripotent stem cells (iPSCs) production represent an excellent alternative tool to study cardiomyopathies, which overcome the limitations associated with primary cardiomyocytes (CMs) access and manipulation. CMs from human iPSCs (hiPSC–CMs) are genetically identical to patient primary cells of origin, with the main electrophysiological and mechanical features of CMs. The key issue to be solved is to achieve a degree of structural and functional maturity typical of adult CMs. In this perspective, we will focus on the main differences between fetal-like hiPSC-CMs and adult CMs. A viewpoint is given on the different approaches used to improve hiPSC-CMs maturity, spanning from long-term culture to complex engineered heart tissue. Further, we outline limitations and future developments needed in cardiomyopathy disease modeling. |
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issn | 2079-7737 |
language | English |
last_indexed | 2024-03-10T09:00:15Z |
publishDate | 2021-07-01 |
publisher | MDPI AG |
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spelling | doaj.art-490e122b730f4b499edb7f68985ec0362023-11-22T06:49:43ZengMDPI AGBiology2079-77372021-07-0110873010.3390/biology10080730Modeling Cardiomyopathies in a Dish: State-of-the-Art and Novel Perspectives on hiPSC-Derived Cardiomyocytes MaturationFrancesco Lodola0Verónica Celeste De Giusti1Claudia Maniezzi2Daniele Martone3Ilaria Stadiotti4Elena Sommariva5Angela Serena Maione6Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza, 2, Ed U3, 20126 Milan, ItalyCentro de Investigaciones Cardiovasculares, Facultad de Ciencias Médicas, Universidad Nacional de La Plata-CONICET, La Plata 1900, ArgentinaDepartment of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza, 2, Ed U3, 20126 Milan, ItalyDepartment of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza, 2, Ed U3, 20126 Milan, ItalyVascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino IRCCS, 20138 Milan, ItalyVascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino IRCCS, 20138 Milan, ItalyVascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino IRCCS, 20138 Milan, ItalyThe stem cell technology and the induced pluripotent stem cells (iPSCs) production represent an excellent alternative tool to study cardiomyopathies, which overcome the limitations associated with primary cardiomyocytes (CMs) access and manipulation. CMs from human iPSCs (hiPSC–CMs) are genetically identical to patient primary cells of origin, with the main electrophysiological and mechanical features of CMs. The key issue to be solved is to achieve a degree of structural and functional maturity typical of adult CMs. In this perspective, we will focus on the main differences between fetal-like hiPSC-CMs and adult CMs. A viewpoint is given on the different approaches used to improve hiPSC-CMs maturity, spanning from long-term culture to complex engineered heart tissue. Further, we outline limitations and future developments needed in cardiomyopathy disease modeling.https://www.mdpi.com/2079-7737/10/8/730hiPSC-CMscardiomyopathiescardiovascular disease modelingpatient-specific medicinestem cell maturation |
spellingShingle | Francesco Lodola Verónica Celeste De Giusti Claudia Maniezzi Daniele Martone Ilaria Stadiotti Elena Sommariva Angela Serena Maione Modeling Cardiomyopathies in a Dish: State-of-the-Art and Novel Perspectives on hiPSC-Derived Cardiomyocytes Maturation Biology hiPSC-CMs cardiomyopathies cardiovascular disease modeling patient-specific medicine stem cell maturation |
title | Modeling Cardiomyopathies in a Dish: State-of-the-Art and Novel Perspectives on hiPSC-Derived Cardiomyocytes Maturation |
title_full | Modeling Cardiomyopathies in a Dish: State-of-the-Art and Novel Perspectives on hiPSC-Derived Cardiomyocytes Maturation |
title_fullStr | Modeling Cardiomyopathies in a Dish: State-of-the-Art and Novel Perspectives on hiPSC-Derived Cardiomyocytes Maturation |
title_full_unstemmed | Modeling Cardiomyopathies in a Dish: State-of-the-Art and Novel Perspectives on hiPSC-Derived Cardiomyocytes Maturation |
title_short | Modeling Cardiomyopathies in a Dish: State-of-the-Art and Novel Perspectives on hiPSC-Derived Cardiomyocytes Maturation |
title_sort | modeling cardiomyopathies in a dish state of the art and novel perspectives on hipsc derived cardiomyocytes maturation |
topic | hiPSC-CMs cardiomyopathies cardiovascular disease modeling patient-specific medicine stem cell maturation |
url | https://www.mdpi.com/2079-7737/10/8/730 |
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