Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding
The substitution of more appreciated animal species by animal species of lower commercial value is a common type of meat product adulteration. DNA metabarcoding, the combination of DNA barcoding with next-generation sequencing (NGS), plays an increasing role in food authentication. In the present st...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/11/2875 |
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| author | Laura Preckel Claudia Brünen-Nieweler Grégoire Denay Henning Petersen Margit Cichna-Markl Stefanie Dobrovolny Rupert Hochegger |
| author_facet | Laura Preckel Claudia Brünen-Nieweler Grégoire Denay Henning Petersen Margit Cichna-Markl Stefanie Dobrovolny Rupert Hochegger |
| author_sort | Laura Preckel |
| collection | DOAJ |
| description | The substitution of more appreciated animal species by animal species of lower commercial value is a common type of meat product adulteration. DNA metabarcoding, the combination of DNA barcoding with next-generation sequencing (NGS), plays an increasing role in food authentication. In the present study, we investigated the applicability of a DNA metabarcoding method for routine analysis of mammalian and poultry species in food and pet food products. We analyzed a total of 104 samples (25 reference samples, 56 food products and 23 pet food products) by DNA metabarcoding and by using a commercial DNA array and/or by real-time PCR. The qualitative and quantitative results obtained by the DNA metabarcoding method were in line with those obtained by PCR. Results from the independent analysis of a subset of seven reference samples in two laboratories demonstrate the robustness and reproducibility of the DNA metabarcoding method. DNA metabarcoding is particularly suitable for detecting unexpected species ignored by targeted methods such as real-time PCR and can also be an attractive alternative with respect to the expenses as indicated by current data from the cost accounting of the AGES laboratory. Our results for the commercial samples show that in addition to food products, DNA metabarcoding is particularly applicable to pet food products, which frequently contain multiple animal species and are also highly prone to adulteration as indicated by the high portion of analyzed pet food products containing undeclared species. |
| first_indexed | 2024-03-10T05:29:25Z |
| format | Article |
| id | doaj.art-4910bbc3efeb4cfd851dcd7d0881fa1f |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T05:29:25Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-4910bbc3efeb4cfd851dcd7d0881fa1f2023-11-22T23:23:19ZengMDPI AGFoods2304-81582021-11-011011287510.3390/foods10112875Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA MetabarcodingLaura Preckel0Claudia Brünen-Nieweler1Grégoire Denay2Henning Petersen3Margit Cichna-Markl4Stefanie Dobrovolny5Rupert Hochegger6Chemical and Veterinary Analytical Institute Muensterland-Emscher-Lippe (CVUA-MEL), Joseph-Koenig-Strasse 40, 48147 Muenster, GermanyChemical and Veterinary Analytical Institute Muensterland-Emscher-Lippe (CVUA-MEL), Joseph-Koenig-Strasse 40, 48147 Muenster, GermanyChemical and Veterinary Analytical Institute Rhein-Ruhr-Wupper (CVUA-RRW), Deutscher Ring 100, 47798 Krefeld, GermanyChemical and Veterinary Analytical Institute Ostwestfalen-Lippe (CVUA-OWL), Westerfeldstrasse 1, 32758 Detmold, GermanyDepartment of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Währinger Strasse 38, 1090 Vienna, AustriaAustrian Agency for Health and Food Safety (AGES), Institute for Food Safety Vienna, Department for Molecular Biology and Microbiology, Spargelfeldstrasse 191, 1220 Vienna, AustriaAustrian Agency for Health and Food Safety (AGES), Institute for Food Safety Vienna, Department for Molecular Biology and Microbiology, Spargelfeldstrasse 191, 1220 Vienna, AustriaThe substitution of more appreciated animal species by animal species of lower commercial value is a common type of meat product adulteration. DNA metabarcoding, the combination of DNA barcoding with next-generation sequencing (NGS), plays an increasing role in food authentication. In the present study, we investigated the applicability of a DNA metabarcoding method for routine analysis of mammalian and poultry species in food and pet food products. We analyzed a total of 104 samples (25 reference samples, 56 food products and 23 pet food products) by DNA metabarcoding and by using a commercial DNA array and/or by real-time PCR. The qualitative and quantitative results obtained by the DNA metabarcoding method were in line with those obtained by PCR. Results from the independent analysis of a subset of seven reference samples in two laboratories demonstrate the robustness and reproducibility of the DNA metabarcoding method. DNA metabarcoding is particularly suitable for detecting unexpected species ignored by targeted methods such as real-time PCR and can also be an attractive alternative with respect to the expenses as indicated by current data from the cost accounting of the AGES laboratory. Our results for the commercial samples show that in addition to food products, DNA metabarcoding is particularly applicable to pet food products, which frequently contain multiple animal species and are also highly prone to adulteration as indicated by the high portion of analyzed pet food products containing undeclared species.https://www.mdpi.com/2304-8158/10/11/2875DNA metabarcoding16S rDNAmeat species identificationauthenticationfoodpet food |
| spellingShingle | Laura Preckel Claudia Brünen-Nieweler Grégoire Denay Henning Petersen Margit Cichna-Markl Stefanie Dobrovolny Rupert Hochegger Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding Foods DNA metabarcoding 16S rDNA meat species identification authentication food pet food |
| title | Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding |
| title_full | Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding |
| title_fullStr | Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding |
| title_full_unstemmed | Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding |
| title_short | Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding |
| title_sort | identification of mammalian and poultry species in food and pet food samples using 16s rdna metabarcoding |
| topic | DNA metabarcoding 16S rDNA meat species identification authentication food pet food |
| url | https://www.mdpi.com/2304-8158/10/11/2875 |
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